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- W2000383400 abstract "In this study, we have substituted serine-43 by cysteine in the recombinant citrate synthase from a moderately thermophilic ArchaeonThermoplasma acidophilum,for site-specific attachment of labels and have investigated the effects of this mutation on the biochemical properties and thermal stability of the enzyme. Both wild-type and the mutant enzymes were purified to homogenity using affinity chromatography on Matrex Gel Red A. The mutantThermoplasmacitrate synthase is very similar to wild-type citrate synthase in its substrate and co-factor specificities, pH profile and thermal stability. The mutation, however, has decreased the enzyme activity. The newly introduced reactive sulphydryl group could be easily modified by DTNB and labelled with 4-chloro-7-sulphobenzofuran, without loss of any activity." @default.
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- W2000383400 date "1996-07-01" @default.
- W2000383400 modified "2023-10-18" @default.
- W2000383400 title "The Effect of Cysteine-43 Mutation on Thermostability and Kinetic Properties of Citrate Synthase fromThermoplasma acidophilum" @default.
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- W2000383400 doi "https://doi.org/10.1006/bbrc.1996.1011" @default.
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