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- W2000388449 abstract "The addition of ionic modifiers at low concentration to non-aqueous, primarily methanolic, eluents can facilitate the high-performance liquid chromatographic analysis of a wide range of basic compounds using microparticulate silica columns. The major factors influencing both the selectivity of the system and the retention volumes of individual analytes are the pH and teh ionic strength of the eluent, although changes in the organic component of the eluent can give useful changes in selectivity, as can the use of chemically bonded stationary phases, e.g., octadecylsilyl. In general, retention can be predicted to a large extent by pKa, and even very weak bases such as benzodiazepines can be retained under strongly acidic conditions. These non-aqueous system show high efficiency, stability and reproducibility, and give long column life. In addition, they are suitable for use with low-weavelength UV, fluorescence and electrochemical oxidation detectin. The preparation of the eluent is simple, and the direct analysis of extracts performed using a non-eluting solvent is easily possible. It is clear that these systems have many advantages over bonded-phase systems using aqueous eluents in the liquid chromatographic analysis of basic drugs." @default.
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- W2000388449 date "1982-09-01" @default.
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- W2000388449 title "High performance liquid chromatographic analysis of basic drugs on silica columns using non-aqueous ionic eluents" @default.
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- W2000388449 doi "https://doi.org/10.1016/s0021-9673(00)84853-0" @default.
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