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- W2000393948 abstract "An RNA secondary structure of the bovine leukemia virus (BLV) 5′-terminal RNA sequence was constructed by computer-assisted RNA secondary structure analysis. Mutations were created in the noncoding region (NCR) of BLV, which contains a conserved consensus sequence, to disrupt predicted secondary structure of this region. After transfection of these constructs into FLK-BLV cells and analysis of viral particles a reduction in mutant RNA content was observed relative to that of unmutated vector RNA. The packaging efficiency of the mutant with a substitution in the consensus sequence was reduced threefold and that of the mutant with a deleted 5′ NCR was reduced fivefold. We conclude that predicted RNA secondary structure and/or nucleotide sequence of the BLV noncoding region is essential for BLV RNA packaging in vivo." @default.
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- W2000393948 date "1995-08-01" @default.
- W2000393948 modified "2023-09-27" @default.
- W2000393948 title "An RNA Stem-Loop Structure Involved in the Packaging of Bovine Leukemia Virus Genomic RNA in Vivo" @default.
- W2000393948 doi "https://doi.org/10.1006/viro.1995.1425" @default.
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