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- W2000394712 abstract "The aim of this work was to test the suitability of chemiluminescence (CL) and isothermal microcalorimetry as simple, sensitive, and direct methods for the estimation of oxidation status of phosphatidylcholine in raw material and in lipid-based formulations. Such methods would avoid extensive sample preparation, allowing their use during early development work. Soya phosphatidylcholine (S-PC) was dissolved in medium-chain monoacylglycerol (MCM) and stored at 40°C in air and under nitrogen. Oxidative status was measured at different times by thiobarbituric acid value, iodometry, UV spectroscopy, high performance liquid chromatography (HPLC)-evaporative light scattering (ELS)-UV detection, isothermal microcalorimetry, and direct chemiluminescence. Oxidation parameters were analyzed in terms of the mechanism of oxidation. Data from the various methods were also found to be well correlated with that obtained from direct CL measurements. Induction times of about 21 days were found for a 50/50 w/w S-PC/MCM sample stored in air. MCM was found to have a protective effect on S-PC. Results indicate that both isothermal microcalorimetry and direct CL techniques can be used for estimation of the oxidation levels in phosphatidylcholines and should be applicable to phospholipids in general. MCM is a novel solvent for this purpose. CL measurements have the additional advantages of being rapid and convenient. Specifics of method development have been discussed." @default.
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- W2000394712 date "1996-10-11" @default.
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- W2000394712 title "Chemiluminescence is a rapid and sensitive method to assess phosphatidylcholine oxidation" @default.
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- W2000394712 doi "https://doi.org/10.1016/0378-5173(96)04670-4" @default.
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