FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000394801> ?p ?o ?g. }

• W2000394801 endingPage "123" @default.
• W2000394801 startingPage "115" @default.
• W2000394801 abstract "Pig muscle 3-phosphoglycerate kinase was complexed with 1-anilino-8-naphthalenesulfonate (ANS) in order to monitor the binding of substrates to the enzyme. The enzyme-dye interaction did not influence the enzymic activity under the experimental conditions used. By measuring the substrate-dependent change in the fluorescence emission of ANS molecules tightly bound to the enzyme (Kd less than or equal to 0.05 mM), fluorimetric titrations were carried out in 0.1 M Tris/HCl buffer pH 7.5, containing 5 mM mercaptoethanol, at 20 degrees C. The dissociation constants obtained for the separate bindings of 3-phosphoglycerate, MgATP, 1,3-bisphosphoglycerate and MgADP were 0.03 +/- 0.01 mM, 0.15 +/- 0.10 mM, 0.00005 +/- 0.00001 mM and 0.15 +/- 0.10 mM respectively. binding of 3-phosphoglycerate is weakened when MgATP is also bound to the enzyme: the dissociation constant of 3-phosphoglycerate in this ternary complex (0.25 +/- 0.08 mM) is comparable to its Km value (0.38 +/- 0.10 mM). The same weakening can be observed in the non-productive ternary complexes where MgATP is replaced by MgADP (Kd = 0.20 +/- 0.10 mM) or AMP (Kd = 0.12 +/- 0.05 mM), whereas adenosine has no such effect. This indicates the importance of the negatively charged phosphate(s) of nucleotides in influencing the binding of 3-phosphoglycerate. In contrast to 3-phosphoglycerate, the binding of the substrate analogue, glycerol 3-phosphate is practically not affected by the presence of MgATP: the dissociation constant to the free enzyme (0.40 +/- 0.10 mM) is comparable to its inhibitory constant (0.70 +/- 0.20 mM). This finding and the similarity of the dissociation constant of glycerol 3-phosphate binding (0.40 +/- 0.10 mM) and the Km value of 3-phosphoglycerate (0.38 +/- 0.10 mM) suggest that, during the enzymic reaction, binding of 3-phosphoglycerate occurs probably without involvement of the carboxyl group." @default.
• W2000394801 created "2016-06-24" @default.
• W2000394801 creator A5012234932 @default.
• W2000394801 creator A5020641179 @default.
• W2000394801 date "1984-02-01" @default.
• W2000394801 modified "2023-10-14" @default.
• W2000394801 title "Adenine nucleotides affect the binding of 3-phosphoglycerate to pig muscle 3-phosphoglycerate kinase" @default.
• W2000394801 cites W1510195768 @default.
• W2000394801 cites W1527272231 @default.
• W2000394801 cites W1528597297 @default.
• W2000394801 cites W1538749943 @default.
• W2000394801 cites W1545555367 @default.
• W2000394801 cites W1547655165 @default.
• W2000394801 cites W1548303104 @default.
• W2000394801 cites W1584310946 @default.
• W2000394801 cites W1892754291 @default.
• W2000394801 cites W1893925512 @default.
• W2000394801 cites W1967319998 @default.
• W2000394801 cites W1977832308 @default.
• W2000394801 cites W1980109507 @default.
• W2000394801 cites W1983784919 @default.
• W2000394801 cites W2002511030 @default.
• W2000394801 cites W2002900567 @default.
• W2000394801 cites W2023462249 @default.
• W2000394801 cites W2041659515 @default.
• W2000394801 cites W2043147330 @default.
• W2000394801 cites W2049690848 @default.
• W2000394801 cites W2054061426 @default.
• W2000394801 cites W2064001809 @default.
• W2000394801 cites W2064265793 @default.
• W2000394801 cites W2065201394 @default.
• W2000394801 cites W2068301028 @default.
• W2000394801 cites W2074950656 @default.
• W2000394801 cites W2089147203 @default.
• W2000394801 cites W2094101317 @default.
• W2000394801 cites W2115077137 @default.
• W2000394801 cites W2154094194 @default.
• W2000394801 cites W2154815862 @default.
• W2000394801 cites W2196280832 @default.
• W2000394801 cites W2324693368 @default.
• W2000394801 cites W2413573908 @default.
• W2000394801 cites W8645423 @default.
• W2000394801 cites W943478509 @default.
• W2000394801 doi "https://doi.org/10.1111/j.1432-1033.1984.tb07984.x" @default.
• W2000394801 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6607835" @default.
• W2000394801 hasPublicationYear "1984" @default.
• W2000394801 type Work @default.
• W2000394801 sameAs 2000394801 @default.
• W2000394801 citedByCount "21" @default.
• W2000394801 countsByYear W20003948012021 @default.
• W2000394801 crossrefType "journal-article" @default.
• W2000394801 hasAuthorship W2000394801A5012234932 @default.
• W2000394801 hasAuthorship W2000394801A5020641179 @default.
• W2000394801 hasBestOaLocation W20003948011 @default.
• W2000394801 hasConcept C102931765 @default.
• W2000394801 hasConcept C104317684 @default.
• W2000394801 hasConcept C12554922 @default.
• W2000394801 hasConcept C155911427 @default.
• W2000394801 hasConcept C170493617 @default.
• W2000394801 hasConcept C178790620 @default.
• W2000394801 hasConcept C179104552 @default.
• W2000394801 hasConcept C181199279 @default.
• W2000394801 hasConcept C184866935 @default.
• W2000394801 hasConcept C185592680 @default.
• W2000394801 hasConcept C18903297 @default.
• W2000394801 hasConcept C199360897 @default.
• W2000394801 hasConcept C2776043855 @default.
• W2000394801 hasConcept C2777289219 @default.
• W2000394801 hasConcept C2779564974 @default.
• W2000394801 hasConcept C41008148 @default.
• W2000394801 hasConcept C512185932 @default.
• W2000394801 hasConcept C55493867 @default.
• W2000394801 hasConcept C64452783 @default.
• W2000394801 hasConcept C71240020 @default.
• W2000394801 hasConcept C74998103 @default.
• W2000394801 hasConcept C86756495 @default.
• W2000394801 hasConcept C86803240 @default.
• W2000394801 hasConceptScore W2000394801C102931765 @default.
• W2000394801 hasConceptScore W2000394801C104317684 @default.
• W2000394801 hasConceptScore W2000394801C12554922 @default.
• W2000394801 hasConceptScore W2000394801C155911427 @default.
• W2000394801 hasConceptScore W2000394801C170493617 @default.
• W2000394801 hasConceptScore W2000394801C178790620 @default.
• W2000394801 hasConceptScore W2000394801C179104552 @default.
• W2000394801 hasConceptScore W2000394801C181199279 @default.
• W2000394801 hasConceptScore W2000394801C184866935 @default.
• W2000394801 hasConceptScore W2000394801C185592680 @default.
• W2000394801 hasConceptScore W2000394801C18903297 @default.
• W2000394801 hasConceptScore W2000394801C199360897 @default.
• W2000394801 hasConceptScore W2000394801C2776043855 @default.
• W2000394801 hasConceptScore W2000394801C2777289219 @default.
• W2000394801 hasConceptScore W2000394801C2779564974 @default.
• W2000394801 hasConceptScore W2000394801C41008148 @default.
• W2000394801 hasConceptScore W2000394801C512185932 @default.
• W2000394801 hasConceptScore W2000394801C55493867 @default.
• W2000394801 hasConceptScore W2000394801C64452783 @default.
• W2000394801 hasConceptScore W2000394801C71240020 @default.
• W2000394801 hasConceptScore W2000394801C74998103 @default.

• next