FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000405962> ?p ?o ?g. }

• W2000405962 endingPage "621" @default.
• W2000405962 startingPage "615" @default.
• W2000405962 abstract "Aromatic tuning, i.e. repositioning aromatic residues found at the cytoplasmic end of transmembrane (TM) domains within bacterial receptors, has been previously shown to modulate signal output from the aspartate chemoreceptor (Tar) and the major osmosensor EnvZ of Escherichia coli. In the case of Tar, changes in signal output consistent with the vertical position of the native Trp-Tyr aromatic tandem within TM2 were observed. In contrast, within EnvZ, where a Trp-Leu-Phe aromatic triplet was repositioned, the surface that the triplet resided upon was the major determinant governing signal output. However, these studies failed to determine whether moving the aromatic residues was sufficient to physically reposition the TM helix within a membrane. Recent coarse-grained molecular dynamics (CG-MD) simulations predicted displacement of Tar TM2 upon moving the aromatic residues at the cytoplasmic end of the helix. Here, we demonstrate that repositioning the Trp-Tyr tandem within Tar TM2 displaces the C-terminal boundary of the helix relative to the membrane. In a similar analysis of EnvZ, an abrupt initial displacement of TM2 was observed but no subsequent movement was seen, suggesting that the vertical position of TM2 is not governed by the location of the Trp-Leu-Phe triplet. Our results also provide another set of experimental data, i.e. the resistance of EnvZ TM2 to being displaced upon aromatic tuning, which could be useful for subsequent refinement of the initial CG-MD simulations. Finally, we discuss the limitations of these methodologies, how moving flanking aromatic residues might impact steady-state signal output and the potential to employ aromatic tuning in other bacterial membrane-spanning receptors." @default.
• W2000405962 created "2016-06-24" @default.
• W2000405962 creator A5007682824 @default.
• W2000405962 creator A5014574892 @default.
• W2000405962 creator A5044650505 @default.
• W2000405962 creator A5088287474 @default.
• W2000405962 date "2015-02-01" @default.
• W2000405962 modified "2023-09-27" @default.
• W2000405962 title "Differential repositioning of the second transmembrane helices from E. coli Tar and EnvZ upon moving the flanking aromatic residues" @default.
• W2000405962 cites W1919281320 @default.
• W2000405962 cites W1967125556 @default.
• W2000405962 cites W1969433297 @default.
• W2000405962 cites W1970605146 @default.
• W2000405962 cites W1973204293 @default.
• W2000405962 cites W1980372301 @default.
• W2000405962 cites W1983032969 @default.
• W2000405962 cites W1984640106 @default.
• W2000405962 cites W1990154230 @default.
• W2000405962 cites W1994013119 @default.
• W2000405962 cites W1996478334 @default.
• W2000405962 cites W1999909414 @default.
• W2000405962 cites W2002981234 @default.
• W2000405962 cites W2009301895 @default.
• W2000405962 cites W2010543660 @default.
• W2000405962 cites W2012615687 @default.
• W2000405962 cites W2017139960 @default.
• W2000405962 cites W2024410271 @default.
• W2000405962 cites W2025859600 @default.
• W2000405962 cites W2026078372 @default.
• W2000405962 cites W2031038296 @default.
• W2000405962 cites W2033058355 @default.
• W2000405962 cites W2034418435 @default.
• W2000405962 cites W2040255016 @default.
• W2000405962 cites W2042118984 @default.
• W2000405962 cites W2044189945 @default.
• W2000405962 cites W2044925360 @default.
• W2000405962 cites W2049716406 @default.
• W2000405962 cites W2058426472 @default.
• W2000405962 cites W2059021093 @default.
• W2000405962 cites W2060343318 @default.
• W2000405962 cites W2060772173 @default.
• W2000405962 cites W2065246002 @default.
• W2000405962 cites W2068254342 @default.
• W2000405962 cites W2070383873 @default.
• W2000405962 cites W2071654641 @default.
• W2000405962 cites W2075004481 @default.
• W2000405962 cites W2077396167 @default.
• W2000405962 cites W2077679749 @default.
• W2000405962 cites W2079256912 @default.
• W2000405962 cites W2096254207 @default.
• W2000405962 cites W2100968031 @default.
• W2000405962 cites W2104996488 @default.
• W2000405962 cites W2108949591 @default.
• W2000405962 cites W2115252051 @default.
• W2000405962 cites W2117594322 @default.
• W2000405962 cites W2126654096 @default.
• W2000405962 cites W2152863678 @default.
• W2000405962 cites W2154909249 @default.
• W2000405962 cites W2154941638 @default.
• W2000405962 cites W2161113227 @default.
• W2000405962 cites W2161341952 @default.
• W2000405962 cites W2162378755 @default.
• W2000405962 cites W2329633980 @default.
• W2000405962 doi "https://doi.org/10.1016/j.bbamem.2014.11.017" @default.
• W2000405962 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4281490" @default.
• W2000405962 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25445668" @default.
• W2000405962 hasPublicationYear "2015" @default.
• W2000405962 type Work @default.
• W2000405962 sameAs 2000405962 @default.
• W2000405962 citedByCount "7" @default.
• W2000405962 countsByYear W20004059622015 @default.
• W2000405962 countsByYear W20004059622016 @default.
• W2000405962 countsByYear W20004059622017 @default.
• W2000405962 countsByYear W20004059622018 @default.
• W2000405962 crossrefType "journal-article" @default.
• W2000405962 hasAuthorship W2000405962A5007682824 @default.
• W2000405962 hasAuthorship W2000405962A5014574892 @default.
• W2000405962 hasAuthorship W2000405962A5044650505 @default.
• W2000405962 hasAuthorship W2000405962A5088287474 @default.
• W2000405962 hasBestOaLocation W20004059622 @default.
• W2000405962 hasConcept C118892022 @default.
• W2000405962 hasConcept C12554922 @default.
• W2000405962 hasConcept C170493617 @default.
• W2000405962 hasConcept C185592680 @default.
• W2000405962 hasConcept C18903297 @default.
• W2000405962 hasConcept C192643346 @default.
• W2000405962 hasConcept C199360897 @default.
• W2000405962 hasConcept C24530287 @default.
• W2000405962 hasConcept C2777992278 @default.
• W2000405962 hasConcept C2778530040 @default.
• W2000405962 hasConcept C2779965526 @default.
• W2000405962 hasConcept C41008148 @default.
• W2000405962 hasConcept C515207424 @default.
• W2000405962 hasConcept C55493867 @default.
• W2000405962 hasConcept C86803240 @default.
• W2000405962 hasConceptScore W2000405962C118892022 @default.
• W2000405962 hasConceptScore W2000405962C12554922 @default.
• W2000405962 hasConceptScore W2000405962C170493617 @default.

• next