Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000406704> ?p ?o ?g. }
- W2000406704 endingPage "1419" @default.
- W2000406704 startingPage "1412" @default.
- W2000406704 abstract "Abstract Objectives Infections caused by the rapidly growing mycobacterium (RGM) Mycobacterium abscessus are notoriously difficult to treat due to the innate resistance of M. abscessus to most clinically available antimicrobials. Aminoglycoside antibiotics (AGA) are a cornerstone of antimicrobial chemotherapy against M. abscessus infections, although little is known about intrinsic drug resistance mechanisms. We investigated the role of chromosomally encoded putative aminoglycoside-modifying enzymes (AME) in AGA susceptibility in M. abscessus. Methods Clinical isolates of M. abscessus were tested for susceptibility to a series of AGA with different substituents at positions 2′, 3′ and 4′ of ring 1 in MIC assays. Cell-free extracts of M. abscessus type strain ATCC 19977 and Mycobacterium smegmatis strains SZ380 [aac(2′)-Id+], EP10 [aac(2′)-Id−] and SZ461 [aac(2′)-Id+, rrs A1408G] were investigated for AGA acetylation activity using thin-layer chromatography (TLC). Cell-free ribosome translation assays were performed to directly study drug–target interaction. Results Cell-free translation assays demonstrated that ribosomes of M. abscessus and M. smegmatis show comparable susceptibility to all tested AGA. MIC assays for M. abscessus and M. smegmatis, however, consistently showed the lowest MIC values for 2′-hydroxy-AGA as compared with 2′-amino-AGA, indicating that an aminoglycoside-2′-acetyltransferase, Aac(2′), contributes to innate AGA susceptibility. TLC experiments confirmed enzymatic activity consistent with Aac(2′). Using M. smegmatis as a model for RGM, acetyltransferase activity was shown to be up-regulated in response to AGA-induced inhibition of protein synthesis. Conclusions Our findings point to AME as important determinants of AGA susceptibility in M. abscessus." @default.
- W2000406704 created "2016-06-24" @default.
- W2000406704 creator A5019449593 @default.
- W2000406704 creator A5037949399 @default.
- W2000406704 creator A5048274739 @default.
- W2000406704 creator A5063079076 @default.
- W2000406704 creator A5068194041 @default.
- W2000406704 creator A5071414949 @default.
- W2000406704 creator A5078442567 @default.
- W2000406704 date "2015-01-20" @default.
- W2000406704 modified "2023-09-23" @default.
- W2000406704 title "Aminoglycoside-modifying enzymes determine the innate susceptibility to aminoglycoside antibiotics in rapidly growing mycobacteria" @default.
- W2000406704 cites W1556539514 @default.
- W2000406704 cites W1601962946 @default.
- W2000406704 cites W1960965614 @default.
- W2000406704 cites W1963631541 @default.
- W2000406704 cites W1966121566 @default.
- W2000406704 cites W1967457339 @default.
- W2000406704 cites W1968226573 @default.
- W2000406704 cites W1982921226 @default.
- W2000406704 cites W1983544635 @default.
- W2000406704 cites W2012416775 @default.
- W2000406704 cites W2017989854 @default.
- W2000406704 cites W2026042024 @default.
- W2000406704 cites W2046466506 @default.
- W2000406704 cites W2055126178 @default.
- W2000406704 cites W2070440819 @default.
- W2000406704 cites W2072708417 @default.
- W2000406704 cites W2076822926 @default.
- W2000406704 cites W2078178166 @default.
- W2000406704 cites W2078652451 @default.
- W2000406704 cites W2087442919 @default.
- W2000406704 cites W2093649670 @default.
- W2000406704 cites W2095632243 @default.
- W2000406704 cites W2100254594 @default.
- W2000406704 cites W2104312632 @default.
- W2000406704 cites W2105208344 @default.
- W2000406704 cites W2108435028 @default.
- W2000406704 cites W2108616691 @default.
- W2000406704 cites W2116065463 @default.
- W2000406704 cites W2127531034 @default.
- W2000406704 cites W2129477704 @default.
- W2000406704 cites W2132298801 @default.
- W2000406704 cites W2136307168 @default.
- W2000406704 cites W2141500396 @default.
- W2000406704 cites W2142447693 @default.
- W2000406704 cites W2143231186 @default.
- W2000406704 cites W2145922409 @default.
- W2000406704 cites W2148987519 @default.
- W2000406704 cites W2151636105 @default.
- W2000406704 cites W2154603562 @default.
- W2000406704 cites W2155181416 @default.
- W2000406704 cites W2156886482 @default.
- W2000406704 cites W2157381480 @default.
- W2000406704 cites W2162625328 @default.
- W2000406704 cites W2166283051 @default.
- W2000406704 cites W2171378731 @default.
- W2000406704 cites W2024502922 @default.
- W2000406704 doi "https://doi.org/10.1093/jac/dku550" @default.
- W2000406704 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25604746" @default.
- W2000406704 hasPublicationYear "2015" @default.
- W2000406704 type Work @default.
- W2000406704 sameAs 2000406704 @default.
- W2000406704 citedByCount "19" @default.
- W2000406704 countsByYear W20004067042016 @default.
- W2000406704 countsByYear W20004067042017 @default.
- W2000406704 countsByYear W20004067042018 @default.
- W2000406704 countsByYear W20004067042019 @default.
- W2000406704 countsByYear W20004067042020 @default.
- W2000406704 countsByYear W20004067042021 @default.
- W2000406704 countsByYear W20004067042022 @default.
- W2000406704 countsByYear W20004067042023 @default.
- W2000406704 crossrefType "journal-article" @default.
- W2000406704 hasAuthorship W2000406704A5019449593 @default.
- W2000406704 hasAuthorship W2000406704A5037949399 @default.
- W2000406704 hasAuthorship W2000406704A5048274739 @default.
- W2000406704 hasAuthorship W2000406704A5063079076 @default.
- W2000406704 hasAuthorship W2000406704A5068194041 @default.
- W2000406704 hasAuthorship W2000406704A5071414949 @default.
- W2000406704 hasAuthorship W2000406704A5078442567 @default.
- W2000406704 hasBestOaLocation W20004067042 @default.
- W2000406704 hasConcept C142724271 @default.
- W2000406704 hasConcept C2776111603 @default.
- W2000406704 hasConcept C2777204207 @default.
- W2000406704 hasConcept C2777787772 @default.
- W2000406704 hasConcept C2777975735 @default.
- W2000406704 hasConcept C2780374374 @default.
- W2000406704 hasConcept C2781069245 @default.
- W2000406704 hasConcept C501593827 @default.
- W2000406704 hasConcept C523546767 @default.
- W2000406704 hasConcept C54355233 @default.
- W2000406704 hasConcept C71924100 @default.
- W2000406704 hasConcept C86803240 @default.
- W2000406704 hasConcept C89423630 @default.
- W2000406704 hasConceptScore W2000406704C142724271 @default.
- W2000406704 hasConceptScore W2000406704C2776111603 @default.
- W2000406704 hasConceptScore W2000406704C2777204207 @default.
- W2000406704 hasConceptScore W2000406704C2777787772 @default.