FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000407369> ?p ?o ?g. }

• W2000407369 endingPage "19401" @default.
• W2000407369 startingPage "19396" @default.
• W2000407369 abstract "A method was developed to monitor dynamic changes in protein structure and interfacial behavior on surfaces by single-molecule Förster resonance energy transfer. This method entails the incorporation of unnatural amino acids to site-specifically label proteins with single-molecule Förster resonance energy transfer probes for high-throughput dynamic fluorescence tracking microscopy on surfaces. Structural changes in the enzyme organophosphorus hydrolase (OPH) were monitored upon adsorption to fused silica (FS) surfaces in the presence of BSA on a molecule-by-molecule basis. Analysis of >30,000 individual trajectories enabled the observation of heterogeneities in the kinetics of surface-induced OPH unfolding with unprecedented resolution. In particular, two distinct pathways were observed: a majority population (∼ 85%) unfolded with a characteristic time scale of 0.10 s, and the remainder unfolded more slowly with a time scale of 0.7 s. Importantly, even after unfolding, OPH readily desorbed from FS surfaces, challenging the common notion that surface-induced unfolding leads to irreversible protein binding. This suggests that protein fouling of surfaces is a highly dynamic process because of subtle differences in the adsorption/desorption rates of folded and unfolded species. Moreover, such observations imply that surfaces may act as a source of unfolded (i.e., aggregation-prone) protein back into solution. Continuing study of other proteins and surfaces will examine whether these conclusions are general or specific to OPH in contact with FS. Ultimately, this method, which is widely applicable to virtually any protein, provides the framework to develop surfaces and surface modifications with improved biocompatibility." @default.
• W2000407369 created "2016-06-24" @default.
• W2000407369 creator A5000276486 @default.
• W2000407369 creator A5028132765 @default.
• W2000407369 creator A5044612854 @default.
• W2000407369 creator A5051824598 @default.
• W2000407369 date "2013-11-14" @default.
• W2000407369 modified "2023-10-18" @default.
• W2000407369 title "Single-molecule resolution of protein structure and interfacial dynamics on biomaterial surfaces" @default.
• W2000407369 cites W1969814828 @default.
• W2000407369 cites W1970652428 @default.
• W2000407369 cites W1974618862 @default.
• W2000407369 cites W1983331713 @default.
• W2000407369 cites W1986044859 @default.
• W2000407369 cites W1986826865 @default.
• W2000407369 cites W1990312664 @default.
• W2000407369 cites W1990995901 @default.
• W2000407369 cites W1994014482 @default.
• W2000407369 cites W1994792824 @default.
• W2000407369 cites W2008309511 @default.
• W2000407369 cites W2008601282 @default.
• W2000407369 cites W2012651880 @default.
• W2000407369 cites W2013718333 @default.
• W2000407369 cites W2015442731 @default.
• W2000407369 cites W2021264738 @default.
• W2000407369 cites W2023521131 @default.
• W2000407369 cites W2024328043 @default.
• W2000407369 cites W2025417419 @default.
• W2000407369 cites W2029120593 @default.
• W2000407369 cites W2031094737 @default.
• W2000407369 cites W2031939768 @default.
• W2000407369 cites W2035992571 @default.
• W2000407369 cites W2036133979 @default.
• W2000407369 cites W2040576110 @default.
• W2000407369 cites W2042156094 @default.
• W2000407369 cites W2046324591 @default.
• W2000407369 cites W2047695034 @default.
• W2000407369 cites W2054901066 @default.
• W2000407369 cites W2055758194 @default.
• W2000407369 cites W2060911219 @default.
• W2000407369 cites W2064251172 @default.
• W2000407369 cites W2072330331 @default.
• W2000407369 cites W2081416761 @default.
• W2000407369 cites W2081746001 @default.
• W2000407369 cites W2086724237 @default.
• W2000407369 cites W2100690138 @default.
• W2000407369 cites W2102186623 @default.
• W2000407369 cites W2110114053 @default.
• W2000407369 cites W2125742875 @default.
• W2000407369 cites W2139714252 @default.
• W2000407369 cites W2169290901 @default.
• W2000407369 cites W2280700887 @default.
• W2000407369 cites W2334656813 @default.
• W2000407369 cites W95044234 @default.
• W2000407369 doi "https://doi.org/10.1073/pnas.1311761110" @default.
• W2000407369 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3845135" @default.
• W2000407369 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24235137" @default.
• W2000407369 hasPublicationYear "2013" @default.
• W2000407369 type Work @default.
• W2000407369 sameAs 2000407369 @default.
• W2000407369 citedByCount "38" @default.
• W2000407369 countsByYear W20004073692013 @default.
• W2000407369 countsByYear W20004073692014 @default.
• W2000407369 countsByYear W20004073692015 @default.
• W2000407369 countsByYear W20004073692016 @default.
• W2000407369 countsByYear W20004073692017 @default.
• W2000407369 countsByYear W20004073692018 @default.
• W2000407369 countsByYear W20004073692019 @default.
• W2000407369 countsByYear W20004073692020 @default.
• W2000407369 countsByYear W20004073692021 @default.
• W2000407369 countsByYear W20004073692022 @default.
• W2000407369 crossrefType "journal-article" @default.
• W2000407369 hasAuthorship W2000407369A5000276486 @default.
• W2000407369 hasAuthorship W2000407369A5028132765 @default.
• W2000407369 hasAuthorship W2000407369A5044612854 @default.
• W2000407369 hasAuthorship W2000407369A5051824598 @default.
• W2000407369 hasBestOaLocation W20004073691 @default.
• W2000407369 hasConcept C121332964 @default.
• W2000407369 hasConcept C12554922 @default.
• W2000407369 hasConcept C139447449 @default.
• W2000407369 hasConcept C144024400 @default.
• W2000407369 hasConcept C147597530 @default.
• W2000407369 hasConcept C147789679 @default.
• W2000407369 hasConcept C148898269 @default.
• W2000407369 hasConcept C149923435 @default.
• W2000407369 hasConcept C150394285 @default.
• W2000407369 hasConcept C158617107 @default.
• W2000407369 hasConcept C159467904 @default.
• W2000407369 hasConcept C162711632 @default.
• W2000407369 hasConcept C178790620 @default.
• W2000407369 hasConcept C185592680 @default.
• W2000407369 hasConcept C2908647359 @default.
• W2000407369 hasConcept C32909587 @default.
• W2000407369 hasConcept C47701112 @default.
• W2000407369 hasConcept C55493867 @default.
• W2000407369 hasConcept C59593255 @default.
• W2000407369 hasConcept C62520636 @default.
• W2000407369 hasConcept C8010536 @default.

• next