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- W2000409041 abstract "To analyze the influence of single-stranded template extension of DNA duplex on the conformation of human replication protein A (RPA) bound to DNA we have designed two template-primer systems differing by the size of the single-stranded template tail (9 and 19 nucleotides (nt)). Base-substituted photoreactive dUTP analogs were used as substrates for elongation of radiolabeled template-primer by DNA polymerase beta in the absence or in the presence of RPA. Following UV-crosslinking it was demonstrated that the pattern of RPA subunit labeling and consequently RPA arrangement near the 3'-end of the primer is strongly dependent upon the length of the template extension." @default.
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- W2000409041 date "1998-12-18" @default.
- W2000409041 modified "2023-09-26" @default.
- W2000409041 title "Alternative conformations of human replication protein A are detected by crosslinks with primers carrying a photoreactive group at the 3′-end" @default.
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- W2000409041 doi "https://doi.org/10.1016/s0014-5793(98)01544-0" @default.
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