FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000410904> ?p ?o ?g. }

• W2000410904 endingPage "674" @default.
• W2000410904 startingPage "668" @default.
• W2000410904 abstract "Accurate quantification of deoxyribonucleic acid (DNA) is critical for many analyses in molecular biology and genetic tests. We present a method in which the stoichiometrically existing phosphorus content in purified genomic DNA is quantitatively converted into orthophosphate ions by microwave assisted-UV digestion in the presence of microlitre quantities of dilute reagents (HCl, HNO3, H2O2). The tandem use of microwave energy and ultraviolet photons for DNA digestion in pressurized quartz vessels enables a maximum reaction temperature of 240 °C resulting in efficient and fast mineralization of high molecular weight DNA within 30 minutes. Compared to hotplate digestion, the digestion time is reduced by a factor of 32. The MW-UV sample preparation approach coupled with the ion chromatographic measurement of phosphate using a high performance (HP) methodology provides an accurate quantitation of phosphorus mass fractions as low as 0.3 μg g−1, corresponding to a DNA mass of 25 μg. The relative expanded uncertainties (% U) expressed at 95% confidence for these analyses range from 0.2 to 0.6%. Critically, the matrix of the calibrant solution is also matched with respect to the digested matrix anions (chloride, nitrate), without which significant bias in IC performance is observed. The phosphorus content of the calf thymus DNA was also measured using high-performance inductively coupled plasma optical emission spectroscopy (HP-ICP-OES), which provided independent data for comparison with the MW-UV digestion-IC based approach. Ion chromatography requires smaller volume of materials to perform the analysis and could be useful for characterizing primary calibration standards and certified reference materials with low uncertainties." @default.
• W2000410904 created "2016-06-24" @default.
• W2000410904 creator A5014091677 @default.
• W2000410904 creator A5050408044 @default.
• W2000410904 creator A5058052530 @default.
• W2000410904 date "2012-01-01" @default.
• W2000410904 modified "2023-09-27" @default.
• W2000410904 title "DNA quantification via traceable phosphorus measurement through microwave-assisted UV digestion-ion chromatography" @default.
• W2000410904 cites W1530977725 @default.
• W2000410904 cites W1972406782 @default.
• W2000410904 cites W1972838029 @default.
• W2000410904 cites W1976684284 @default.
• W2000410904 cites W1978295080 @default.
• W2000410904 cites W1983686289 @default.
• W2000410904 cites W1987735739 @default.
• W2000410904 cites W1988604413 @default.
• W2000410904 cites W1995933703 @default.
• W2000410904 cites W1996012148 @default.
• W2000410904 cites W1998882179 @default.
• W2000410904 cites W2001680962 @default.
• W2000410904 cites W2002156882 @default.
• W2000410904 cites W2002390964 @default.
• W2000410904 cites W2004639237 @default.
• W2000410904 cites W2008753573 @default.
• W2000410904 cites W2009554230 @default.
• W2000410904 cites W2021758195 @default.
• W2000410904 cites W2021862109 @default.
• W2000410904 cites W2035904453 @default.
• W2000410904 cites W2043347078 @default.
• W2000410904 cites W2045745464 @default.
• W2000410904 cites W2056392278 @default.
• W2000410904 cites W2063331211 @default.
• W2000410904 cites W2064486535 @default.
• W2000410904 cites W2071363760 @default.
• W2000410904 cites W2157202552 @default.
• W2000410904 cites W2313187827 @default.
• W2000410904 doi "https://doi.org/10.1039/c2an15883c" @default.
• W2000410904 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22159184" @default.
• W2000410904 hasPublicationYear "2012" @default.
• W2000410904 type Work @default.
• W2000410904 sameAs 2000410904 @default.
• W2000410904 citedByCount "11" @default.
• W2000410904 countsByYear W20004109042013 @default.
• W2000410904 countsByYear W20004109042014 @default.
• W2000410904 countsByYear W20004109042015 @default.
• W2000410904 countsByYear W20004109042016 @default.
• W2000410904 countsByYear W20004109042017 @default.
• W2000410904 countsByYear W20004109042021 @default.
• W2000410904 countsByYear W20004109042022 @default.
• W2000410904 crossrefType "journal-article" @default.
• W2000410904 hasAuthorship W2000410904A5014091677 @default.
• W2000410904 hasAuthorship W2000410904A5050408044 @default.
• W2000410904 hasAuthorship W2000410904A5058052530 @default.
• W2000410904 hasConcept C106487976 @default.
• W2000410904 hasConcept C113196181 @default.
• W2000410904 hasConcept C119128265 @default.
• W2000410904 hasConcept C147789679 @default.
• W2000410904 hasConcept C162356407 @default.
• W2000410904 hasConcept C178790620 @default.
• W2000410904 hasConcept C185592680 @default.
• W2000410904 hasConcept C20505706 @default.
• W2000410904 hasConcept C206967267 @default.
• W2000410904 hasConcept C2781357212 @default.
• W2000410904 hasConcept C40875361 @default.
• W2000410904 hasConcept C43617362 @default.
• W2000410904 hasConcept C510538283 @default.
• W2000410904 hasConceptScore W2000410904C106487976 @default.
• W2000410904 hasConceptScore W2000410904C113196181 @default.
• W2000410904 hasConceptScore W2000410904C119128265 @default.
• W2000410904 hasConceptScore W2000410904C147789679 @default.
• W2000410904 hasConceptScore W2000410904C162356407 @default.
• W2000410904 hasConceptScore W2000410904C178790620 @default.
• W2000410904 hasConceptScore W2000410904C185592680 @default.
• W2000410904 hasConceptScore W2000410904C20505706 @default.
• W2000410904 hasConceptScore W2000410904C206967267 @default.
• W2000410904 hasConceptScore W2000410904C2781357212 @default.
• W2000410904 hasConceptScore W2000410904C40875361 @default.
• W2000410904 hasConceptScore W2000410904C43617362 @default.
• W2000410904 hasConceptScore W2000410904C510538283 @default.
• W2000410904 hasIssue "3" @default.
• W2000410904 hasLocation W20004109041 @default.
• W2000410904 hasLocation W20004109042 @default.
• W2000410904 hasOpenAccess W2000410904 @default.
• W2000410904 hasPrimaryLocation W20004109041 @default.
• W2000410904 hasRelatedWork W2007757232 @default.
• W2000410904 hasRelatedWork W2063401993 @default.
• W2000410904 hasRelatedWork W2159789625 @default.
• W2000410904 hasRelatedWork W2357874394 @default.
• W2000410904 hasRelatedWork W2361631570 @default.
• W2000410904 hasRelatedWork W2368673726 @default.
• W2000410904 hasRelatedWork W2376266042 @default.
• W2000410904 hasRelatedWork W2391625483 @default.
• W2000410904 hasRelatedWork W2774147931 @default.
• W2000410904 hasRelatedWork W377763774 @default.
• W2000410904 hasVolume "137" @default.
• W2000410904 isParatext "false" @default.
• W2000410904 isRetracted "false" @default.
• W2000410904 magId "2000410904" @default.

• next