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• W2000413732 abstract "The effects of adrenalectomy (ADX) on the amounts of immunoreactive corticotropin-releasing factor (CRFi) and arginine vasopressin (AVPi) that are stored in the zona externa of the median eminence (ZEME) were investigated by means of quantitative immunocytochemistry. Although ADX of male Wistar rats for 1 week or 4 weeks did not affect CRFi in the ZEME as compared to sham-operated or intact controls, AVPi showed a progressive accumulation. The ratio of AVPi over CRFi in the ZEME had already increased 1 day after ADX. However, it should be noted that the exact changes in CRFi and AVPi as measured by radioimmunoassay and/or quantitative immunocytochemistry were dependent on the substrain of rats used. The secretion rate of CRFi and AVPi was estimated in 1 week and 4 week ADX rats, by measuring the disappearance rate of CRFi and AVPi from the ZEME after blockade of fast axonal transport, by a low non-toxic dose of colchicine (5 micrograms per rat). In contrast to intact rats, where this dose of colchicine did not affect CRFi or AVPi in the ZEME, ADX rats showed a progressive depletion of the CRFi and AVPi stores as measured 2.5 and 5 h later. In 1 week ADX rats, CRFi and AVPi both disappeared at a rate of 7% to 8% of their stores per hour. In contrast, after 4 weeks of ADX the fractional disappearance rates of CRFi and AVPi were different and were 3% and 8% of the content per hour, respectively.(ABSTRACT TRUNCATED AT 250 WORDS)" @default.
• W2000413732 created "2016-06-24" @default.
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• W2000413732 date "1993-02-01" @default.
• W2000413732 modified "2023-09-27" @default.
• W2000413732 title "Is Vasopressin Preferentially Released from Corticotropin-Releasing Factor and Vasopressin Containing Nerve Terminals in the Median Eminence of Adrenalectomized Rats?" @default.
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• W2000413732 doi "https://doi.org/10.1111/j.1365-2826.1993.tb00369.x" @default.
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