FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000427888> ?p ?o ?g. }

• W2000427888 endingPage "533" @default.
• W2000427888 startingPage "521" @default.
• W2000427888 abstract "Although Mycobacterium tuberculosis (M. tb) comprises 11 serine/threonine protein kinases, the mechanisms of regulation of these kinases and the nature of their endogenous substrates remain largely unknown. Herein, we characterized the M. tb kinase PknL by demonstrating that it expresses autophosphorylation activity and phosphorylates Rv2175c. On-target dephosphorylation/MALDI-TOF for identification of phosphorylated peptides was used in combination with LC-ESI/MS/MS for localization of phosphorylation sites. By doing so, five phosphorylated threonine residues were identified in PknL. Among them, we showed that the activation loop phosphorylated residues Thr173 and Thr175 were essential for the autophosphorylation activity of PknL. Phosphorylation of the activation loop Thr173 residue is also required for optimal PknL-mediated phosphorylation of Rv2175c. Together, our results indicate that phosphorylation of the PknL activation loop Thr residues not only controls PknL kinase activity but is also required for recruitment and phosphorylation of its substrate. Rv2175c was found to be phosphorylated when overexpressed and purified from Mycobacterium smegmatis as 2-DE indicated the presence of different phosphorylated isoforms. Given the presence of the dcw gene cluster in the close vicinity of the pknL/Rv2175c locus, and its conservation in all mycobacterial species, we propose that PknL/Rv2175c may represent a functional pair in the regulation of mycobacterial cell division and cell envelope biosynthesis." @default.
• W2000427888 created "2016-06-24" @default.
• W2000427888 creator A5004975943 @default.
• W2000427888 creator A5004980531 @default.
• W2000427888 creator A5036957980 @default.
• W2000427888 creator A5039856849 @default.
• W2000427888 creator A5042762405 @default.
• W2000427888 creator A5056305582 @default.
• W2000427888 creator A5064157453 @default.
• W2000427888 creator A5075671447 @default.
• W2000427888 date "2008-02-01" @default.
• W2000427888 modified "2023-10-15" @default.
• W2000427888 title "TheMycobacterium tuberculosis serine/threonine kinase PknL phosphorylates Rv2175c: Mass spectrometric profiling of the activation loop phosphorylation sites and their role in the recruitment of Rv2175c" @default.
• W2000427888 cites W1529451063 @default.
• W2000427888 cites W1758749763 @default.
• W2000427888 cites W1810897610 @default.
• W2000427888 cites W1970163720 @default.
• W2000427888 cites W1970545310 @default.
• W2000427888 cites W1972893231 @default.
• W2000427888 cites W1975304761 @default.
• W2000427888 cites W1979300827 @default.
• W2000427888 cites W1992055692 @default.
• W2000427888 cites W1992945955 @default.
• W2000427888 cites W1995014241 @default.
• W2000427888 cites W1997601530 @default.
• W2000427888 cites W2006243399 @default.
• W2000427888 cites W2020257412 @default.
• W2000427888 cites W2023516281 @default.
• W2000427888 cites W2039345443 @default.
• W2000427888 cites W2039927551 @default.
• W2000427888 cites W2040594219 @default.
• W2000427888 cites W2043519753 @default.
• W2000427888 cites W2046461084 @default.
• W2000427888 cites W2048392541 @default.
• W2000427888 cites W2049031665 @default.
• W2000427888 cites W2052026185 @default.
• W2000427888 cites W2052448152 @default.
• W2000427888 cites W2055208759 @default.
• W2000427888 cites W2055791119 @default.
• W2000427888 cites W2057069307 @default.
• W2000427888 cites W2058478042 @default.
• W2000427888 cites W2060527882 @default.
• W2000427888 cites W2062173234 @default.
• W2000427888 cites W2062315420 @default.
• W2000427888 cites W2066135800 @default.
• W2000427888 cites W2070034694 @default.
• W2000427888 cites W2071106732 @default.
• W2000427888 cites W2071535444 @default.
• W2000427888 cites W2078290231 @default.
• W2000427888 cites W2078594804 @default.
• W2000427888 cites W2085200152 @default.
• W2000427888 cites W2086987219 @default.
• W2000427888 cites W2091586024 @default.
• W2000427888 cites W2093494886 @default.
• W2000427888 cites W2096221200 @default.
• W2000427888 cites W2102058219 @default.
• W2000427888 cites W2108559568 @default.
• W2000427888 cites W2110695538 @default.
• W2000427888 cites W2113189019 @default.
• W2000427888 cites W2133942178 @default.
• W2000427888 cites W2143724068 @default.
• W2000427888 cites W2145066627 @default.
• W2000427888 cites W2145754107 @default.
• W2000427888 cites W2147566090 @default.
• W2000427888 cites W2158023121 @default.
• W2000427888 cites W2164217177 @default.
• W2000427888 cites W2164517603 @default.
• W2000427888 cites W2165923048 @default.
• W2000427888 cites W2167573033 @default.
• W2000427888 cites W4234722199 @default.
• W2000427888 cites W4295216797 @default.
• W2000427888 doi "https://doi.org/10.1002/pmic.200700442" @default.
• W2000427888 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18175374" @default.
• W2000427888 hasPublicationYear "2008" @default.
• W2000427888 type Work @default.
• W2000427888 sameAs 2000427888 @default.
• W2000427888 citedByCount "57" @default.
• W2000427888 countsByYear W20004278882012 @default.
• W2000427888 countsByYear W20004278882013 @default.
• W2000427888 countsByYear W20004278882014 @default.
• W2000427888 countsByYear W20004278882015 @default.
• W2000427888 countsByYear W20004278882016 @default.
• W2000427888 countsByYear W20004278882017 @default.
• W2000427888 countsByYear W20004278882018 @default.
• W2000427888 countsByYear W20004278882019 @default.
• W2000427888 countsByYear W20004278882020 @default.
• W2000427888 countsByYear W20004278882021 @default.
• W2000427888 countsByYear W20004278882022 @default.
• W2000427888 crossrefType "journal-article" @default.
• W2000427888 hasAuthorship W2000427888A5004975943 @default.
• W2000427888 hasAuthorship W2000427888A5004980531 @default.
• W2000427888 hasAuthorship W2000427888A5036957980 @default.
• W2000427888 hasAuthorship W2000427888A5039856849 @default.
• W2000427888 hasAuthorship W2000427888A5042762405 @default.
• W2000427888 hasAuthorship W2000427888A5056305582 @default.
• W2000427888 hasAuthorship W2000427888A5064157453 @default.
• W2000427888 hasAuthorship W2000427888A5075671447 @default.
• W2000427888 hasConcept C104551158 @default.

• next