Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000431479> ?p ?o ?g. }
Showing items 1 to 93 of
93
with 100 items per page.
- W2000431479 endingPage "43" @default.
- W2000431479 startingPage "35" @default.
- W2000431479 abstract "An enzyme-linked immunosorbent assay (ELISA) has been established using Nunc polystyrene immunoplates coated with a monoclonal antibody to human Tamm-Horsfall urinary glycoprotein (THP) to detect and measure THP in human serum. Optimal reaction conditions for both the monoclonal capture antibody and the affinity-purified rabbit anti-human THP second antibody were established to produce standard curves which showed linearity between 20–90 ng/ml with a sensitivity of 2–3 ng/ml. The plate-to-plate standard curve mean coefficient of variation (CV) was 5.9 ± 2.9% on assays performed on the same day while day to day mean CV was 13.3 ± 2.4%. The specificity of the ELISA was demonstrated by inhibition of binding after preincubation of both urinary THP standards and serum with monoclonal anti-THP antibody. Sera from 195 blood donors tested by the ELISA had a mean concentration of THP antigenic determinants of 260 ± 105 ng/l. Results from three control sera run on all plates used in the survey showed mean CV < 7.6% while no binding was observed with sera from an anephric patient." @default.
- W2000431479 created "2016-06-24" @default.
- W2000431479 creator A5007573464 @default.
- W2000431479 creator A5062137318 @default.
- W2000431479 creator A5067498299 @default.
- W2000431479 creator A5071924075 @default.
- W2000431479 creator A5091014537 @default.
- W2000431479 date "1986-07-01" @default.
- W2000431479 modified "2023-09-27" @default.
- W2000431479 title "A sensitive and specific ELISA using a monoclonal capture antibody for detection of Tamm-Horsfall urinary glycoprotein in serum" @default.
- W2000431479 cites W1232886873 @default.
- W2000431479 cites W1237591984 @default.
- W2000431479 cites W142453141 @default.
- W2000431479 cites W190066489 @default.
- W2000431479 cites W1973435045 @default.
- W2000431479 cites W1986995230 @default.
- W2000431479 cites W2003850645 @default.
- W2000431479 cites W2005813215 @default.
- W2000431479 cites W2029003320 @default.
- W2000431479 cites W2053910286 @default.
- W2000431479 cites W2097339429 @default.
- W2000431479 cites W2100837269 @default.
- W2000431479 cites W2119460480 @default.
- W2000431479 cites W2137185029 @default.
- W2000431479 cites W2168054975 @default.
- W2000431479 cites W2237668592 @default.
- W2000431479 cites W58327633 @default.
- W2000431479 doi "https://doi.org/10.1016/0022-1759(86)90099-2" @default.
- W2000431479 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3722831" @default.
- W2000431479 hasPublicationYear "1986" @default.
- W2000431479 type Work @default.
- W2000431479 sameAs 2000431479 @default.
- W2000431479 citedByCount "12" @default.
- W2000431479 crossrefType "journal-article" @default.
- W2000431479 hasAuthorship W2000431479A5007573464 @default.
- W2000431479 hasAuthorship W2000431479A5062137318 @default.
- W2000431479 hasAuthorship W2000431479A5067498299 @default.
- W2000431479 hasAuthorship W2000431479A5071924075 @default.
- W2000431479 hasAuthorship W2000431479A5091014537 @default.
- W2000431479 hasConcept C108625454 @default.
- W2000431479 hasConcept C153911025 @default.
- W2000431479 hasConcept C159654299 @default.
- W2000431479 hasConcept C163864487 @default.
- W2000431479 hasConcept C185592680 @default.
- W2000431479 hasConcept C203014093 @default.
- W2000431479 hasConcept C2780026642 @default.
- W2000431479 hasConcept C2780420688 @default.
- W2000431479 hasConcept C43617362 @default.
- W2000431479 hasConcept C542903549 @default.
- W2000431479 hasConcept C55493867 @default.
- W2000431479 hasConcept C70784841 @default.
- W2000431479 hasConcept C71924100 @default.
- W2000431479 hasConcept C74460091 @default.
- W2000431479 hasConcept C86803240 @default.
- W2000431479 hasConcept C89838059 @default.
- W2000431479 hasConceptScore W2000431479C108625454 @default.
- W2000431479 hasConceptScore W2000431479C153911025 @default.
- W2000431479 hasConceptScore W2000431479C159654299 @default.
- W2000431479 hasConceptScore W2000431479C163864487 @default.
- W2000431479 hasConceptScore W2000431479C185592680 @default.
- W2000431479 hasConceptScore W2000431479C203014093 @default.
- W2000431479 hasConceptScore W2000431479C2780026642 @default.
- W2000431479 hasConceptScore W2000431479C2780420688 @default.
- W2000431479 hasConceptScore W2000431479C43617362 @default.
- W2000431479 hasConceptScore W2000431479C542903549 @default.
- W2000431479 hasConceptScore W2000431479C55493867 @default.
- W2000431479 hasConceptScore W2000431479C70784841 @default.
- W2000431479 hasConceptScore W2000431479C71924100 @default.
- W2000431479 hasConceptScore W2000431479C74460091 @default.
- W2000431479 hasConceptScore W2000431479C86803240 @default.
- W2000431479 hasConceptScore W2000431479C89838059 @default.
- W2000431479 hasIssue "1" @default.
- W2000431479 hasLocation W20004314791 @default.
- W2000431479 hasLocation W20004314792 @default.
- W2000431479 hasOpenAccess W2000431479 @default.
- W2000431479 hasPrimaryLocation W20004314791 @default.
- W2000431479 hasRelatedWork W1989542447 @default.
- W2000431479 hasRelatedWork W1996802981 @default.
- W2000431479 hasRelatedWork W1998361048 @default.
- W2000431479 hasRelatedWork W1999686187 @default.
- W2000431479 hasRelatedWork W2091749017 @default.
- W2000431479 hasRelatedWork W2329840375 @default.
- W2000431479 hasRelatedWork W2368460920 @default.
- W2000431479 hasRelatedWork W2380360044 @default.
- W2000431479 hasRelatedWork W3201523996 @default.
- W2000431479 hasRelatedWork W4231318786 @default.
- W2000431479 hasVolume "91" @default.
- W2000431479 isParatext "false" @default.
- W2000431479 isRetracted "false" @default.
- W2000431479 magId "2000431479" @default.
- W2000431479 workType "article" @default.