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• W2000431689 abstract "In this work we developed and optimized two molecular-based approaches to monitor rapidly, sensitively and specifically bacterial pathogens from three different genera, Escherichia coli, Pseudomonas aeruginosa, and Salmonella spp., directly in waters. To achieve this aim, firstly a multiplex-PCR assay (M-PCR) was optimized using a primer pair specific for each pathogen. Secondly, as a molecular confirmatory test after isolation of the pathogens by classical microbiological methods, PCR-RFLP of their amplified 16S rDNA genes was performed. It was observed from the results that the developed M-PCR assay has significant impact on the ability to detect sensitively, rapidly and specifically the three pathogens directly in water within a short time (5 h from sampling to obtain final results), therefore it represents a considerable advancement over other known more time-consuming and less-sensitive methods for identification and characterization of these kinds of pathogens." @default.
• W2000431689 created "2016-06-24" @default.
• W2000431689 creator A5003984064 @default.
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• W2000431689 date "2003-01-01" @default.
• W2000431689 modified "2023-09-23" @default.
• W2000431689 title "Multiplex-PCR and PCR-RFLP assays to monitor water quality against pathogenic bacteria" @default.
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• W2000431689 doi "https://doi.org/10.1039/b307476e" @default.
• W2000431689 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/14710924" @default.
• W2000431689 hasPublicationYear "2003" @default.
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