FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000439901> ?p ?o ?g. }

• W2000439901 endingPage "141" @default.
• W2000439901 startingPage "136" @default.
• W2000439901 abstract "Ciliates play important roles as prey and predators in ecosystems. Changes in the ciliate community can affect the composition and population of microfauna and microflora in ecosystems. To investigate the structure of ciliate communities, we developed a nested PCR-DGGE method, which combines a universal eukaryotic-specific primer set in the first PCR step with a ciliate-specific primer set in the second PCR step, to amplify 18S rRNA genes from ciliates. The 300 bp DGGE fragments generated more bands on the gel than the 600 bp DGGE fragments. Prior to bead beating, DNA extraction of ciliates from soil samples was optimized with a combination of freeze-thaw cycles and ultrasonication. We applied this nested PCR-DGGE method to agricultural soils amended with 0, 120, 300, and 600 t ha−1 year−1 of livestock slurry. The results from the DGGE profiles and principal component analysis (PCA) revealed that the supplement of slurry to soils influenced the ciliate communities. From phylogenetic analysis, 108 DGGE bands were assigned to six classes, which included Spirotrichea and Colpodea, of the subphylum Intramacronucleata, and one class of the subphylum Postciliodesmatophora. These results indicated that a wide variety of taxonomic groups were detected by DGGE profiling. Thus, the nested PCR-DGGE method described here could clearly differentiate between ciliate communities within soil samples and allowed for the phylogenetic identification of these ciliates at the class level." @default.
• W2000439901 created "2016-06-24" @default.
• W2000439901 creator A5021798654 @default.
• W2000439901 creator A5021808414 @default.
• W2000439901 creator A5036486827 @default.
• W2000439901 date "2012-01-01" @default.
• W2000439901 modified "2023-10-05" @default.
• W2000439901 title "Application of Nested PCR-DGGE (Denaturing Gradient Gel Electrophoresis) for the Analysis of Ciliate Communities in Soils" @default.
• W2000439901 cites W1598574080 @default.
• W2000439901 cites W1788056442 @default.
• W2000439901 cites W1955694546 @default.
• W2000439901 cites W1968369997 @default.
• W2000439901 cites W1968467165 @default.
• W2000439901 cites W1976287077 @default.
• W2000439901 cites W1990436582 @default.
• W2000439901 cites W1999330489 @default.
• W2000439901 cites W2008921152 @default.
• W2000439901 cites W2014317265 @default.
• W2000439901 cites W2015730017 @default.
• W2000439901 cites W2022659916 @default.
• W2000439901 cites W2030973663 @default.
• W2000439901 cites W2043783333 @default.
• W2000439901 cites W2060571129 @default.
• W2000439901 cites W2066269188 @default.
• W2000439901 cites W2068622882 @default.
• W2000439901 cites W2072438257 @default.
• W2000439901 cites W2078255664 @default.
• W2000439901 cites W2086463820 @default.
• W2000439901 cites W2087792713 @default.
• W2000439901 cites W2089715553 @default.
• W2000439901 cites W2096492229 @default.
• W2000439901 cites W2104051102 @default.
• W2000439901 cites W2115489730 @default.
• W2000439901 cites W2128599049 @default.
• W2000439901 cites W2129575842 @default.
• W2000439901 cites W2132028145 @default.
• W2000439901 cites W2132632499 @default.
• W2000439901 cites W2168484106 @default.
• W2000439901 cites W4232731811 @default.
• W2000439901 cites W4240491816 @default.
• W2000439901 doi "https://doi.org/10.1264/jsme2.me11287" @default.
• W2000439901 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4036011" @default.
• W2000439901 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22791045" @default.
• W2000439901 hasPublicationYear "2012" @default.
• W2000439901 type Work @default.
• W2000439901 sameAs 2000439901 @default.
• W2000439901 citedByCount "14" @default.
• W2000439901 countsByYear W20004399012013 @default.
• W2000439901 countsByYear W20004399012014 @default.
• W2000439901 countsByYear W20004399012015 @default.
• W2000439901 countsByYear W20004399012016 @default.
• W2000439901 countsByYear W20004399012018 @default.
• W2000439901 countsByYear W20004399012020 @default.
• W2000439901 countsByYear W20004399012021 @default.
• W2000439901 countsByYear W20004399012023 @default.
• W2000439901 crossrefType "journal-article" @default.
• W2000439901 hasAuthorship W2000439901A5021798654 @default.
• W2000439901 hasAuthorship W2000439901A5021808414 @default.
• W2000439901 hasAuthorship W2000439901A5036486827 @default.
• W2000439901 hasBestOaLocation W20004399011 @default.
• W2000439901 hasConcept C104317684 @default.
• W2000439901 hasConcept C157431215 @default.
• W2000439901 hasConcept C18903297 @default.
• W2000439901 hasConcept C198549448 @default.
• W2000439901 hasConcept C2777334866 @default.
• W2000439901 hasConcept C42062724 @default.
• W2000439901 hasConcept C49105822 @default.
• W2000439901 hasConcept C54355233 @default.
• W2000439901 hasConcept C59822182 @default.
• W2000439901 hasConcept C86803240 @default.
• W2000439901 hasConceptScore W2000439901C104317684 @default.
• W2000439901 hasConceptScore W2000439901C157431215 @default.
• W2000439901 hasConceptScore W2000439901C18903297 @default.
• W2000439901 hasConceptScore W2000439901C198549448 @default.
• W2000439901 hasConceptScore W2000439901C2777334866 @default.
• W2000439901 hasConceptScore W2000439901C42062724 @default.
• W2000439901 hasConceptScore W2000439901C49105822 @default.
• W2000439901 hasConceptScore W2000439901C54355233 @default.
• W2000439901 hasConceptScore W2000439901C59822182 @default.
• W2000439901 hasConceptScore W2000439901C86803240 @default.
• W2000439901 hasIssue "2" @default.
• W2000439901 hasLocation W20004399011 @default.
• W2000439901 hasLocation W20004399012 @default.
• W2000439901 hasLocation W20004399013 @default.
• W2000439901 hasLocation W20004399014 @default.
• W2000439901 hasOpenAccess W2000439901 @default.
• W2000439901 hasPrimaryLocation W20004399011 @default.
• W2000439901 hasRelatedWork W1973855992 @default.
• W2000439901 hasRelatedWork W1996144811 @default.
• W2000439901 hasRelatedWork W2000439901 @default.
• W2000439901 hasRelatedWork W2000840789 @default.
• W2000439901 hasRelatedWork W2031298991 @default.
• W2000439901 hasRelatedWork W2058004930 @default.
• W2000439901 hasRelatedWork W2077375707 @default.
• W2000439901 hasRelatedWork W2954214984 @default.
• W2000439901 hasRelatedWork W3047216413 @default.
• W2000439901 hasRelatedWork W4306812085 @default.
• W2000439901 hasVolume "27" @default.

• next