Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000442043> ?p ?o ?g. }
Showing items 1 to 97 of
97
with 100 items per page.
- W2000442043 endingPage "413" @default.
- W2000442043 startingPage "401" @default.
- W2000442043 abstract "Catalytically active chymotrypsin derivatives can be synthesized from cyanogen bromide-activated Sephadex G200. In most cases the apparent catalytic activity of the covalently fixed enzyme appears to be considerably decreased in comparison to the activity of the free enzyme. However, by proper choice of the reaction conditions for the activation, enzyme conjugates with high activity, even toward a high molecular substrate, can be synthesized. These latter derivatives may be of practical value for the digestion of proteins. Crosslinked dextran as carrier was chosen because of the possbility, of digesting it enzymatically by dextranase. Sephadex G200, if activated at or below pH 10.3, will combine with chymotrypsin to yield digestable products. Changes of apparent kinetic properties of the fixed enzyme can accordingly be studied during the degradation process. On the solubilization of the insoluble conjugate, a total recovery of activity of the fixed enzyme can be obtained in cases the carrier has been activated by a sufficiently mild procedure. The high apparent Michaelis constant Km of insoluble chymotrypsin–Sephadex toward N-acetyl-L-tyrosine ethyl ester shifts back on solubilization to the value of free chymotrypsin. We therefore propose that the decreased activity of an insoluble chymotrypsin–Sephadex is due to diffusional effects shown by the gel matrix toward the substrate. Similarly observed shifts in optimum pH are explained by accumulation of hydrogen ions in the gel. The organic chemical reaction used for coupling the enzyme to the polymer can therefore be performed without decreasing the inherent catalytic activity of the enzyme. The route described for fixing chymotrypsin to Sephadex followed by solubilization of the products may be useful as a synthetic method for binding proteins, peptides, and other amino group-containing substances to soluble carriers, e.g., for the modification of pharmaceuticals." @default.
- W2000442043 created "2016-06-24" @default.
- W2000442043 creator A5018136220 @default.
- W2000442043 creator A5026019444 @default.
- W2000442043 creator A5040401385 @default.
- W2000442043 date "1970-04-01" @default.
- W2000442043 modified "2023-10-14" @default.
- W2000442043 title "Chemical fixation of chymotrypsin to water-insoluble crosslinked dextran (Sephadex) and solubilization of the enzyme derivatives by means of dextranase" @default.
- W2000442043 cites W131802694 @default.
- W2000442043 cites W1789097051 @default.
- W2000442043 cites W1982625413 @default.
- W2000442043 cites W1987446470 @default.
- W2000442043 cites W1990377796 @default.
- W2000442043 cites W1990533573 @default.
- W2000442043 cites W2009932313 @default.
- W2000442043 cites W2017470279 @default.
- W2000442043 cites W2080779467 @default.
- W2000442043 cites W2088168529 @default.
- W2000442043 cites W2095504882 @default.
- W2000442043 cites W2352812867 @default.
- W2000442043 doi "https://doi.org/10.1002/bip.1970.360090403" @default.
- W2000442043 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/5436167" @default.
- W2000442043 hasPublicationYear "1970" @default.
- W2000442043 type Work @default.
- W2000442043 sameAs 2000442043 @default.
- W2000442043 citedByCount "115" @default.
- W2000442043 countsByYear W20004420432014 @default.
- W2000442043 countsByYear W20004420432020 @default.
- W2000442043 countsByYear W20004420432022 @default.
- W2000442043 crossrefType "journal-article" @default.
- W2000442043 hasAuthorship W2000442043A5018136220 @default.
- W2000442043 hasAuthorship W2000442043A5026019444 @default.
- W2000442043 hasAuthorship W2000442043A5040401385 @default.
- W2000442043 hasConcept C104317684 @default.
- W2000442043 hasConcept C111368507 @default.
- W2000442043 hasConcept C127313418 @default.
- W2000442043 hasConcept C134306372 @default.
- W2000442043 hasConcept C161790260 @default.
- W2000442043 hasConcept C163994747 @default.
- W2000442043 hasConcept C167625842 @default.
- W2000442043 hasConcept C178790620 @default.
- W2000442043 hasConcept C181199279 @default.
- W2000442043 hasConcept C185592680 @default.
- W2000442043 hasConcept C197336794 @default.
- W2000442043 hasConcept C2776183398 @default.
- W2000442043 hasConcept C2777093645 @default.
- W2000442043 hasConcept C2777289219 @default.
- W2000442043 hasConcept C2777339698 @default.
- W2000442043 hasConcept C2778401398 @default.
- W2000442043 hasConcept C2780340462 @default.
- W2000442043 hasConcept C33633552 @default.
- W2000442043 hasConcept C33923547 @default.
- W2000442043 hasConcept C43617362 @default.
- W2000442043 hasConcept C55493867 @default.
- W2000442043 hasConceptScore W2000442043C104317684 @default.
- W2000442043 hasConceptScore W2000442043C111368507 @default.
- W2000442043 hasConceptScore W2000442043C127313418 @default.
- W2000442043 hasConceptScore W2000442043C134306372 @default.
- W2000442043 hasConceptScore W2000442043C161790260 @default.
- W2000442043 hasConceptScore W2000442043C163994747 @default.
- W2000442043 hasConceptScore W2000442043C167625842 @default.
- W2000442043 hasConceptScore W2000442043C178790620 @default.
- W2000442043 hasConceptScore W2000442043C181199279 @default.
- W2000442043 hasConceptScore W2000442043C185592680 @default.
- W2000442043 hasConceptScore W2000442043C197336794 @default.
- W2000442043 hasConceptScore W2000442043C2776183398 @default.
- W2000442043 hasConceptScore W2000442043C2777093645 @default.
- W2000442043 hasConceptScore W2000442043C2777289219 @default.
- W2000442043 hasConceptScore W2000442043C2777339698 @default.
- W2000442043 hasConceptScore W2000442043C2778401398 @default.
- W2000442043 hasConceptScore W2000442043C2780340462 @default.
- W2000442043 hasConceptScore W2000442043C33633552 @default.
- W2000442043 hasConceptScore W2000442043C33923547 @default.
- W2000442043 hasConceptScore W2000442043C43617362 @default.
- W2000442043 hasConceptScore W2000442043C55493867 @default.
- W2000442043 hasIssue "4" @default.
- W2000442043 hasLocation W20004420431 @default.
- W2000442043 hasLocation W20004420432 @default.
- W2000442043 hasOpenAccess W2000442043 @default.
- W2000442043 hasPrimaryLocation W20004420431 @default.
- W2000442043 hasRelatedWork W2024047110 @default.
- W2000442043 hasRelatedWork W2121999736 @default.
- W2000442043 hasRelatedWork W2122982655 @default.
- W2000442043 hasRelatedWork W2170946482 @default.
- W2000442043 hasRelatedWork W2369914366 @default.
- W2000442043 hasRelatedWork W2391873197 @default.
- W2000442043 hasRelatedWork W2400724767 @default.
- W2000442043 hasRelatedWork W2461543754 @default.
- W2000442043 hasRelatedWork W3194615894 @default.
- W2000442043 hasRelatedWork W4282923522 @default.
- W2000442043 hasVolume "9" @default.
- W2000442043 isParatext "false" @default.
- W2000442043 isRetracted "false" @default.
- W2000442043 magId "2000442043" @default.
- W2000442043 workType "article" @default.