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- W2000453423 abstract "Abstract Cytoplasmic fragments, prepared by microdissection of cells from a human amnion cell line, are motile and undergo pinocytosis. They incorporate amino acids but do not incorporate those ribonucleic acid precursors so far tested. Cultures were infected with ornithosis virus before or after preparation of nonucleate cytoplasmic pieces and (1) stained (Giemsa or acridine orange), or (2) labeled with tritiated nucleosides, digested with ribonuclease, stained by the Feulgen method and covered with autoradiographic stripping film. Attachment, penetration, initiation of virus growth and moderately advanced maturation of virus inclusions occurred whether the prospective cytoplasmic fragment was part of an intact cell at early points in this sequence or was already amputated before infection. Synthesis of viral deoxyribonucleic acid was demonstrated by autoradiography. Nuclear participation in the infectious process was not required at any time during the events studied here, but may be necessary for renewal of cytoplasmic materials which are equally essential to functional survival of cytoplasm alone." @default.
- W2000453423 created "2016-06-24" @default.
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- W2000453423 date "1963-01-01" @default.
- W2000453423 modified "2023-09-27" @default.
- W2000453423 title "Subcellular cultivation of a virus: Growth of ornithosis virus in nonnucleate cytoplasm" @default.
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- W2000453423 doi "https://doi.org/10.1016/0042-6822(63)90020-5" @default.
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