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- W2000456872 abstract "Living donor kidneys from spouses and children (from offspring to parents) are currently considered to be important organ sources. However, pregnancy-induced alloimmunization may provoke acute rejection episodes after kidney transplantation. being flow cytometry cross-match (FCXM) we studied donor-specific antibodies (DSAs) in the sera of recipients planned for living kidney transplantation from their spouse or children. When the FCXM was positive, we confirmed the existence of anti-human leukocyte antigen (HLA) antibodies using flow cytometry panel-reactive antibody (flow-PRA). Between March 2005 and November 2010, we tested 85 pretransplantation sera from renal transplant recipients for DSAs. The recipients included 37 wives (group I) and 48 husbands (group II). FCXM-positive sera were tested using a flow-PRA screening method using HLA class I and class II antigen-coated beads. The mean recipient age was 48.1 ± 9.8 (range, 28–69) years and the mean donor age was 45.1 ± 11.1 (range, 23–69 years). Among group I were 18 (48.6%) FCXM-positive cross-matches; for group II, 5 (10.4%) cases (P = .001). Sensitized patients were 37.9% FCXM-positive, whereas nonsensitized patients were 3.7% positive (P = .001). FCXM-positive patients were re-evaluated for anti-HLA antibodies using flow-PRA. Seventeen of 18 group I tests (94.4%) were FCXM-positive, whereas 3 of 5 (60%) were positive among group II. We concluded that flow cytometry–based cross-match and PRA techniques can be used to detect anti-HLA antibodies using spousal or children donors for kidney transplantation." @default.
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- W2000456872 date "2012-07-01" @default.
- W2000456872 modified "2023-09-25" @default.
- W2000456872 title "Flow Cytometric Evaluation of Pregnancy-Induced Anti-Donor Immunization" @default.
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- W2000456872 doi "https://doi.org/10.1016/j.transproceed.2012.04.027" @default.
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