Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000457007> ?p ?o ?g. }
Showing items 1 to 89 of
89
with 100 items per page.
- W2000457007 endingPage "402" @default.
- W2000457007 startingPage "397" @default.
- W2000457007 abstract "The enzyme S-adenosylmethionine:protein carboxyl-O-methyl-transferase, type II (EC2.1.1.77; PCMT) from eukaryotes methyl esterifies peptides containing isoAsp residues, which can arise from spontaneous deamidation of labile Asn residues. We report here a study on in vitro methyl esterification of mouse EGF by bovine brain PCMT. This peptide contains two Asn in the sequences Asn1-Ser2 and Asn16-Gly17. It is known from the literature that the presence of a small residue on the carboxyl side of asparaginyl makes this residue susceptible to deamidation through the spontaneous formation of a succinimide intermediate. Therefore EGF was incubated under deamidating conditions (pH9.0, 37° for 48 h) and the extent of deamidation monitored by enzymatically measuring the NH3 produced during the alkali treatment: a release of 0.80 mol NH3/mol EGF was calculated. The alkali-treated EGF, analyzed by anion-exchange chromatography, shows two major components identified as native EGF (nEGF) and its deamidated form (dEGF). When incubated in the presence of purified PCMT neither nEGF nor dEGF showed any methyl accepting capability. Since it is known that the three-dimensional structure of a protein may hinder the methyl esterification of a potential ethyl accepting site, dEGF was unfolded by reducing and alkylating the intrachain disulfide bridges. Only a slight increase in the methyl accepting capability could be observed. Conversely, when EGF was deamidated after its unfolding, the resulting protein was stoichiometrically methylated by PCMT, presumably at level of isoAsp16. Our findings strongly suggest that the three-dimensional structure of a protein is a major specificity determinant for both deamidation and methyl esterification processes." @default.
- W2000457007 created "2016-06-24" @default.
- W2000457007 creator A5009842064 @default.
- W2000457007 creator A5023707747 @default.
- W2000457007 creator A5024312903 @default.
- W2000457007 creator A5029197815 @default.
- W2000457007 creator A5041594587 @default.
- W2000457007 date "2009-01-12" @default.
- W2000457007 modified "2023-10-17" @default.
- W2000457007 title "Enzymatic methyl esterification of a deamidated form of mouse epidermal growth factor" @default.
- W2000457007 cites W1030517862 @default.
- W2000457007 cites W1498071142 @default.
- W2000457007 cites W1521198514 @default.
- W2000457007 cites W1549296443 @default.
- W2000457007 cites W1559866584 @default.
- W2000457007 cites W1577986704 @default.
- W2000457007 cites W1579529625 @default.
- W2000457007 cites W1583648119 @default.
- W2000457007 cites W1944789189 @default.
- W2000457007 cites W1968145792 @default.
- W2000457007 cites W1968888330 @default.
- W2000457007 cites W1985375832 @default.
- W2000457007 cites W1989965884 @default.
- W2000457007 cites W2016497865 @default.
- W2000457007 cites W2022818851 @default.
- W2000457007 cites W2031035482 @default.
- W2000457007 cites W2034298059 @default.
- W2000457007 cites W2040539292 @default.
- W2000457007 cites W2062270874 @default.
- W2000457007 cites W2069887441 @default.
- W2000457007 cites W2081158966 @default.
- W2000457007 cites W2176699929 @default.
- W2000457007 cites W2616263013 @default.
- W2000457007 cites W3027146158 @default.
- W2000457007 cites W31465123 @default.
- W2000457007 cites W87003019 @default.
- W2000457007 cites W183484090 @default.
- W2000457007 doi "https://doi.org/10.1111/j.1399-3011.1989.tb00215.x" @default.
- W2000457007 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2789201" @default.
- W2000457007 hasPublicationYear "2009" @default.
- W2000457007 type Work @default.
- W2000457007 sameAs 2000457007 @default.
- W2000457007 citedByCount "17" @default.
- W2000457007 crossrefType "journal-article" @default.
- W2000457007 hasAuthorship W2000457007A5009842064 @default.
- W2000457007 hasAuthorship W2000457007A5023707747 @default.
- W2000457007 hasAuthorship W2000457007A5024312903 @default.
- W2000457007 hasAuthorship W2000457007A5029197815 @default.
- W2000457007 hasAuthorship W2000457007A5041594587 @default.
- W2000457007 hasConcept C170493617 @default.
- W2000457007 hasConcept C181199279 @default.
- W2000457007 hasConcept C185592680 @default.
- W2000457007 hasConcept C2776362946 @default.
- W2000457007 hasConcept C2779281246 @default.
- W2000457007 hasConcept C2780784506 @default.
- W2000457007 hasConcept C2781338088 @default.
- W2000457007 hasConcept C55493867 @default.
- W2000457007 hasConcept C71240020 @default.
- W2000457007 hasConceptScore W2000457007C170493617 @default.
- W2000457007 hasConceptScore W2000457007C181199279 @default.
- W2000457007 hasConceptScore W2000457007C185592680 @default.
- W2000457007 hasConceptScore W2000457007C2776362946 @default.
- W2000457007 hasConceptScore W2000457007C2779281246 @default.
- W2000457007 hasConceptScore W2000457007C2780784506 @default.
- W2000457007 hasConceptScore W2000457007C2781338088 @default.
- W2000457007 hasConceptScore W2000457007C55493867 @default.
- W2000457007 hasConceptScore W2000457007C71240020 @default.
- W2000457007 hasIssue "6" @default.
- W2000457007 hasLocation W20004570071 @default.
- W2000457007 hasLocation W20004570072 @default.
- W2000457007 hasOpenAccess W2000457007 @default.
- W2000457007 hasPrimaryLocation W20004570071 @default.
- W2000457007 hasRelatedWork W2030113604 @default.
- W2000457007 hasRelatedWork W2036345118 @default.
- W2000457007 hasRelatedWork W2045449545 @default.
- W2000457007 hasRelatedWork W2075019601 @default.
- W2000457007 hasRelatedWork W2085520623 @default.
- W2000457007 hasRelatedWork W2085698632 @default.
- W2000457007 hasRelatedWork W2086819077 @default.
- W2000457007 hasRelatedWork W2107792670 @default.
- W2000457007 hasRelatedWork W2947721917 @default.
- W2000457007 hasRelatedWork W4300786619 @default.
- W2000457007 hasVolume "33" @default.
- W2000457007 isParatext "false" @default.
- W2000457007 isRetracted "false" @default.
- W2000457007 magId "2000457007" @default.
- W2000457007 workType "article" @default.