FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000463900> ?p ?o ?g. }

• W2000463900 endingPage "1451" @default.
• W2000463900 startingPage "1442" @default.
• W2000463900 abstract "The immobilization of cells within alginate-poly-l-lysine-alginate (APA) microcapsules has been demonstrated to be an effective technology design for long term delivery of therapeutic products. Despite promising advances, biosafety aspects still remain to be improved. Here, we describe a complete characterization of the strategy based on TGL triple-fusion reporter gene--which codifies for Herpes Simplex virus type 1 thymidine-kinase (HSV1-TK), green fluorescent protein (GFP) and Firefly Luciferase--(SFG(NES)TGL) to inactivate encapsulated cells and their therapeutic effects. Myoblasts genetically engineered to secrete erythropoietin (EPO) were retroviraly transduced with the SFG(NES)TGL plasmid to further characterize their ganciclovir (GCV)-mediated inactivation process. GCV sensitivity of encapsulated cells was 100-fold lower when compared to cells plated onto 2D surfaces. However, the number of cells per capsule and EPO secretion decayed to less than 15% at the same time that proliferation was arrested after 14 days of GCV treatment in vitro. In vivo, ten days of GCV treatment was enough to restore the increased hematocrit levels of mice implanted with encapsulated TGL-expressing and EPO-secreting cells. Altogether, these results show that TGL triple-fusion reporter gene may be a good starting point in the search of a suitable biosafety strategy to inactivate encapsulated cells and control their therapeutic effects." @default.
• W2000463900 created "2016-06-24" @default.
• W2000463900 creator A5019952256 @default.
• W2000463900 creator A5031536814 @default.
• W2000463900 creator A5037574771 @default.
• W2000463900 creator A5051292821 @default.
• W2000463900 creator A5070577695 @default.
• W2000463900 creator A5089899406 @default.
• W2000463900 date "2013-01-01" @default.
• W2000463900 modified "2023-10-16" @default.
• W2000463900 title "Inactivation of encapsulated cells and their therapeutic effects by means of TGL triple-fusion reporter/biosafety gene" @default.
• W2000463900 cites W1515378450 @default.
• W2000463900 cites W1963753820 @default.
• W2000463900 cites W1973232010 @default.
• W2000463900 cites W1976340920 @default.
• W2000463900 cites W1991723371 @default.
• W2000463900 cites W1995976170 @default.
• W2000463900 cites W1998899566 @default.
• W2000463900 cites W1999784325 @default.
• W2000463900 cites W2000280419 @default.
• W2000463900 cites W2002522963 @default.
• W2000463900 cites W2012468528 @default.
• W2000463900 cites W2013559933 @default.
• W2000463900 cites W2016873394 @default.
• W2000463900 cites W2018054628 @default.
• W2000463900 cites W2020766415 @default.
• W2000463900 cites W2021856189 @default.
• W2000463900 cites W2022282748 @default.
• W2000463900 cites W2044441655 @default.
• W2000463900 cites W2048476369 @default.
• W2000463900 cites W2053353941 @default.
• W2000463900 cites W2054412308 @default.
• W2000463900 cites W2057691895 @default.
• W2000463900 cites W2060686520 @default.
• W2000463900 cites W2062955516 @default.
• W2000463900 cites W2066370524 @default.
• W2000463900 cites W2068069968 @default.
• W2000463900 cites W2068655992 @default.
• W2000463900 cites W2069880178 @default.
• W2000463900 cites W2072169469 @default.
• W2000463900 cites W2080464780 @default.
• W2000463900 cites W2091330369 @default.
• W2000463900 cites W2096993232 @default.
• W2000463900 cites W2110617603 @default.
• W2000463900 cites W2119103578 @default.
• W2000463900 cites W2126559501 @default.
• W2000463900 cites W2135364083 @default.
• W2000463900 cites W2146789542 @default.
• W2000463900 cites W2164587556 @default.
• W2000463900 cites W2167815042 @default.
• W2000463900 cites W40753975 @default.
• W2000463900 doi "https://doi.org/10.1016/j.biomaterials.2012.10.076" @default.
• W2000463900 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23174140" @default.
• W2000463900 hasPublicationYear "2013" @default.
• W2000463900 type Work @default.
• W2000463900 sameAs 2000463900 @default.
• W2000463900 citedByCount "14" @default.
• W2000463900 countsByYear W20004639002013 @default.
• W2000463900 countsByYear W20004639002014 @default.
• W2000463900 countsByYear W20004639002015 @default.
• W2000463900 countsByYear W20004639002016 @default.
• W2000463900 countsByYear W20004639002017 @default.
• W2000463900 countsByYear W20004639002018 @default.
• W2000463900 countsByYear W20004639002019 @default.
• W2000463900 countsByYear W20004639002020 @default.
• W2000463900 countsByYear W20004639002021 @default.
• W2000463900 crossrefType "journal-article" @default.
• W2000463900 hasAuthorship W2000463900A5019952256 @default.
• W2000463900 hasAuthorship W2000463900A5031536814 @default.
• W2000463900 hasAuthorship W2000463900A5037574771 @default.
• W2000463900 hasAuthorship W2000463900A5051292821 @default.
• W2000463900 hasAuthorship W2000463900A5070577695 @default.
• W2000463900 hasAuthorship W2000463900A5089899406 @default.
• W2000463900 hasConcept C104317684 @default.
• W2000463900 hasConcept C111335760 @default.
• W2000463900 hasConcept C111599444 @default.
• W2000463900 hasConcept C135983454 @default.
• W2000463900 hasConcept C142613039 @default.
• W2000463900 hasConcept C150194340 @default.
• W2000463900 hasConcept C153911025 @default.
• W2000463900 hasConcept C161733203 @default.
• W2000463900 hasConcept C185592680 @default.
• W2000463900 hasConcept C203014093 @default.
• W2000463900 hasConcept C2522874641 @default.
• W2000463900 hasConcept C2777965375 @default.
• W2000463900 hasConcept C2779820661 @default.
• W2000463900 hasConcept C54009773 @default.
• W2000463900 hasConcept C54355233 @default.
• W2000463900 hasConcept C55493867 @default.
• W2000463900 hasConcept C81885089 @default.
• W2000463900 hasConcept C86803240 @default.
• W2000463900 hasConceptScore W2000463900C104317684 @default.
• W2000463900 hasConceptScore W2000463900C111335760 @default.
• W2000463900 hasConceptScore W2000463900C111599444 @default.
• W2000463900 hasConceptScore W2000463900C135983454 @default.
• W2000463900 hasConceptScore W2000463900C142613039 @default.
• W2000463900 hasConceptScore W2000463900C150194340 @default.
• W2000463900 hasConceptScore W2000463900C153911025 @default.

• next