FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000464995> ?p ?o ?g. }

• W2000464995 endingPage "13338" @default.
• W2000464995 startingPage "13328" @default.
• W2000464995 abstract "The RNA-binding protein L7Ae, known for its role in translation (as part of ribosomes) and RNA modification (as part of sn/oRNPs), has also been identified as a subunit of archaeal RNase P, a ribonucleoprotein complex that employs an RNA catalyst for the Mg(2+)-dependent 5' maturation of tRNAs. To better understand the assembly and catalysis of archaeal RNase P, we used a site-specific hydroxyl radical-mediated footprinting strategy to pinpoint the binding sites of Pyrococcus furiosus (Pfu) L7Ae on its cognate RNase P RNA (RPR). L7Ae derivatives with single-Cys substitutions at residues in the predicted RNA-binding interface (K42C/C71V, R46C/C71V, V95C/C71V) were modified with an iron complex of EDTA-2-aminoethyl 2-pyridyl disulfide. Upon addition of hydrogen peroxide and ascorbate, these L7Ae-tethered nucleases were expected to cleave the RPR at nucleotides proximal to the EDTA-Fe-modified residues. Indeed, footprinting experiments with an enzyme assembled with the Pfu RPR and five protein cofactors (POP5, RPP21, RPP29, RPP30 and L7Ae-EDTA-Fe) revealed specific RNA cleavages, localizing the binding sites of L7Ae to the RPR's catalytic and specificity domains. These results support the presence of two kink-turns, the structural motifs recognized by L7Ae, in distinct functional domains of the RPR and suggest testable mechanisms by which L7Ae contributes to RNase P catalysis." @default.
• W2000464995 created "2016-06-24" @default.
• W2000464995 creator A5003714594 @default.
• W2000464995 creator A5018961230 @default.
• W2000464995 creator A5082288662 @default.
• W2000464995 creator A5090020474 @default.
• W2000464995 date "2014-10-31" @default.
• W2000464995 modified "2023-09-28" @default.
• W2000464995 title "The L7Ae protein binds to two kink-turns in the Pyrococcus furiosus RNase P RNA" @default.
• W2000464995 cites W144745942 @default.
• W2000464995 cites W1530278321 @default.
• W2000464995 cites W1961427846 @default.
• W2000464995 cites W1964290593 @default.
• W2000464995 cites W1964594770 @default.
• W2000464995 cites W1966145105 @default.
• W2000464995 cites W1977608471 @default.
• W2000464995 cites W1981707682 @default.
• W2000464995 cites W1983145423 @default.
• W2000464995 cites W1986221874 @default.
• W2000464995 cites W1986639299 @default.
• W2000464995 cites W1990630809 @default.
• W2000464995 cites W1990912560 @default.
• W2000464995 cites W1997769408 @default.
• W2000464995 cites W1998262675 @default.
• W2000464995 cites W1998740180 @default.
• W2000464995 cites W2003467309 @default.
• W2000464995 cites W2005707439 @default.
• W2000464995 cites W2006870002 @default.
• W2000464995 cites W2009040736 @default.
• W2000464995 cites W2009193892 @default.
• W2000464995 cites W2011589271 @default.
• W2000464995 cites W2013039688 @default.
• W2000464995 cites W2017932915 @default.
• W2000464995 cites W2021666355 @default.
• W2000464995 cites W2023957480 @default.
• W2000464995 cites W2024580573 @default.
• W2000464995 cites W2027354207 @default.
• W2000464995 cites W2034761258 @default.
• W2000464995 cites W2036480824 @default.
• W2000464995 cites W2051794062 @default.
• W2000464995 cites W2059594507 @default.
• W2000464995 cites W2065155358 @default.
• W2000464995 cites W2065183541 @default.
• W2000464995 cites W2066527832 @default.
• W2000464995 cites W2067447313 @default.
• W2000464995 cites W2067780078 @default.
• W2000464995 cites W2071641969 @default.
• W2000464995 cites W2072319136 @default.
• W2000464995 cites W2081415752 @default.
• W2000464995 cites W2081784841 @default.
• W2000464995 cites W2083902847 @default.
• W2000464995 cites W2088546096 @default.
• W2000464995 cites W2091399197 @default.
• W2000464995 cites W2099986542 @default.
• W2000464995 cites W2104343747 @default.
• W2000464995 cites W2105113868 @default.
• W2000464995 cites W2117971449 @default.
• W2000464995 cites W2126634558 @default.
• W2000464995 cites W2130076705 @default.
• W2000464995 cites W2130817246 @default.
• W2000464995 cites W2132129663 @default.
• W2000464995 cites W2146841180 @default.
• W2000464995 cites W2152243797 @default.
• W2000464995 cites W2159843086 @default.
• W2000464995 cites W2160420644 @default.
• W2000464995 cites W2169853432 @default.
• W2000464995 cites W2170314520 @default.
• W2000464995 cites W4240292448 @default.
• W2000464995 doi "https://doi.org/10.1093/nar/gku994" @default.
• W2000464995 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4245976" @default.
• W2000464995 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25361963" @default.
• W2000464995 hasPublicationYear "2014" @default.
• W2000464995 type Work @default.
• W2000464995 sameAs 2000464995 @default.
• W2000464995 citedByCount "14" @default.
• W2000464995 countsByYear W20004649952016 @default.
• W2000464995 countsByYear W20004649952017 @default.
• W2000464995 countsByYear W20004649952018 @default.
• W2000464995 countsByYear W20004649952019 @default.
• W2000464995 countsByYear W20004649952020 @default.
• W2000464995 countsByYear W20004649952021 @default.
• W2000464995 countsByYear W20004649952022 @default.
• W2000464995 crossrefType "journal-article" @default.
• W2000464995 hasAuthorship W2000464995A5003714594 @default.
• W2000464995 hasAuthorship W2000464995A5018961230 @default.
• W2000464995 hasAuthorship W2000464995A5082288662 @default.
• W2000464995 hasAuthorship W2000464995A5090020474 @default.
• W2000464995 hasBestOaLocation W20004649951 @default.
• W2000464995 hasConcept C104317684 @default.
• W2000464995 hasConcept C107824862 @default.
• W2000464995 hasConcept C10879258 @default.
• W2000464995 hasConcept C2776943354 @default.
• W2000464995 hasConcept C2778021871 @default.
• W2000464995 hasConcept C3017666073 @default.
• W2000464995 hasConcept C41282012 @default.
• W2000464995 hasConcept C550995028 @default.
• W2000464995 hasConcept C552990157 @default.
• W2000464995 hasConcept C55493867 @default.

• next