Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000470225> ?p ?o ?g. }
Showing items 1 to 61 of
61
with 100 items per page.
- W2000470225 endingPage "209" @default.
- W2000470225 startingPage "209" @default.
- W2000470225 abstract "The arsonous acids melarsen oxide and pyridinyl-3-arsonous acid were successfully applied for chemical trapping of otherwise tran sient (un)folding intermediates. The trapped and arsonous acid-modified peptides and proteins were amenable to direct molecular weight determination by mass spectrometry, e.g. by nanoelectrospray ionization mass spectrometry or matrix-assisted laser desorption/ionization mass spectrometry. Arsonous acids exhibited several advantageous features such as (i) cross-linking two closely spaced thiol groups providing detailed tertiary structure information, (ii) high solubility as monomeric and oligomeric deriva tives in aqueous solution, (iii) adding a relatively large mass increment to proteins upon single modification and (iv) performing fast and specific modification of bis-thiol groups in proteins to form stable structures without any side reactions even with a high molar excess of arsonous acids. Bis-cysteine thiol-selective modification of vasopressin with pyridinyl-3-arsonous acid was confirmed by Fourier transform ion cyclotron resonance mass spectrometry measurements. The major product obtained by denaturing unfolding of bovine pancreatic trypsin inhibitor (BPTI) and trapping with melarsen oxide eluted like folded BPTI in high-performance liquid chromatography analyses, indicating a “native-like” structure of this folding intermediate. The results obtained from the investigated model compounds suggest a general applicability of arsonous acids for selective bis-thiol modifications, enabling the study of protein folding reactions by mass spectrometry." @default.
- W2000470225 created "2016-06-24" @default.
- W2000470225 creator A5007056143 @default.
- W2000470225 creator A5046015932 @default.
- W2000470225 date "1998-01-01" @default.
- W2000470225 modified "2023-09-26" @default.
- W2000470225 title "A mass spectrometric approach to the characterization of protein folding reactions" @default.
- W2000470225 cites W1967767087 @default.
- W2000470225 doi "https://doi.org/10.1255/ejms.212" @default.
- W2000470225 hasPublicationYear "1998" @default.
- W2000470225 type Work @default.
- W2000470225 sameAs 2000470225 @default.
- W2000470225 citedByCount "6" @default.
- W2000470225 crossrefType "journal-article" @default.
- W2000470225 hasAuthorship W2000470225A5007056143 @default.
- W2000470225 hasAuthorship W2000470225A5046015932 @default.
- W2000470225 hasConcept C119599485 @default.
- W2000470225 hasConcept C127413603 @default.
- W2000470225 hasConcept C147597530 @default.
- W2000470225 hasConcept C162356407 @default.
- W2000470225 hasConcept C171250308 @default.
- W2000470225 hasConcept C185592680 @default.
- W2000470225 hasConcept C192562407 @default.
- W2000470225 hasConcept C204328495 @default.
- W2000470225 hasConcept C2776545253 @default.
- W2000470225 hasConcept C2780841128 @default.
- W2000470225 hasConcept C43617362 @default.
- W2000470225 hasConcept C55493867 @default.
- W2000470225 hasConceptScore W2000470225C119599485 @default.
- W2000470225 hasConceptScore W2000470225C127413603 @default.
- W2000470225 hasConceptScore W2000470225C147597530 @default.
- W2000470225 hasConceptScore W2000470225C162356407 @default.
- W2000470225 hasConceptScore W2000470225C171250308 @default.
- W2000470225 hasConceptScore W2000470225C185592680 @default.
- W2000470225 hasConceptScore W2000470225C192562407 @default.
- W2000470225 hasConceptScore W2000470225C204328495 @default.
- W2000470225 hasConceptScore W2000470225C2776545253 @default.
- W2000470225 hasConceptScore W2000470225C2780841128 @default.
- W2000470225 hasConceptScore W2000470225C43617362 @default.
- W2000470225 hasConceptScore W2000470225C55493867 @default.
- W2000470225 hasIssue "1" @default.
- W2000470225 hasLocation W20004702251 @default.
- W2000470225 hasOpenAccess W2000470225 @default.
- W2000470225 hasPrimaryLocation W20004702251 @default.
- W2000470225 hasRelatedWork W2044817072 @default.
- W2000470225 hasRelatedWork W2114589162 @default.
- W2000470225 hasRelatedWork W2157006014 @default.
- W2000470225 hasRelatedWork W2160995247 @default.
- W2000470225 hasRelatedWork W2611898430 @default.
- W2000470225 hasRelatedWork W2626988399 @default.
- W2000470225 hasRelatedWork W2791118179 @default.
- W2000470225 hasRelatedWork W2795803259 @default.
- W2000470225 hasRelatedWork W3037456415 @default.
- W2000470225 hasRelatedWork W4250819211 @default.
- W2000470225 hasVolume "4" @default.
- W2000470225 isParatext "false" @default.
- W2000470225 isRetracted "false" @default.
- W2000470225 magId "2000470225" @default.
- W2000470225 workType "article" @default.