FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000473225> ?p ?o ?g. }

• W2000473225 endingPage "3387" @default.
• W2000473225 startingPage "3375" @default.
• W2000473225 abstract "A transgenic Cassie (CA) line of Yorkshire (YK) pigs was developed using a transgene composed of the mouse parotid secretory protein promoter linked to the Escherichia coli phytase gene integrated in chromosome 4. Previous studies documented that salivary secretion of phytase was sufficient to enable efficient digestion of plant feed phytate P. In the present study the catalytic properties and tissue distribution of the phytase in CA pigs were determined by a combination of enzymatic assays, immunohistochemistry, and immunoblots of tissue samples. The E. coli phytase had a mass of 44.82 kDa whereas the phytase secreted in CA saliva had a mass of 52.42 kDa as a result of glycosylation of the enzyme in the parotid gland. Despite the difference in size, the 2 enzymes exhibited similar substrate specificities, and substrate affinity (Km) and maximum hydrolytic activity (Vmax) catalytic properties. Phytase assays showed that the enzyme was present at high specific activity in the salivary glands with low activity in the soft palate and essentially none in the kidney, lean (muscle), liver, or skin of CA pigs and none in YK pigs. This conclusion was supported by immunoblot analysis using a polyclonal anti-phytase antibody. Immunohistochemical analysis of 83 different tissue locations of CA and YK pigs confirmed the ubiquitous presence of phytase in serous cells of the salivary glands and the localized presence of phytase in both serous and mixed cell types in the submucosal glands of the oropharynx; in the pharynx, tonsils, and esophagus; in some Bowman's glands in the nasal mucosa and eustachian tube; and in the prostate gland of CA boars. Furthermore, it showed the absence of phytase from the kidney, lean, liver, and skin of CA pigs. Phytase was not detected in any of the conventional YK tissues tested. The phytase was found to be glycosylated with the allergenic galactose-α-1,3-galactose (α-gal) epitope by immunoblotting using α-gal specific monoclonal antibodies. Galactose-α-1,3-galactose glycosylation of proteins is a common feature of pork and other red meats. The α-gal epitope was shown to be associated with a few proteins in muscle and skin but with the greatest number of proteins in kidney and parotid tissues of CA and YK pigs. The absence of phytase from the major food tissues and the displacement of other α-gal glycosylated proteins in the parotid glands by α-gal glycosylated phytase in conjunction with previously published data support the contention that expression of the novel phytase has minimal influence on pork quality and safety." @default.
• W2000473225 created "2016-06-24" @default.
• W2000473225 creator A5003780805 @default.
• W2000473225 creator A5008066585 @default.
• W2000473225 creator A5009704442 @default.
• W2000473225 creator A5022360146 @default.
• W2000473225 creator A5035918736 @default.
• W2000473225 creator A5040848887 @default.
• W2000473225 creator A5049175823 @default.
• W2000473225 creator A5054085661 @default.
• W2000473225 creator A5068575502 @default.
• W2000473225 creator A5070345399 @default.
• W2000473225 date "2014-08-01" @default.
• W2000473225 modified "2023-09-26" @default.
• W2000473225 title "Phytase properties and locations in tissues of transgenic pigs secreting phytase in the saliva1" @default.
• W2000473225 cites W1493444364 @default.
• W2000473225 cites W1508475184 @default.
• W2000473225 cites W1965570469 @default.
• W2000473225 cites W1966912604 @default.
• W2000473225 cites W1971922540 @default.
• W2000473225 cites W1984367265 @default.
• W2000473225 cites W1994309360 @default.
• W2000473225 cites W2013512676 @default.
• W2000473225 cites W2016404707 @default.
• W2000473225 cites W2029122422 @default.
• W2000473225 cites W2033008851 @default.
• W2000473225 cites W2038764767 @default.
• W2000473225 cites W2040820272 @default.
• W2000473225 cites W2053327247 @default.
• W2000473225 cites W2053580931 @default.
• W2000473225 cites W2056728361 @default.
• W2000473225 cites W2060929865 @default.
• W2000473225 cites W2080175095 @default.
• W2000473225 cites W2094964805 @default.
• W2000473225 cites W2100837269 @default.
• W2000473225 cites W2127857928 @default.
• W2000473225 cites W2132910660 @default.
• W2000473225 cites W2145769148 @default.
• W2000473225 cites W2149725254 @default.
• W2000473225 cites W2155458663 @default.
• W2000473225 cites W2231614804 @default.
• W2000473225 cites W2271002965 @default.
• W2000473225 cites W2334650013 @default.
• W2000473225 cites W4232534952 @default.
• W2000473225 cites W4241490718 @default.
• W2000473225 doi "https://doi.org/10.2527/jas.2014-7782" @default.
• W2000473225 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24902597" @default.
• W2000473225 hasPublicationYear "2014" @default.
• W2000473225 type Work @default.
• W2000473225 sameAs 2000473225 @default.
• W2000473225 citedByCount "9" @default.
• W2000473225 countsByYear W20004732252018 @default.
• W2000473225 countsByYear W20004732252019 @default.
• W2000473225 countsByYear W20004732252020 @default.
• W2000473225 countsByYear W20004732252021 @default.
• W2000473225 countsByYear W20004732252022 @default.
• W2000473225 countsByYear W20004732252023 @default.
• W2000473225 crossrefType "journal-article" @default.
• W2000473225 hasAuthorship W2000473225A5003780805 @default.
• W2000473225 hasAuthorship W2000473225A5008066585 @default.
• W2000473225 hasAuthorship W2000473225A5009704442 @default.
• W2000473225 hasAuthorship W2000473225A5022360146 @default.
• W2000473225 hasAuthorship W2000473225A5035918736 @default.
• W2000473225 hasAuthorship W2000473225A5040848887 @default.
• W2000473225 hasAuthorship W2000473225A5049175823 @default.
• W2000473225 hasAuthorship W2000473225A5054085661 @default.
• W2000473225 hasAuthorship W2000473225A5068575502 @default.
• W2000473225 hasAuthorship W2000473225A5070345399 @default.
• W2000473225 hasConcept C109551439 @default.
• W2000473225 hasConcept C153911025 @default.
• W2000473225 hasConcept C159654299 @default.
• W2000473225 hasConcept C181199279 @default.
• W2000473225 hasConcept C203014093 @default.
• W2000473225 hasConcept C204232928 @default.
• W2000473225 hasConcept C2778937882 @default.
• W2000473225 hasConcept C504460877 @default.
• W2000473225 hasConcept C55493867 @default.
• W2000473225 hasConcept C86803240 @default.
• W2000473225 hasConcept C956191 @default.
• W2000473225 hasConceptScore W2000473225C109551439 @default.
• W2000473225 hasConceptScore W2000473225C153911025 @default.
• W2000473225 hasConceptScore W2000473225C159654299 @default.
• W2000473225 hasConceptScore W2000473225C181199279 @default.
• W2000473225 hasConceptScore W2000473225C203014093 @default.
• W2000473225 hasConceptScore W2000473225C204232928 @default.
• W2000473225 hasConceptScore W2000473225C2778937882 @default.
• W2000473225 hasConceptScore W2000473225C504460877 @default.
• W2000473225 hasConceptScore W2000473225C55493867 @default.
• W2000473225 hasConceptScore W2000473225C86803240 @default.
• W2000473225 hasConceptScore W2000473225C956191 @default.
• W2000473225 hasIssue "8" @default.
• W2000473225 hasLocation W20004732251 @default.
• W2000473225 hasLocation W20004732252 @default.
• W2000473225 hasOpenAccess W2000473225 @default.
• W2000473225 hasPrimaryLocation W20004732251 @default.
• W2000473225 hasRelatedWork W1984799404 @default.
• W2000473225 hasRelatedWork W2228698594 @default.
• W2000473225 hasRelatedWork W2362958935 @default.

• next