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- W2000473469 abstract "The effect of soluble, polymerizing and polymerized des-AA and des-AABB fibrin upon platelets of citrated, platelet-rich plasma(ci-PRP) has been studied in the aggregometer. Preformed fibrin monomers, made from human fibrinogen in urea incubated with insolubilized reptilase or thrombin, were added to ci-PRP in soluble amounts or in amounts resulting in the formation of visible fibrin, respectively. To study the effect of polymerized fibrin, fibrin monomers were first allowed to clot upon dilution with buffer, and subsequent to harvesting and homogenization of the clots, added to ci-PRP. Only des-AA and des-AABB fibrin monomers polymerizing to form visible fibrin, aggregated platelets, whilst soluble and polymerized fibrin had no such effect. The platelet aggregation induced by polymerizing fibrin was completely inhibited by apyrase, indicating that release of platelet ADP was necessary for fibrin-induced platelet aggregation. Beta-thromboglobulin-RIA determinations in ci-PRP and EDTA-PRP exposed to polymerizing fibrin further substantiated platelet release reaction, whilst corresponding determinations in fibrin-challenged KCN- or acetylsalicylic acid-treated ci-PRP did not disclose any sign of platelet release. In contrast to platelets of ci-PRP, platelets of EDTA-PRP, KCN- or acetylsalicylic acid-treated ci-PRP were not aggregated by polymerizing fibrin." @default.
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- W2000473469 date "1980-04-01" @default.
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- W2000473469 title "Interaction of platelets with soluble, polymerizing and polymerized des-AA and des-AABB fibrin in human plasma" @default.
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- W2000473469 doi "https://doi.org/10.1016/0049-3848(80)90176-0" @default.
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