FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000474140> ?p ?o ?g. }

• W2000474140 endingPage "389" @default.
• W2000474140 startingPage "382" @default.
• W2000474140 abstract "A procedure was developed to estimate protein concentrations using color image analysis of protein spots stained with ponceau S. The method involved spotting a constant volume (2 μl) of the protein solutions on nitrocellulose paper, staining with acidic ponceau S, destaining, and air drying the paper. The image of the nitrocellulose paper was grabbed using a digital color scanner and thresholded with an optimal value to mark the area of the spot. The intensity of the color in the spot was measured in an arbitrary unit of intensity termed as inverse integrated gray value. This value showed a discernible increase with protein concentrations from 0.1 to 50 μg protein per spot (0.05–25 mg/ml). The method is simple and convenient compared to the conventional spectrophotometric procedures and allows several samples to be analyzed simultaneously. It can also be used to estimate protein concentration in the spots stained with Coomassie brilliant blue or other dyes." @default.
• W2000474140 created "2016-06-24" @default.
• W2000474140 creator A5007123952 @default.
• W2000474140 creator A5013831910 @default.
• W2000474140 creator A5058997129 @default.
• W2000474140 creator A5064937652 @default.
• W2000474140 creator A5081196995 @default.
• W2000474140 date "1999-02-01" @default.
• W2000474140 modified "2023-09-27" @default.
• W2000474140 title "Protein Determination by Ponceau S Using Digital Color Image Analysis of Protein Spots on Nitrocellulose Membranes" @default.
• W2000474140 cites W1775749144 @default.
• W2000474140 cites W1963613583 @default.
• W2000474140 cites W1990583552 @default.
• W2000474140 cites W2004831921 @default.
• W2000474140 cites W2009596830 @default.
• W2000474140 cites W2010107472 @default.
• W2000474140 cites W2011345042 @default.
• W2000474140 cites W2022510326 @default.
• W2000474140 cites W2022720578 @default.
• W2000474140 cites W2026121316 @default.
• W2000474140 cites W2029411830 @default.
• W2000474140 cites W2038209064 @default.
• W2000474140 cites W2072160174 @default.
• W2000474140 cites W2087376507 @default.
• W2000474140 cites W2088447195 @default.
• W2000474140 cites W2412970387 @default.
• W2000474140 cites W4293247451 @default.
• W2000474140 doi "https://doi.org/10.1006/abio.1998.3020" @default.
• W2000474140 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10036145" @default.
• W2000474140 hasPublicationYear "1999" @default.
• W2000474140 type Work @default.
• W2000474140 sameAs 2000474140 @default.
• W2000474140 citedByCount "56" @default.
• W2000474140 countsByYear W20004741402012 @default.
• W2000474140 countsByYear W20004741402013 @default.
• W2000474140 countsByYear W20004741402014 @default.
• W2000474140 countsByYear W20004741402015 @default.
• W2000474140 countsByYear W20004741402016 @default.
• W2000474140 countsByYear W20004741402018 @default.
• W2000474140 countsByYear W20004741402019 @default.
• W2000474140 countsByYear W20004741402020 @default.
• W2000474140 countsByYear W20004741402021 @default.
• W2000474140 countsByYear W20004741402022 @default.
• W2000474140 countsByYear W20004741402023 @default.
• W2000474140 crossrefType "journal-article" @default.
• W2000474140 hasAuthorship W2000474140A5007123952 @default.
• W2000474140 hasAuthorship W2000474140A5013831910 @default.
• W2000474140 hasAuthorship W2000474140A5058997129 @default.
• W2000474140 hasAuthorship W2000474140A5064937652 @default.
• W2000474140 hasAuthorship W2000474140A5081196995 @default.
• W2000474140 hasConcept C113196181 @default.
• W2000474140 hasConcept C120665830 @default.
• W2000474140 hasConcept C121332964 @default.
• W2000474140 hasConcept C147789679 @default.
• W2000474140 hasConcept C185592680 @default.
• W2000474140 hasConcept C2778954975 @default.
• W2000474140 hasConcept C2779506182 @default.
• W2000474140 hasConcept C2780761556 @default.
• W2000474140 hasConcept C2781255879 @default.
• W2000474140 hasConcept C41625074 @default.
• W2000474140 hasConcept C43617362 @default.
• W2000474140 hasConcept C54355233 @default.
• W2000474140 hasConcept C55493867 @default.
• W2000474140 hasConcept C74864618 @default.
• W2000474140 hasConcept C86803240 @default.
• W2000474140 hasConceptScore W2000474140C113196181 @default.
• W2000474140 hasConceptScore W2000474140C120665830 @default.
• W2000474140 hasConceptScore W2000474140C121332964 @default.
• W2000474140 hasConceptScore W2000474140C147789679 @default.
• W2000474140 hasConceptScore W2000474140C185592680 @default.
• W2000474140 hasConceptScore W2000474140C2778954975 @default.
• W2000474140 hasConceptScore W2000474140C2779506182 @default.
• W2000474140 hasConceptScore W2000474140C2780761556 @default.
• W2000474140 hasConceptScore W2000474140C2781255879 @default.
• W2000474140 hasConceptScore W2000474140C41625074 @default.
• W2000474140 hasConceptScore W2000474140C43617362 @default.
• W2000474140 hasConceptScore W2000474140C54355233 @default.
• W2000474140 hasConceptScore W2000474140C55493867 @default.
• W2000474140 hasConceptScore W2000474140C74864618 @default.
• W2000474140 hasConceptScore W2000474140C86803240 @default.
• W2000474140 hasIssue "2" @default.
• W2000474140 hasLocation W20004741401 @default.
• W2000474140 hasLocation W20004741402 @default.
• W2000474140 hasOpenAccess W2000474140 @default.
• W2000474140 hasPrimaryLocation W20004741401 @default.
• W2000474140 hasRelatedWork W1963557714 @default.
• W2000474140 hasRelatedWork W1971005624 @default.
• W2000474140 hasRelatedWork W2000474140 @default.
• W2000474140 hasRelatedWork W2023496436 @default.
• W2000474140 hasRelatedWork W2045121112 @default.
• W2000474140 hasRelatedWork W2089361409 @default.
• W2000474140 hasRelatedWork W2121704691 @default.
• W2000474140 hasRelatedWork W2365046854 @default.
• W2000474140 hasRelatedWork W2977675980 @default.
• W2000474140 hasRelatedWork W3010622974 @default.
• W2000474140 hasVolume "267" @default.
• W2000474140 isParatext "false" @default.
• W2000474140 isRetracted "false" @default.

• next