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• W2000484135 abstract "The expression of aromatase mRNA in cultured mouse brain cells was measured by a quantitative reverse transcription-PCR method using an internal standard. Aromatase mRNA was expressed in the cultured neurons prepared from diencephalon at 0.037 ± 0.005attomol/μg total RNA. However, the mRNA was detected in neither the neurons from cerebral cortex nor astrocytes. These results demonstrate that expression of aromatase mRNA is regulated in cell type- and region-specific manners in cultured brain cells. The aromatase mRNA levels in neurons obtained from diencephalon were not affected by administration of testosterone, estradiol, dexamethasone, forskolin, or 12-O-tetradecanoyl 13-acetate. The results are in apparent disagreement with previous reports concerning regulation by androgens of brain aromatase activity in vivo and may suggest that aromatase expression in brain neurons is not directly induced by androgens. Androgen induction of brain aromatase may be mediated by several steps including cell-cell (neuron-neuron and/or neuron-glia) interaction." @default.
• W2000484135 created "2016-06-24" @default.
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• W2000484135 date "1994-07-01" @default.
• W2000484135 modified "2023-09-27" @default.
• W2000484135 title "Cell type- and region-specific expression of aromatase mRNA in cultured brain cells" @default.
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• W2000484135 doi "https://doi.org/10.1016/0169-328x(94)90127-9" @default.
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