FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000485935> ?p ?o ?g. }

• W2000485935 endingPage "486" @default.
• W2000485935 startingPage "480" @default.
• W2000485935 abstract "The alkane-1-monoxygenase (alkB) complex of Pseudomonas putida GPo1 has been extensively studied in the past and shown to be capable of oxidising aliphatic C5–C12 alkanes to primary alcohols both in the wild-type organism by growth on C5–C12 alkanes as sole carbon source and in vitro. Despite this, successful n-dodecane oxidation for the production of 1-dodecanol or dodecanoic acid has proven elusive in the past when using alkB-expressing recombinants. This article demonstrates, for the first time in vivo, by using the Escherichia coli GEC137 pGEc47ΔJ strain, that n-dodecane oxidation using this enzyme for the production of primary alcohols and carboxylic acids is feasible and in fact potentially more promising than n-octane oxidation due to lower product and substrate toxicity. Yields are reported of 1-dodecanol of up to 2 g/Lorganic and dodecanoic acid up to 19.7 g/Lorganic in a 2 L stirred tank reactor with 1 L aqueous phase and 200 mL of n-dodecane as a second phase. The maximum volumetric rate of combined alcohol and acid production achieved was 1.9 g/Lorganic/h (0.35 g/Ltotal/h). The maximum specific activity of combined alcohol and acid production was 7-fold lower on n-dodecane (3.5 μmol/min/gdcw) than on n-octane (21 μmol/min/gdcw); similar to the 5-fold difference observed between wild-type growth rates using the two respective alkanes as sole carbon source. Despite this, both total volumetric rate and final yield exceeded n-octane oxidation by 3.5-fold under the same conditions, due to the lower toxicity of n-dodecane and its oxidation products to E. coli compared to the 8-carbon equivalents. Substrate access limitations and the overoxidation of 1-dodecanol to dodecanoic acid were identified as the most important limitations to be addressed." @default.
• W2000485935 created "2016-06-24" @default.
• W2000485935 creator A5036903025 @default.
• W2000485935 creator A5070645740 @default.
• W2000485935 creator A5080117913 @default.
• W2000485935 date "2011-05-01" @default.
• W2000485935 modified "2023-10-14" @default.
• W2000485935 title "Whole-cell bio-oxidation of n-dodecane using the alkane hydroxylase system of P. putida GPo1 expressed in E. coli" @default.
• W2000485935 cites W1493556845 @default.
• W2000485935 cites W1603429441 @default.
• W2000485935 cites W1606979654 @default.
• W2000485935 cites W1623529556 @default.
• W2000485935 cites W1709457880 @default.
• W2000485935 cites W1974105595 @default.
• W2000485935 cites W2005826917 @default.
• W2000485935 cites W2050107614 @default.
• W2000485935 cites W2055242720 @default.
• W2000485935 cites W2061083574 @default.
• W2000485935 cites W2071588717 @default.
• W2000485935 cites W2078654991 @default.
• W2000485935 cites W2080665106 @default.
• W2000485935 cites W2083518261 @default.
• W2000485935 cites W2085738044 @default.
• W2000485935 cites W2086780301 @default.
• W2000485935 cites W2093261949 @default.
• W2000485935 cites W2100837269 @default.
• W2000485935 cites W2134183258 @default.
• W2000485935 cites W2146184051 @default.
• W2000485935 cites W2146307401 @default.
• W2000485935 cites W2150471285 @default.
• W2000485935 cites W2154884438 @default.
• W2000485935 cites W2163727020 @default.
• W2000485935 cites W2164658997 @default.
• W2000485935 cites W2171075490 @default.
• W2000485935 cites W4213261135 @default.
• W2000485935 cites W4293247451 @default.
• W2000485935 cites W4317640870 @default.
• W2000485935 doi "https://doi.org/10.1016/j.enzmictec.2011.01.008" @default.
• W2000485935 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22113020" @default.
• W2000485935 hasPublicationYear "2011" @default.
• W2000485935 type Work @default.
• W2000485935 sameAs 2000485935 @default.
• W2000485935 citedByCount "38" @default.
• W2000485935 countsByYear W20004859352012 @default.
• W2000485935 countsByYear W20004859352013 @default.
• W2000485935 countsByYear W20004859352014 @default.
• W2000485935 countsByYear W20004859352015 @default.
• W2000485935 countsByYear W20004859352016 @default.
• W2000485935 countsByYear W20004859352017 @default.
• W2000485935 countsByYear W20004859352018 @default.
• W2000485935 countsByYear W20004859352019 @default.
• W2000485935 countsByYear W20004859352020 @default.
• W2000485935 countsByYear W20004859352022 @default.
• W2000485935 countsByYear W20004859352023 @default.
• W2000485935 crossrefType "journal-article" @default.
• W2000485935 hasAuthorship W2000485935A5036903025 @default.
• W2000485935 hasAuthorship W2000485935A5070645740 @default.
• W2000485935 hasAuthorship W2000485935A5080117913 @default.
• W2000485935 hasConcept C104317684 @default.
• W2000485935 hasConcept C134121241 @default.
• W2000485935 hasConcept C13965031 @default.
• W2000485935 hasConcept C178790620 @default.
• W2000485935 hasConcept C181199279 @default.
• W2000485935 hasConcept C185592680 @default.
• W2000485935 hasConcept C191897082 @default.
• W2000485935 hasConcept C192562407 @default.
• W2000485935 hasConcept C2775846362 @default.
• W2000485935 hasConcept C2776624427 @default.
• W2000485935 hasConcept C2777207669 @default.
• W2000485935 hasConcept C2777721518 @default.
• W2000485935 hasConcept C2780543182 @default.
• W2000485935 hasConcept C2781066024 @default.
• W2000485935 hasConcept C2781327020 @default.
• W2000485935 hasConcept C547475151 @default.
• W2000485935 hasConcept C55493867 @default.
• W2000485935 hasConcept C71240020 @default.
• W2000485935 hasConceptScore W2000485935C104317684 @default.
• W2000485935 hasConceptScore W2000485935C134121241 @default.
• W2000485935 hasConceptScore W2000485935C13965031 @default.
• W2000485935 hasConceptScore W2000485935C178790620 @default.
• W2000485935 hasConceptScore W2000485935C181199279 @default.
• W2000485935 hasConceptScore W2000485935C185592680 @default.
• W2000485935 hasConceptScore W2000485935C191897082 @default.
• W2000485935 hasConceptScore W2000485935C192562407 @default.
• W2000485935 hasConceptScore W2000485935C2775846362 @default.
• W2000485935 hasConceptScore W2000485935C2776624427 @default.
• W2000485935 hasConceptScore W2000485935C2777207669 @default.
• W2000485935 hasConceptScore W2000485935C2777721518 @default.
• W2000485935 hasConceptScore W2000485935C2780543182 @default.
• W2000485935 hasConceptScore W2000485935C2781066024 @default.
• W2000485935 hasConceptScore W2000485935C2781327020 @default.
• W2000485935 hasConceptScore W2000485935C547475151 @default.
• W2000485935 hasConceptScore W2000485935C55493867 @default.
• W2000485935 hasConceptScore W2000485935C71240020 @default.
• W2000485935 hasIssue "6-7" @default.
• W2000485935 hasLocation W20004859351 @default.
• W2000485935 hasLocation W20004859352 @default.
• W2000485935 hasOpenAccess W2000485935 @default.

• next