FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000495523> ?p ?o ?g. }

• W2000495523 endingPage "77" @default.
• W2000495523 startingPage "65" @default.
• W2000495523 abstract "Because of its high sensitivity, bioluminescence (BL) is an excellent alternative to radioactive quantitation of cytokine RT-PCR-derived products. BL also allows detection of amplicons at cycle numbers not normally detectable using radioactivity. No direct comparisons between these two methods have been made. In this study, the sensitivities of BL using recombinant aequorin, a flash-type luminescent tag capable of detecting signal to attomolar (10−18 M) levels and radio imaging (RI) were directly compared. In addition, the application of BL for detecting cytokine message from biologic samples was examined. BL was 30- to 60-fold more sensitive than RI in detecting human IL-2 and CD3δ amplicons. This difference was particularly found during low cycle PCR, but was less at higher cycle numbers. The ability of BL to detect differences in cytokine message in stimulated and unstimulated human peripheral blood mononuclear cells was also evaluated. Using linear regression analysis, we observed up to 5,000-fold increases in RT-PCR amplified-mRNA in stimulated cells for IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-10 and GM-CSF compared to unstimulated cells. Changes in CD3δ, TNFα or IL-12 were not observed or quantitated. We present a novel aequorin-based application of bioluminescent technology to directly quantitate RT-PCR amplicons and to investigate the induction of human cytokine expression. Significant advantages of this sensitive bioluminescent method compared with radioactive methods are its abilities to quantitate amplicons in a PCR cycle range where linear detection is most robust and to analyze products in an automated, open-architecture microtiter plate format." @default.
• W2000495523 created "2016-06-24" @default.
• W2000495523 creator A5027427165 @default.
• W2000495523 creator A5050221150 @default.
• W2000495523 creator A5054690093 @default.
• W2000495523 creator A5077775717 @default.
• W2000495523 creator A5085639427 @default.
• W2000495523 date "1998-02-01" @default.
• W2000495523 modified "2023-10-18" @default.
• W2000495523 title "A flash-type bioluminescent immunoassay that is more sensitive than radioimaging: quantitative detection of cytokine cDNA in activated and resting human cells" @default.
• W2000495523 cites W1565758507 @default.
• W2000495523 cites W1903515437 @default.
• W2000495523 cites W1934151086 @default.
• W2000495523 cites W1978169208 @default.
• W2000495523 cites W1980151159 @default.
• W2000495523 cites W1988517220 @default.
• W2000495523 cites W1998045218 @default.
• W2000495523 cites W2012596159 @default.
• W2000495523 cites W2019257033 @default.
• W2000495523 cites W2019433479 @default.
• W2000495523 cites W2035792726 @default.
• W2000495523 cites W2039385640 @default.
• W2000495523 cites W2058809738 @default.
• W2000495523 cites W2078658984 @default.
• W2000495523 cites W2088661249 @default.
• W2000495523 cites W2093009120 @default.
• W2000495523 cites W2095339034 @default.
• W2000495523 cites W2098220678 @default.
• W2000495523 cites W2120321190 @default.
• W2000495523 cites W2149897099 @default.
• W2000495523 cites W2414755352 @default.
• W2000495523 cites W4245108619 @default.
• W2000495523 doi "https://doi.org/10.1016/s0022-1759(97)00190-7" @default.
• W2000495523 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9617832" @default.
• W2000495523 hasPublicationYear "1998" @default.
• W2000495523 type Work @default.
• W2000495523 sameAs 2000495523 @default.
• W2000495523 citedByCount "40" @default.
• W2000495523 countsByYear W20004955232014 @default.
• W2000495523 countsByYear W20004955232015 @default.
• W2000495523 crossrefType "journal-article" @default.
• W2000495523 hasAuthorship W2000495523A5027427165 @default.
• W2000495523 hasAuthorship W2000495523A5050221150 @default.
• W2000495523 hasAuthorship W2000495523A5054690093 @default.
• W2000495523 hasAuthorship W2000495523A5077775717 @default.
• W2000495523 hasAuthorship W2000495523A5085639427 @default.
• W2000495523 hasConcept C104317684 @default.
• W2000495523 hasConcept C137061746 @default.
• W2000495523 hasConcept C153911025 @default.
• W2000495523 hasConcept C159654299 @default.
• W2000495523 hasConcept C187882448 @default.
• W2000495523 hasConcept C202751555 @default.
• W2000495523 hasConcept C203014093 @default.
• W2000495523 hasConcept C2778690821 @default.
• W2000495523 hasConcept C2780420688 @default.
• W2000495523 hasConcept C40767141 @default.
• W2000495523 hasConcept C49105822 @default.
• W2000495523 hasConcept C5176359 @default.
• W2000495523 hasConcept C55493867 @default.
• W2000495523 hasConcept C79879829 @default.
• W2000495523 hasConcept C80933277 @default.
• W2000495523 hasConcept C8185291 @default.
• W2000495523 hasConcept C86803240 @default.
• W2000495523 hasConcept C95444343 @default.
• W2000495523 hasConceptScore W2000495523C104317684 @default.
• W2000495523 hasConceptScore W2000495523C137061746 @default.
• W2000495523 hasConceptScore W2000495523C153911025 @default.
• W2000495523 hasConceptScore W2000495523C159654299 @default.
• W2000495523 hasConceptScore W2000495523C187882448 @default.
• W2000495523 hasConceptScore W2000495523C202751555 @default.
• W2000495523 hasConceptScore W2000495523C203014093 @default.
• W2000495523 hasConceptScore W2000495523C2778690821 @default.
• W2000495523 hasConceptScore W2000495523C2780420688 @default.
• W2000495523 hasConceptScore W2000495523C40767141 @default.
• W2000495523 hasConceptScore W2000495523C49105822 @default.
• W2000495523 hasConceptScore W2000495523C5176359 @default.
• W2000495523 hasConceptScore W2000495523C55493867 @default.
• W2000495523 hasConceptScore W2000495523C79879829 @default.
• W2000495523 hasConceptScore W2000495523C80933277 @default.
• W2000495523 hasConceptScore W2000495523C8185291 @default.
• W2000495523 hasConceptScore W2000495523C86803240 @default.
• W2000495523 hasConceptScore W2000495523C95444343 @default.
• W2000495523 hasIssue "1-2" @default.
• W2000495523 hasLocation W20004955231 @default.
• W2000495523 hasLocation W20004955232 @default.
• W2000495523 hasOpenAccess W2000495523 @default.
• W2000495523 hasPrimaryLocation W20004955231 @default.
• W2000495523 hasRelatedWork W1506985229 @default.
• W2000495523 hasRelatedWork W1991426989 @default.
• W2000495523 hasRelatedWork W2014422602 @default.
• W2000495523 hasRelatedWork W2064571977 @default.
• W2000495523 hasRelatedWork W2073251801 @default.
• W2000495523 hasRelatedWork W2086773777 @default.
• W2000495523 hasRelatedWork W2096974951 @default.
• W2000495523 hasRelatedWork W2168724650 @default.
• W2000495523 hasRelatedWork W2468312763 @default.
• W2000495523 hasRelatedWork W2509013667 @default.
• W2000495523 hasVolume "211" @default.

• next