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- W2000496750 abstract "A high-performance liquid chromatographic (HPLC) assay for the identification and quantification of barbiturates in blood at therapeutic levels had been developed. An ODS-silica column is used with an eluent of 40% methanol at pH 8.5. The barbiturates are detected at 240 nm. The sample preparation procedure involves extraction of unfractionated blood (100 microliter) with hexane-diethyl ether (50 : 50, v/v) ang is very rapid. Talbutal is used as an internal standard. The method has been applied to the determination of five barbiturates (amylobarbitone, butobarbitone, cyclobarbitone, pentobarbitone and quinalbarbitone) in blood after therapeutic doses of the drugs. An application of the HPLC assay to forensic casework is demonstrated." @default.
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- W2000496750 date "1982-12-01" @default.
- W2000496750 modified "2023-09-24" @default.
- W2000496750 title "Analysis of barbiturates in blood by high-performance liquid chromatography" @default.
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- W2000496750 doi "https://doi.org/10.1016/s0378-4347(00)81783-1" @default.
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