FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000497731> ?p ?o ?g. }

• W2000497731 endingPage "395" @default.
• W2000497731 startingPage "395" @default.
• W2000497731 abstract "The expression of the cytoskeleton protein Keratin 18 (KRT18) starts at the onset of bovine blastocyst formation. KRT18 is solely expressed in the trophectoderm and can therefore be used as a marker for trophectodermal differentiation. In the present study, the expression of KRT18 was suppressed by RNA interference to probe its functional importance in bovine blastocyst formation. Microinjection of KRT18 double-stranded RNA into the cytoplasm of zygotes resulted in reduced KRT18 mRNA (76% reduction) and protein expression at the blastocyst stage and a lower developmental competence (41% reduction in the percentage of blastocyst formation) compared with non-injected and phosphate-buffered saline (PBS)-injected controls. KRT18 downregulation was associated with reduced mRNA expression of KRT8, the binding partner of KRT18, but had no effect on the expression of KRT19, CDH1 and DSP, other genes involved in intermediate filament and cytoskeleton formation. The results of the present study demonstrated that KRT18 knockdown in preimplantation embryos results in reduced blastocyst formation, but no further morphological aberrations were observed with regard to the biological function of KRT18. These observations could be due to the function of KRT18 being replaced by that of another gene, the surviving blastocysts expressing the minimum level of KRT18 required for normal blastocyst development or the possibility that further aberrations may occur later in development." @default.
• W2000497731 created "2016-06-24" @default.
• W2000497731 creator A5009402304 @default.
• W2000497731 creator A5020715943 @default.
• W2000497731 creator A5028471651 @default.
• W2000497731 creator A5036995219 @default.
• W2000497731 creator A5040221274 @default.
• W2000497731 creator A5041245667 @default.
• W2000497731 creator A5048600002 @default.
• W2000497731 creator A5048685893 @default.
• W2000497731 creator A5063436842 @default.
• W2000497731 creator A5089805368 @default.
• W2000497731 date "2010-01-01" @default.
• W2000497731 modified "2023-10-02" @default.
• W2000497731 title "Suppression of keratin 18 gene expression in bovine blastocysts by RNA interference" @default.
• W2000497731 cites W1647075334 @default.
• W2000497731 cites W1976347030 @default.
• W2000497731 cites W1978424801 @default.
• W2000497731 cites W1981752846 @default.
• W2000497731 cites W1986516415 @default.
• W2000497731 cites W1990402449 @default.
• W2000497731 cites W1991857980 @default.
• W2000497731 cites W2006586193 @default.
• W2000497731 cites W2007091840 @default.
• W2000497731 cites W2009965923 @default.
• W2000497731 cites W2019357379 @default.
• W2000497731 cites W2025142978 @default.
• W2000497731 cites W2043656927 @default.
• W2000497731 cites W2045508596 @default.
• W2000497731 cites W2048484534 @default.
• W2000497731 cites W2048690485 @default.
• W2000497731 cites W2055394838 @default.
• W2000497731 cites W2061298012 @default.
• W2000497731 cites W2076872166 @default.
• W2000497731 cites W2077653359 @default.
• W2000497731 cites W2080564653 @default.
• W2000497731 cites W2081504673 @default.
• W2000497731 cites W2087716264 @default.
• W2000497731 cites W2089616792 @default.
• W2000497731 cites W2090288014 @default.
• W2000497731 cites W2092180000 @default.
• W2000497731 cites W2102296508 @default.
• W2000497731 cites W2102298903 @default.
• W2000497731 cites W2107235611 @default.
• W2000497731 cites W2109763422 @default.
• W2000497731 cites W2109937387 @default.
• W2000497731 cites W2114406978 @default.
• W2000497731 cites W2114597181 @default.
• W2000497731 cites W2117979185 @default.
• W2000497731 cites W2124398372 @default.
• W2000497731 cites W2126582065 @default.
• W2000497731 cites W2135055501 @default.
• W2000497731 cites W2136597956 @default.
• W2000497731 cites W2140925807 @default.
• W2000497731 cites W2141157874 @default.
• W2000497731 cites W2143776806 @default.
• W2000497731 cites W2146138017 @default.
• W2000497731 cites W2151231857 @default.
• W2000497731 cites W2151344088 @default.
• W2000497731 cites W2185151129 @default.
• W2000497731 cites W2228792026 @default.
• W2000497731 cites W2404026649 @default.
• W2000497731 cites W3024152856 @default.
• W2000497731 cites W4239346882 @default.
• W2000497731 doi "https://doi.org/10.1071/rd09080" @default.
• W2000497731 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20047725" @default.
• W2000497731 hasPublicationYear "2010" @default.
• W2000497731 type Work @default.
• W2000497731 sameAs 2000497731 @default.
• W2000497731 citedByCount "12" @default.
• W2000497731 countsByYear W20004977312012 @default.
• W2000497731 countsByYear W20004977312015 @default.
• W2000497731 countsByYear W20004977312017 @default.
• W2000497731 countsByYear W20004977312019 @default.
• W2000497731 countsByYear W20004977312021 @default.
• W2000497731 countsByYear W20004977312022 @default.
• W2000497731 crossrefType "journal-article" @default.
• W2000497731 hasAuthorship W2000497731A5009402304 @default.
• W2000497731 hasAuthorship W2000497731A5020715943 @default.
• W2000497731 hasAuthorship W2000497731A5028471651 @default.
• W2000497731 hasAuthorship W2000497731A5036995219 @default.
• W2000497731 hasAuthorship W2000497731A5040221274 @default.
• W2000497731 hasAuthorship W2000497731A5041245667 @default.
• W2000497731 hasAuthorship W2000497731A5048600002 @default.
• W2000497731 hasAuthorship W2000497731A5048685893 @default.
• W2000497731 hasAuthorship W2000497731A5063436842 @default.
• W2000497731 hasAuthorship W2000497731A5089805368 @default.
• W2000497731 hasConcept C104317684 @default.
• W2000497731 hasConcept C16685009 @default.
• W2000497731 hasConcept C173396325 @default.
• W2000497731 hasConcept C185592680 @default.
• W2000497731 hasConcept C196843134 @default.
• W2000497731 hasConcept C2778177303 @default.
• W2000497731 hasConcept C54355233 @default.
• W2000497731 hasConcept C71924100 @default.
• W2000497731 hasConcept C86803240 @default.
• W2000497731 hasConcept C87073359 @default.
• W2000497731 hasConcept C95444343 @default.

• next