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• W2000498628 abstract "Highly uniform thin films of samples for matrix-assisted laser desorption/ionisation (MALDI) have been fabricated by depositing a saturated solution of ferulic acid onto a soda lime glass disc and crushing with polished aluminium, the films covering large areas of the substrate and having a thickness between 45–60 μm. The effects that different substrates and crushing materials as well as sample concentration and sample recrystallisation have on these films has been examined by scanning electron microscopy. Such films have been shown to have a lower threshold fluence for matrix ion detection than standard dried-droplet samples, the reduction being approximately 15% for three of the five matrices analysed. An explanation for this is proposed in terms of crushed samples possessing a greater average energy per unit volume coupled to them by the laser due to their improved surface uniformity. Furthermore, samples that are dried at refrigerated temperatures (∼ 2.25°C) are shown to have a much improved macroscopic uniformity over samples dried at room temperature. Refrigerated and crushed MALDI samples yield analyte ions with good spot-to-spot and pulse-to-pulse reproducibility and both preparation steps appear to improve the resolution of spectra obtained with a time-of-flight mass spectrometer." @default.
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• W2000498628 date "1996-11-01" @default.
• W2000498628 modified "2023-10-14" @default.
• W2000498628 title "Preparation of ‘near’ homogeneous samples for the analysis of matrix-assisted laser desorption/ionisation processes" @default.
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• W2000498628 doi "https://doi.org/10.1016/0169-4332(96)00530-2" @default.
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