Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000504595> ?p ?o ?g. }
Showing items 1 to 92 of
92
with 100 items per page.
- W2000504595 endingPage "2519" @default.
- W2000504595 startingPage "2515" @default.
- W2000504595 abstract "A beta-1,6-glucanase was purified to apparent homogeneity from a commercial yeast digestive enzyme prepared from Streptomyces rochei by a series of column chromatographies. The molecular mass of the purified enzyme was 60 kDa by SDS-PAGE. The purified enzyme had an optimum pH range from 4.0 to 6.0 and was stable in the same pH range. The enzyme was stable under 50 degrees C but lost almost all activity at 60 degrees C. The enzyme was specific to beta-1,6-glucan and had little activity towards beta-1,3-glucan and beta-1,4-glucan. When the beta-1,6-glucan was hydrolyzed with the purified enzyme for 5 h, the reaction products contained 20% glucose, 36% gentiobiose, and 44% other oligosaccharides, suggesting that the enzyme is an endo-type glucanase. When the purified enzyme was used for the digestion of the cell wall of Saccharomyces cerevisiae, cell-wall proteins covalently bound to the cell-wall glucan were recovered as soluble forms, suggesting that this enzyme is useful for analysis of yeast-cell wall proteins." @default.
- W2000504595 created "2016-06-24" @default.
- W2000504595 creator A5014426871 @default.
- W2000504595 creator A5090232193 @default.
- W2000504595 creator A5090846112 @default.
- W2000504595 date "2002-01-01" @default.
- W2000504595 modified "2023-10-09" @default.
- W2000504595 title "Purification and Characterization of β-1,6-Glucanase of<i>Streptomyces rochei</i>Application in the Study of Yeast Cell Wall Proteins" @default.
- W2000504595 cites W1957404662 @default.
- W2000504595 cites W1990593682 @default.
- W2000504595 cites W1996677209 @default.
- W2000504595 cites W2014732005 @default.
- W2000504595 cites W2063066968 @default.
- W2000504595 cites W2100837269 @default.
- W2000504595 cites W2107094644 @default.
- W2000504595 cites W2130316063 @default.
- W2000504595 cites W2136076378 @default.
- W2000504595 cites W2141324927 @default.
- W2000504595 cites W2145008199 @default.
- W2000504595 cites W2169333620 @default.
- W2000504595 cites W4250874261 @default.
- W2000504595 doi "https://doi.org/10.1271/bbb.66.2515" @default.
- W2000504595 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12507000" @default.
- W2000504595 hasPublicationYear "2002" @default.
- W2000504595 type Work @default.
- W2000504595 sameAs 2000504595 @default.
- W2000504595 citedByCount "6" @default.
- W2000504595 countsByYear W20005045952012 @default.
- W2000504595 countsByYear W20005045952016 @default.
- W2000504595 countsByYear W20005045952020 @default.
- W2000504595 countsByYear W20005045952022 @default.
- W2000504595 crossrefType "journal-article" @default.
- W2000504595 hasAuthorship W2000504595A5014426871 @default.
- W2000504595 hasAuthorship W2000504595A5090232193 @default.
- W2000504595 hasAuthorship W2000504595A5090846112 @default.
- W2000504595 hasBestOaLocation W20005045951 @default.
- W2000504595 hasConcept C125235067 @default.
- W2000504595 hasConcept C141603559 @default.
- W2000504595 hasConcept C181199279 @default.
- W2000504595 hasConcept C185592680 @default.
- W2000504595 hasConcept C2776123893 @default.
- W2000504595 hasConcept C2777576037 @default.
- W2000504595 hasConcept C2779222958 @default.
- W2000504595 hasConcept C2779530776 @default.
- W2000504595 hasConcept C2779920670 @default.
- W2000504595 hasConcept C28212737 @default.
- W2000504595 hasConcept C43617362 @default.
- W2000504595 hasConcept C523546767 @default.
- W2000504595 hasConcept C54355233 @default.
- W2000504595 hasConcept C55493867 @default.
- W2000504595 hasConcept C58121356 @default.
- W2000504595 hasConcept C86803240 @default.
- W2000504595 hasConcept C94412978 @default.
- W2000504595 hasConceptScore W2000504595C125235067 @default.
- W2000504595 hasConceptScore W2000504595C141603559 @default.
- W2000504595 hasConceptScore W2000504595C181199279 @default.
- W2000504595 hasConceptScore W2000504595C185592680 @default.
- W2000504595 hasConceptScore W2000504595C2776123893 @default.
- W2000504595 hasConceptScore W2000504595C2777576037 @default.
- W2000504595 hasConceptScore W2000504595C2779222958 @default.
- W2000504595 hasConceptScore W2000504595C2779530776 @default.
- W2000504595 hasConceptScore W2000504595C2779920670 @default.
- W2000504595 hasConceptScore W2000504595C28212737 @default.
- W2000504595 hasConceptScore W2000504595C43617362 @default.
- W2000504595 hasConceptScore W2000504595C523546767 @default.
- W2000504595 hasConceptScore W2000504595C54355233 @default.
- W2000504595 hasConceptScore W2000504595C55493867 @default.
- W2000504595 hasConceptScore W2000504595C58121356 @default.
- W2000504595 hasConceptScore W2000504595C86803240 @default.
- W2000504595 hasConceptScore W2000504595C94412978 @default.
- W2000504595 hasIssue "11" @default.
- W2000504595 hasLocation W20005045951 @default.
- W2000504595 hasLocation W20005045952 @default.
- W2000504595 hasOpenAccess W2000504595 @default.
- W2000504595 hasPrimaryLocation W20005045951 @default.
- W2000504595 hasRelatedWork W1966249491 @default.
- W2000504595 hasRelatedWork W1981217938 @default.
- W2000504595 hasRelatedWork W1987060735 @default.
- W2000504595 hasRelatedWork W2004914097 @default.
- W2000504595 hasRelatedWork W2017926045 @default.
- W2000504595 hasRelatedWork W2055655256 @default.
- W2000504595 hasRelatedWork W2082128449 @default.
- W2000504595 hasRelatedWork W2269389563 @default.
- W2000504595 hasRelatedWork W2298696461 @default.
- W2000504595 hasRelatedWork W4251014709 @default.
- W2000504595 hasVolume "66" @default.
- W2000504595 isParatext "false" @default.
- W2000504595 isRetracted "false" @default.
- W2000504595 magId "2000504595" @default.
- W2000504595 workType "article" @default.