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• W2000506255 abstract "Alternatively spliced human glutaredoxin (Grx1_as) cDNA was isolated from a neutrophil cDNA library, using a ³²P-labeled human glutaredoxin (Grx1) cDNA probe under non-stringent conditions. The sequence of Grx1_as cDNA indicated that the open reading frame of the gene was identical to the open reading frame of the previously reported first human glutaredoxin (Grx1) cDNA, but the 3′-untranslated region of Grx1_as was not homologous to Grx1 cDNA. Northern blot and RT-PCR analyses showed Grx1_as mRNA was expressed in normal human neutrophils and transformed cells including U937, HL-60, THP, and Jurkat cells. Cloning and sequencing of the genomic gene corresponding to Grx1_as cDNA showed that two different glutaredoxin cDNAs (Grx1_as and Grx1) were generated from the same genomic gene via alternative splicing. Origination of Grx1_as and Grx1 from the same gene was confirmed by chromosomal localization of the Grx1_as gene to chromosome 5q13, the same location where the Grx1 gene was localized previously. During screening of the Grx1_as genomic gene, two additional glutaredoxin pseudogenes were also isolated. Surprisingly, these pseudogenes contained 3′-untranslated regions that were nearly identical to the 3′-untranslated regions of Grx1_as, not Grx1, cDNA. Because 3′-untranslated regions may be important in stabilizing mRNAs, the effect of the two 3′-untranslated regions of Grx1 and Grx1_as on mRNA stability was investigated using luciferase reporter vectors with the 3′-untranslated regions. Luciferase activity was 2.6-fold greater in cells transfected with the reporter vector containing the 3′-untranslated region of Grx1_as cDNA compared with the 3′-untranslated region of Grx1 cDNA. These data indicate that Grx1_as cDNA is an alternatively spliced human Grx1 cDNA and that the Grx1_as 3′-untranslated region may have a role in stabilizing mRNA." @default.
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• W2000506255 date "2005-03-01" @default.
• W2000506255 modified "2023-10-18" @default.
• W2000506255 title "Cloning, Sequencing, and Characterization of Alternatively Spliced Glutaredoxin 1 cDNA and Its Genomic Gene" @default.
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• W2000506255 doi "https://doi.org/10.1074/jbc.m412450200" @default.
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