FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000508647> ?p ?o ?g. }

• W2000508647 endingPage "10630" @default.
• W2000508647 startingPage "10623" @default.
• W2000508647 abstract "DypB from Rhodococcus jostii RHA1 is a bacterial dye-decolorizing peroxidase (DyP) that oxidizes lignin and Mn(II). Three residues interact with the iron-bound solvent species in ferric DypB: Asn-246 and the conserved Asp-153 and Arg-244. Substitution of either Asp-153 or Asn-246 with alanine minimally affected the second order rate constant for Compound I formation (k(1) ∼ 10(5) M(-1)s(-1)) and the specificity constant (k(cat)/K(m)) for H(2)O(2). Even in the D153A/N246A double variant, these values were reduced less than 30-fold. However, these substitutions dramatically reduced the stability of Compound I (t(1/2) ∼ 0.13 s) as compared with the wild-type enzyme (540 s). By contrast, substitution of Arg-244 with leucine abolished the peroxidase activity, and heme iron of the variant showed a pH-dependent transition from high spin (pH 5) to low spin (pH 8.5). Two variants were designed to mimic the plant peroxidase active site: D153H, which was more than an order of magnitude less reactive with H(2)O(2), and N246H, which had no detectable peroxidase activity. X-ray crystallographic studies revealed that structural changes in the variants are confined to the distal heme environment. The data establish an essential role for Arg-244 in Compound I formation in DypB, possibly through charge stabilization and proton transfer. The principle roles of Asp-153 and Asn-246 appear to be in modulating the subsequent reactivity of Compound I. These results expand the range of residues known to catalyze Compound I formation in heme peroxidases." @default.
• W2000508647 created "2016-06-24" @default.
• W2000508647 creator A5003260841 @default.
• W2000508647 creator A5021302922 @default.
• W2000508647 creator A5037721636 @default.
• W2000508647 creator A5071277571 @default.
• W2000508647 creator A5077634475 @default.
• W2000508647 date "2012-03-01" @default.
• W2000508647 modified "2023-10-15" @default.
• W2000508647 title "Distal Heme Pocket Residues of B-type Dye-decolorizing Peroxidase" @default.
• W2000508647 cites W1539796472 @default.
• W2000508647 cites W1571455001 @default.
• W2000508647 cites W1803620352 @default.
• W2000508647 cites W1967821193 @default.
• W2000508647 cites W1990205765 @default.
• W2000508647 cites W1990392212 @default.
• W2000508647 cites W1995002017 @default.
• W2000508647 cites W1996377626 @default.
• W2000508647 cites W1999898395 @default.
• W2000508647 cites W2001641653 @default.
• W2000508647 cites W2008610282 @default.
• W2000508647 cites W2014944909 @default.
• W2000508647 cites W2038529067 @default.
• W2000508647 cites W2038840577 @default.
• W2000508647 cites W2048459982 @default.
• W2000508647 cites W2059307992 @default.
• W2000508647 cites W2074336931 @default.
• W2000508647 cites W2086477248 @default.
• W2000508647 cites W2090933843 @default.
• W2000508647 cites W2092434707 @default.
• W2000508647 cites W2108586333 @default.
• W2000508647 cites W2124026197 @default.
• W2000508647 cites W2124124969 @default.
• W2000508647 cites W2138416619 @default.
• W2000508647 cites W2149293626 @default.
• W2000508647 cites W2150638266 @default.
• W2000508647 cites W2159529068 @default.
• W2000508647 cites W2159746325 @default.
• W2000508647 cites W2165898505 @default.
• W2000508647 cites W2321627128 @default.
• W2000508647 doi "https://doi.org/10.1074/jbc.m111.332171" @default.
• W2000508647 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3323036" @default.
• W2000508647 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22308037" @default.
• W2000508647 hasPublicationYear "2012" @default.
• W2000508647 type Work @default.
• W2000508647 sameAs 2000508647 @default.
• W2000508647 citedByCount "90" @default.
• W2000508647 countsByYear W20005086472012 @default.
• W2000508647 countsByYear W20005086472013 @default.
• W2000508647 countsByYear W20005086472014 @default.
• W2000508647 countsByYear W20005086472015 @default.
• W2000508647 countsByYear W20005086472016 @default.
• W2000508647 countsByYear W20005086472017 @default.
• W2000508647 countsByYear W20005086472018 @default.
• W2000508647 countsByYear W20005086472019 @default.
• W2000508647 countsByYear W20005086472020 @default.
• W2000508647 countsByYear W20005086472021 @default.
• W2000508647 countsByYear W20005086472022 @default.
• W2000508647 countsByYear W20005086472023 @default.
• W2000508647 crossrefType "journal-article" @default.
• W2000508647 hasAuthorship W2000508647A5003260841 @default.
• W2000508647 hasAuthorship W2000508647A5021302922 @default.
• W2000508647 hasAuthorship W2000508647A5037721636 @default.
• W2000508647 hasAuthorship W2000508647A5071277571 @default.
• W2000508647 hasAuthorship W2000508647A5077634475 @default.
• W2000508647 hasBestOaLocation W20005086471 @default.
• W2000508647 hasConcept C104292427 @default.
• W2000508647 hasConcept C104317684 @default.
• W2000508647 hasConcept C133166286 @default.
• W2000508647 hasConcept C142724271 @default.
• W2000508647 hasConcept C162008176 @default.
• W2000508647 hasConcept C179104552 @default.
• W2000508647 hasConcept C181199279 @default.
• W2000508647 hasConcept C185592680 @default.
• W2000508647 hasConcept C204787440 @default.
• W2000508647 hasConcept C2776217839 @default.
• W2000508647 hasConcept C2776910235 @default.
• W2000508647 hasConcept C2780471494 @default.
• W2000508647 hasConcept C2780534640 @default.
• W2000508647 hasConcept C41183919 @default.
• W2000508647 hasConcept C55493867 @default.
• W2000508647 hasConcept C71240020 @default.
• W2000508647 hasConcept C71924100 @default.
• W2000508647 hasConcept C7927669 @default.
• W2000508647 hasConcept C8010536 @default.
• W2000508647 hasConceptScore W2000508647C104292427 @default.
• W2000508647 hasConceptScore W2000508647C104317684 @default.
• W2000508647 hasConceptScore W2000508647C133166286 @default.
• W2000508647 hasConceptScore W2000508647C142724271 @default.
• W2000508647 hasConceptScore W2000508647C162008176 @default.
• W2000508647 hasConceptScore W2000508647C179104552 @default.
• W2000508647 hasConceptScore W2000508647C181199279 @default.
• W2000508647 hasConceptScore W2000508647C185592680 @default.
• W2000508647 hasConceptScore W2000508647C204787440 @default.
• W2000508647 hasConceptScore W2000508647C2776217839 @default.
• W2000508647 hasConceptScore W2000508647C2776910235 @default.
• W2000508647 hasConceptScore W2000508647C2780471494 @default.
• W2000508647 hasConceptScore W2000508647C2780534640 @default.

• next