FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000509889> ?p ?o ?g. }

• W2000509889 endingPage "668" @default.
• W2000509889 startingPage "659" @default.
• W2000509889 abstract "Snake venoms contain a complex mixture of polypeptides that modulate prey homeostatic mechanisms through highly specific and targeted interactions. In this study we have identified and characterised cystatin-like cysteine-protease inhibitors from elapid snake venoms for the first time. Novel cystatin sequences were cloned from 12 of 13 elapid snake venom glands and the protein was detected, albeit at very low levels, in a total of 22 venoms. One highly conserved isoform, which displayed close sequence identity with family 2 cystatins, was detected in each elapid snake. Crude Austrelaps superbus (Australian lowland copperhead) snake venom inhibited papain, and a recombinant form of A. superbus cystatin inhibited cathepsin L ≅ papain > cathepsin B, with no inhibition observed for calpain or legumain. While snake venom cystatins have truncated N-termini, sequence alignment and structural modelling suggested that the evolutionarily conserved Gly-11 of family 2 cystatins, essential for cysteine protease inhibition, is conserved in snake venom cystatins as Gly-3. This was confirmed by mutagenesis at the Gly-3 site, which increased the dissociation constant for papain by 10(4)-fold. These data demonstrate that elapid snake venom cystatins are novel members of the type 2 family. The widespread, low level expression of type 2 cystatins in snake venom, as well as the presence of only one highly conserved isoform in each species, imply essential housekeeping or regulatory roles for these proteins." @default.
• W2000509889 created "2016-06-24" @default.
• W2000509889 creator A5010372263 @default.
• W2000509889 creator A5033108028 @default.
• W2000509889 creator A5064622840 @default.
• W2000509889 creator A5065197081 @default.
• W2000509889 creator A5067364038 @default.
• W2000509889 creator A5084003680 @default.
• W2000509889 creator A5086310893 @default.
• W2000509889 date "2011-04-01" @default.
• W2000509889 modified "2023-09-23" @default.
• W2000509889 title "Cloning and characterisation of novel cystatins from elapid snake venom glands" @default.
• W2000509889 cites W1484675846 @default.
• W2000509889 cites W1547251309 @default.
• W2000509889 cites W1737088483 @default.
• W2000509889 cites W1766974853 @default.
• W2000509889 cites W1788679618 @default.
• W2000509889 cites W1815390460 @default.
• W2000509889 cites W1862656502 @default.
• W2000509889 cites W1964589079 @default.
• W2000509889 cites W1975050432 @default.
• W2000509889 cites W1981733318 @default.
• W2000509889 cites W1985211089 @default.
• W2000509889 cites W1997280530 @default.
• W2000509889 cites W1998232583 @default.
• W2000509889 cites W2001518436 @default.
• W2000509889 cites W2002075122 @default.
• W2000509889 cites W2004709114 @default.
• W2000509889 cites W2008964357 @default.
• W2000509889 cites W2015642465 @default.
• W2000509889 cites W2020315414 @default.
• W2000509889 cites W2034978054 @default.
• W2000509889 cites W2041061923 @default.
• W2000509889 cites W2041905742 @default.
• W2000509889 cites W2043071886 @default.
• W2000509889 cites W2047919626 @default.
• W2000509889 cites W2050997138 @default.
• W2000509889 cites W2051906491 @default.
• W2000509889 cites W2054672987 @default.
• W2000509889 cites W2059091801 @default.
• W2000509889 cites W2060059149 @default.
• W2000509889 cites W2061741304 @default.
• W2000509889 cites W2064472003 @default.
• W2000509889 cites W2064964414 @default.
• W2000509889 cites W2066352013 @default.
• W2000509889 cites W2068881539 @default.
• W2000509889 cites W2075682016 @default.
• W2000509889 cites W2077593763 @default.
• W2000509889 cites W2077724681 @default.
• W2000509889 cites W2082618568 @default.
• W2000509889 cites W2084532510 @default.
• W2000509889 cites W2087642074 @default.
• W2000509889 cites W2095541332 @default.
• W2000509889 cites W2097912624 @default.
• W2000509889 cites W2107618620 @default.
• W2000509889 cites W2114027069 @default.
• W2000509889 cites W2140248001 @default.
• W2000509889 cites W2147914469 @default.
• W2000509889 cites W2148505321 @default.
• W2000509889 cites W2152301430 @default.
• W2000509889 cites W2158783623 @default.
• W2000509889 cites W2219598379 @default.
• W2000509889 cites W2221094965 @default.
• W2000509889 cites W2284687780 @default.
• W2000509889 cites W2394668306 @default.
• W2000509889 cites W3146308990 @default.
• W2000509889 cites W2025476517 @default.
• W2000509889 doi "https://doi.org/10.1016/j.biochi.2010.12.008" @default.
• W2000509889 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21172403" @default.
• W2000509889 hasPublicationYear "2011" @default.
• W2000509889 type Work @default.
• W2000509889 sameAs 2000509889 @default.
• W2000509889 citedByCount "20" @default.
• W2000509889 countsByYear W20005098892012 @default.
• W2000509889 countsByYear W20005098892013 @default.
• W2000509889 countsByYear W20005098892014 @default.
• W2000509889 countsByYear W20005098892015 @default.
• W2000509889 countsByYear W20005098892016 @default.
• W2000509889 countsByYear W20005098892017 @default.
• W2000509889 countsByYear W20005098892018 @default.
• W2000509889 countsByYear W20005098892020 @default.
• W2000509889 countsByYear W20005098892022 @default.
• W2000509889 crossrefType "journal-article" @default.
• W2000509889 hasAuthorship W2000509889A5010372263 @default.
• W2000509889 hasAuthorship W2000509889A5033108028 @default.
• W2000509889 hasAuthorship W2000509889A5064622840 @default.
• W2000509889 hasAuthorship W2000509889A5065197081 @default.
• W2000509889 hasAuthorship W2000509889A5067364038 @default.
• W2000509889 hasAuthorship W2000509889A5084003680 @default.
• W2000509889 hasAuthorship W2000509889A5086310893 @default.
• W2000509889 hasConcept C153911025 @default.
• W2000509889 hasConcept C159641895 @default.
• W2000509889 hasConcept C181199279 @default.
• W2000509889 hasConcept C2776725428 @default.
• W2000509889 hasConcept C2777009914 @default.
• W2000509889 hasConcept C2779448229 @default.
• W2000509889 hasConcept C2779548226 @default.
• W2000509889 hasConcept C2779832904 @default.

• next