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- W2000510009 abstract "The synthesis of ribonucleic acid strands by RNA polymerase enzymes has long been known to initiate specifically at well-defined promoter sequences.1 For example, the T7 RNA polymerase enzyme initiates RNA strand synthesis with a pppG residue immediately downstream from a conserved ~17-base-pair duplex DNA sequence.2 This fact has been used in the “runoff transcription” method for laboratory synthesis of sequence-defined oligoribonucleotides, in which DNA strands which contain the double-stranded promoter at one end serve as templates for RNA synthesis.3" @default.
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- W2000510009 date "1995-07-01" @default.
- W2000510009 modified "2023-10-05" @default.
- W2000510009 title "Rolling-Circle RNA Synthesis: Circular Oligonucleotides as Efficient Substrates for T7 RNA Polymerase" @default.
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- W2000510009 doi "https://doi.org/10.1021/ja00134a032" @default.
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