Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000515250> ?p ?o ?g. }
- W2000515250 abstract "The integral membrane-bound Nrf1 transcription factor fulfils important functions in maintaining cellular homeostasis and organ integrity, but how it is controlled vectorially is unknown. Herein, creative use of Gal4-based reporter assays with protease protection assays (GRAPPA) and double fluorescence protease protection (dFPP), reveals that the membrane-topogenic vectorial behaviour of Nrf1 dictates its post-translational modification and transactivation activity. Nrf1 is integrated within endoplasmic reticulum (ER) membranes through its NHB1-associated TM1 in cooperation with other semihydrophobic amphipathic regions. The transactivation domains (TADs) of Nrf1, including its Asn/Ser/Thr-rich (NST) glycodomain, are transiently translocated into the ER lumen, where it is glycosylated in the presence of glucose to become a 120-kDa isoform. Thereafter, the NST-adjoining TADs are partially repartitioned out of membranes into the cyto/nucleoplasmic side, where Nrf1 is subject to deglycosylation and/or proteolysis to generate 95-kDa and 85-kDa isoforms. Therefore, the vectorial process of Nrf1 controls its target gene expression." @default.
- W2000515250 created "2016-06-24" @default.
- W2000515250 creator A5019212356 @default.
- W2000515250 creator A5046024649 @default.
- W2000515250 date "2013-06-18" @default.
- W2000515250 modified "2023-10-16" @default.
- W2000515250 title "The membrane-topogenic vectorial behaviour of Nrf1 controls its post-translational modification and transactivation activity" @default.
- W2000515250 cites W1549119424 @default.
- W2000515250 cites W1828993336 @default.
- W2000515250 cites W1966094020 @default.
- W2000515250 cites W1969727475 @default.
- W2000515250 cites W1969775817 @default.
- W2000515250 cites W1970500252 @default.
- W2000515250 cites W1973595140 @default.
- W2000515250 cites W1974465939 @default.
- W2000515250 cites W1974525592 @default.
- W2000515250 cites W1976882019 @default.
- W2000515250 cites W1979582828 @default.
- W2000515250 cites W1983036561 @default.
- W2000515250 cites W1990699073 @default.
- W2000515250 cites W1991265255 @default.
- W2000515250 cites W1991547229 @default.
- W2000515250 cites W2001094165 @default.
- W2000515250 cites W2003058284 @default.
- W2000515250 cites W2003384075 @default.
- W2000515250 cites W2004465970 @default.
- W2000515250 cites W2007143913 @default.
- W2000515250 cites W2010928027 @default.
- W2000515250 cites W2011124655 @default.
- W2000515250 cites W2016737119 @default.
- W2000515250 cites W2019313414 @default.
- W2000515250 cites W2027085316 @default.
- W2000515250 cites W2028553942 @default.
- W2000515250 cites W2028854226 @default.
- W2000515250 cites W2032169043 @default.
- W2000515250 cites W2033166053 @default.
- W2000515250 cites W2033215389 @default.
- W2000515250 cites W2033961874 @default.
- W2000515250 cites W2038210364 @default.
- W2000515250 cites W2038528121 @default.
- W2000515250 cites W2043619696 @default.
- W2000515250 cites W2050072642 @default.
- W2000515250 cites W2051641469 @default.
- W2000515250 cites W2054827827 @default.
- W2000515250 cites W2054907863 @default.
- W2000515250 cites W2056651186 @default.
- W2000515250 cites W2063097466 @default.
- W2000515250 cites W2065544945 @default.
- W2000515250 cites W2069895265 @default.
- W2000515250 cites W2069979286 @default.
- W2000515250 cites W2077643007 @default.
- W2000515250 cites W2078528430 @default.
- W2000515250 cites W2079415210 @default.
- W2000515250 cites W2081210689 @default.
- W2000515250 cites W2088487788 @default.
- W2000515250 cites W2102839649 @default.
- W2000515250 cites W2103061779 @default.
- W2000515250 cites W2106931266 @default.
- W2000515250 cites W2107996007 @default.
- W2000515250 cites W2108795738 @default.
- W2000515250 cites W2111679957 @default.
- W2000515250 cites W2112436307 @default.
- W2000515250 cites W2117986250 @default.
- W2000515250 cites W2122040451 @default.
- W2000515250 cites W2123565061 @default.
- W2000515250 cites W2126118567 @default.
- W2000515250 cites W2126248794 @default.
- W2000515250 cites W2127639686 @default.
- W2000515250 cites W2133529176 @default.
- W2000515250 cites W2133631756 @default.
- W2000515250 cites W2135646750 @default.
- W2000515250 cites W2136558879 @default.
- W2000515250 cites W2140422307 @default.
- W2000515250 cites W2141626933 @default.
- W2000515250 cites W2148606044 @default.
- W2000515250 cites W2151762765 @default.
- W2000515250 cites W2152542101 @default.
- W2000515250 cites W2154517085 @default.
- W2000515250 cites W2158982890 @default.
- W2000515250 cites W2163292409 @default.
- W2000515250 cites W2170443407 @default.
- W2000515250 cites W2171355310 @default.
- W2000515250 cites W2246719283 @default.
- W2000515250 doi "https://doi.org/10.1038/srep02006" @default.
- W2000515250 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3684815" @default.
- W2000515250 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23774320" @default.
- W2000515250 hasPublicationYear "2013" @default.
- W2000515250 type Work @default.
- W2000515250 sameAs 2000515250 @default.
- W2000515250 citedByCount "34" @default.
- W2000515250 countsByYear W20005152502014 @default.
- W2000515250 countsByYear W20005152502015 @default.
- W2000515250 countsByYear W20005152502016 @default.
- W2000515250 countsByYear W20005152502017 @default.
- W2000515250 countsByYear W20005152502018 @default.
- W2000515250 countsByYear W20005152502019 @default.
- W2000515250 countsByYear W20005152502020 @default.
- W2000515250 countsByYear W20005152502021 @default.
- W2000515250 countsByYear W20005152502022 @default.
- W2000515250 countsByYear W20005152502023 @default.