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• W2000516301 abstract "Scanning electrochemical microscopy (SECM) with enzyme immunoassay was applied to detect the cancer related antigen CA15-3 (Ag). In this method, CA15-3 was concentrated and immobilized on a plane substrate via a sandwich method employing two corresponding antibody (Ab captured on a streptavidin-coated substrate and Ab* labeled with horseradish peroxidase (HRP)). In the presence of hydroquinone (H2Q) and H2O2, HRP on the complex of CA15-3 with Ab and Ab*, Ab–Ag–Ab*, converted H2Q to the electroactive product benzoquinone (BQ) through the HRP-catalyzed reaction. The reduction current of BQ generated by the HRP-catalyzed reaction corresponding to the amount of CA15-3 was monitored and imaged by SECM. With a view of improving the sensitivity of SECM-enzyme immunoassay to meet our objective, Ab–Ag–Ab* was concentrated on the substrate via a microcell. The detection limit of this method was 2.5 U/mL for CA15-3." @default.
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• W2000516301 date "2006-02-01" @default.
• W2000516301 modified "2023-09-23" @default.
• W2000516301 title "Scanning electrochemical microscopy with enzyme immunoassay of the cancer-related antigen CA15-3" @default.
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• W2000516301 doi "https://doi.org/10.1016/j.aca.2005.11.032" @default.
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