FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000524346> ?p ?o ?g. }

• W2000524346 endingPage "111" @default.
• W2000524346 startingPage "105" @default.
• W2000524346 abstract "Optimal gene electrotransfer (GET) requires a compromise between maximum transgene expression and minimal tissue damage. GET in skeletal muscle can be improved by pretreatment with hyaluronidase which contributes to maximize transgene uptake and expression. Nevertheless, tissue damage remains severe close to the electrodes, with a concomitant loss of GET efficiency. Here we analyze the role of pH in tissue damage in GET protocols through in vivo modeling using a transparent chamber implanted into the dorsal skinfold of a mouse (DSC) and intravital microscopy, and in silico modeling using the Poisson–Nernst–Planck equations for ion transport. DSC intravital microscopy reveals the existence of pH fronts emerging from both electrodes and that these fronts are immediate and substantial thus giving rise to tissue necrosis. Theoretical modeling confirms experimental measurements and shows that in GET protocols whether with or without hyaluronidase pretreatment, pH fronts are the principal cause of muscle damage near the electrodes. It also predicts that an optimal efficiency in GET protocols, that is a compromise between obtaining maximum electroporated area and minimal tissue damage, is achieved when the electric field applied is near 183 V/cm in a GET protocol and 158 V/cm in a hyaluronidase + GET protocol." @default.
• W2000524346 created "2016-06-24" @default.
• W2000524346 creator A5017088440 @default.
• W2000524346 creator A5026746726 @default.
• W2000524346 creator A5028180841 @default.
• W2000524346 creator A5046545518 @default.
• W2000524346 creator A5047832923 @default.
• W2000524346 creator A5049072655 @default.
• W2000524346 creator A5060159212 @default.
• W2000524346 creator A5079098472 @default.
• W2000524346 date "2014-12-01" @default.
• W2000524346 modified "2023-09-27" @default.
• W2000524346 title "Tissue damage modeling in gene electrotransfer: The role of pH" @default.
• W2000524346 cites W1490324405 @default.
• W2000524346 cites W1904722209 @default.
• W2000524346 cites W1917091625 @default.
• W2000524346 cites W1963637896 @default.
• W2000524346 cites W1980980540 @default.
• W2000524346 cites W1984023770 @default.
• W2000524346 cites W1988230026 @default.
• W2000524346 cites W1992687839 @default.
• W2000524346 cites W2008110015 @default.
• W2000524346 cites W2019410912 @default.
• W2000524346 cites W2021312203 @default.
• W2000524346 cites W2026296992 @default.
• W2000524346 cites W2026761507 @default.
• W2000524346 cites W2032180343 @default.
• W2000524346 cites W2032783816 @default.
• W2000524346 cites W2035551088 @default.
• W2000524346 cites W2044198294 @default.
• W2000524346 cites W2044469114 @default.
• W2000524346 cites W2054357432 @default.
• W2000524346 cites W2087960497 @default.
• W2000524346 cites W2094626068 @default.
• W2000524346 cites W2103393861 @default.
• W2000524346 cites W2115049251 @default.
• W2000524346 cites W2118168322 @default.
• W2000524346 cites W2119630670 @default.
• W2000524346 cites W2127466034 @default.
• W2000524346 cites W2161332286 @default.
• W2000524346 cites W2168181497 @default.
• W2000524346 cites W4254021855 @default.
• W2000524346 doi "https://doi.org/10.1016/j.bioelechem.2014.05.001" @default.
• W2000524346 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24925861" @default.
• W2000524346 hasPublicationYear "2014" @default.
• W2000524346 type Work @default.
• W2000524346 sameAs 2000524346 @default.
• W2000524346 citedByCount "37" @default.
• W2000524346 countsByYear W20005243462014 @default.
• W2000524346 countsByYear W20005243462015 @default.
• W2000524346 countsByYear W20005243462016 @default.
• W2000524346 countsByYear W20005243462017 @default.
• W2000524346 countsByYear W20005243462018 @default.
• W2000524346 countsByYear W20005243462019 @default.
• W2000524346 countsByYear W20005243462020 @default.
• W2000524346 countsByYear W20005243462021 @default.
• W2000524346 countsByYear W20005243462022 @default.
• W2000524346 crossrefType "journal-article" @default.
• W2000524346 hasAuthorship W2000524346A5017088440 @default.
• W2000524346 hasAuthorship W2000524346A5026746726 @default.
• W2000524346 hasAuthorship W2000524346A5028180841 @default.
• W2000524346 hasAuthorship W2000524346A5046545518 @default.
• W2000524346 hasAuthorship W2000524346A5047832923 @default.
• W2000524346 hasAuthorship W2000524346A5049072655 @default.
• W2000524346 hasAuthorship W2000524346A5060159212 @default.
• W2000524346 hasAuthorship W2000524346A5079098472 @default.
• W2000524346 hasConcept C104317684 @default.
• W2000524346 hasConcept C12554922 @default.
• W2000524346 hasConcept C136229726 @default.
• W2000524346 hasConcept C185592680 @default.
• W2000524346 hasConcept C192562407 @default.
• W2000524346 hasConcept C207001950 @default.
• W2000524346 hasConcept C2775905019 @default.
• W2000524346 hasConcept C2859252 @default.
• W2000524346 hasConcept C54355233 @default.
• W2000524346 hasConcept C55493867 @default.
• W2000524346 hasConcept C71924100 @default.
• W2000524346 hasConcept C86803240 @default.
• W2000524346 hasConceptScore W2000524346C104317684 @default.
• W2000524346 hasConceptScore W2000524346C12554922 @default.
• W2000524346 hasConceptScore W2000524346C136229726 @default.
• W2000524346 hasConceptScore W2000524346C185592680 @default.
• W2000524346 hasConceptScore W2000524346C192562407 @default.
• W2000524346 hasConceptScore W2000524346C207001950 @default.
• W2000524346 hasConceptScore W2000524346C2775905019 @default.
• W2000524346 hasConceptScore W2000524346C2859252 @default.
• W2000524346 hasConceptScore W2000524346C54355233 @default.
• W2000524346 hasConceptScore W2000524346C55493867 @default.
• W2000524346 hasConceptScore W2000524346C71924100 @default.
• W2000524346 hasConceptScore W2000524346C86803240 @default.
• W2000524346 hasFunder F4320320268 @default.
• W2000524346 hasFunder F4320321594 @default.
• W2000524346 hasFunder F4320321664 @default.
• W2000524346 hasFunder F4320323257 @default.
• W2000524346 hasLocation W20005243461 @default.
• W2000524346 hasLocation W20005243462 @default.
• W2000524346 hasOpenAccess W2000524346 @default.
• W2000524346 hasPrimaryLocation W20005243461 @default.

• next