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- W2000524665 abstract "In Schwann cells, connexin 32 (Cx32) can oligomerize to form intracellular gap junction channels facilitating a shorter pathway for metabolite diffusion across the layers of the myelin sheath. The mechanisms of Cx32 intracellular channel regulation have not been clearly defined. However, Ca(2+), pH, and the phosphorylation state can regulate Cx32 gap junction channels, in addition to the direct interaction of protein partners with the carboxyl-terminal (CT) domain. In this study, we used different biophysical methods to determine the structure and characterize the interaction of the Cx32CT domain with the protein partners synapse-associated protein 97 (SAP97) and calmodulin (CaM). Our results revealed that the Cx32CT is an intrinsically disordered protein that becomes α-helical upon binding CaM. We identified the GUK domain as the minimal SAP97 region necessary for the Cx32CT interaction. The Cx32CT residues affected by the binding of CaM and the SAP97 GUK domain were determined as well as the dissociation constants for these interactions. We characterized three Cx32CT Charcot-Marie-Tooth disease mutants (R219H, R230C, and F235C) and identified that whereas they all formed functional channels, they all showed reduced binding affinity for SAP97 and CaM. Additionally, we report that in RT4-D6P2T rat schwannoma cells, Cx32 is differentially phosphorylated and exists in a complex with SAP97 and CaM. Our studies support the importance of protein-protein interactions in the regulation of Cx32 gap junction channels and myelin homeostasis." @default.
- W2000524665 created "2016-06-24" @default.
- W2000524665 creator A5051575971 @default.
- W2000524665 creator A5069294633 @default.
- W2000524665 creator A5070214965 @default.
- W2000524665 date "2012-08-01" @default.
- W2000524665 modified "2023-10-01" @default.
- W2000524665 title "Characterization of the Structure and Intermolecular Interactions between the Connexin 32 Carboxyl-terminal Domain and the Protein Partners Synapse-associated Protein 97 and Calmodulin" @default.
- W2000524665 cites W1516359509 @default.
- W2000524665 cites W1599345755 @default.
- W2000524665 cites W1601691609 @default.
- W2000524665 cites W1831581272 @default.
- W2000524665 cites W1834582800 @default.
- W2000524665 cites W1869363046 @default.
- W2000524665 cites W1876704297 @default.
- W2000524665 cites W1914765280 @default.
- W2000524665 cites W1966232883 @default.
- W2000524665 cites W1977153441 @default.
- W2000524665 cites W1984647008 @default.
- W2000524665 cites W1988832410 @default.
- W2000524665 cites W1992162703 @default.
- W2000524665 cites W1995174583 @default.
- W2000524665 cites W1995841153 @default.
- W2000524665 cites W1997816893 @default.
- W2000524665 cites W1999752453 @default.
- W2000524665 cites W2003008447 @default.
- W2000524665 cites W2003561135 @default.
- W2000524665 cites W2005818291 @default.
- W2000524665 cites W2010058764 @default.
- W2000524665 cites W2012841879 @default.
- W2000524665 cites W2013054583 @default.
- W2000524665 cites W2016151985 @default.
- W2000524665 cites W2022529986 @default.
- W2000524665 cites W2023169392 @default.
- W2000524665 cites W2024142216 @default.
- W2000524665 cites W2025878297 @default.
- W2000524665 cites W2026079063 @default.
- W2000524665 cites W2029378125 @default.
- W2000524665 cites W2029946033 @default.
- W2000524665 cites W2035491839 @default.
- W2000524665 cites W2036238031 @default.
- W2000524665 cites W2047669413 @default.
- W2000524665 cites W2051485189 @default.
- W2000524665 cites W2052606462 @default.
- W2000524665 cites W2053989248 @default.
- W2000524665 cites W2068227670 @default.
- W2000524665 cites W2068846597 @default.
- W2000524665 cites W2069753288 @default.
- W2000524665 cites W2069807165 @default.
- W2000524665 cites W2074437085 @default.
- W2000524665 cites W2074706630 @default.
- W2000524665 cites W2080202616 @default.
- W2000524665 cites W2082176639 @default.
- W2000524665 cites W2082553994 @default.
- W2000524665 cites W2083786873 @default.
- W2000524665 cites W2099305001 @default.
- W2000524665 cites W2099684738 @default.
- W2000524665 cites W2102883376 @default.
- W2000524665 cites W2109271830 @default.
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- W2000524665 cites W2142427933 @default.
- W2000524665 cites W2143868289 @default.
- W2000524665 cites W2146046916 @default.
- W2000524665 cites W2154080115 @default.
- W2000524665 cites W2154262751 @default.
- W2000524665 cites W2159395285 @default.
- W2000524665 cites W2163709463 @default.
- W2000524665 cites W2164570717 @default.
- W2000524665 cites W2164655870 @default.
- W2000524665 cites W2166901250 @default.
- W2000524665 cites W2169090805 @default.
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- W2000524665 doi "https://doi.org/10.1074/jbc.m112.382572" @default.
- W2000524665 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3431650" @default.
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- W2000524665 hasPublicationYear "2012" @default.
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