FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000524912> ?p ?o ?g. }

• W2000524912 endingPage "e0117508" @default.
• W2000524912 startingPage "e0117508" @default.
• W2000524912 abstract "Given the emerging evidence of an association between periodontal infections and systemic conditions, the search for specific methods to detect the presence of P. gingivalis, a principal etiologic agent in chronic periodontitis, is of high importance. The aim of this study was to characterize antibodies raised against purified P. gingivalis HmuY protein and selected epitopes of the HmuY molecule. Since other periodontopathogens produce homologs of HmuY, we also aimed to characterize responses of antibodies raised against the HmuY protein or its epitopes to the closest homologous proteins from Prevotella intermedia and Tannerella forsythia. Rabbits were immunized with purified HmuY protein or three synthetic, KLH-conjugated peptides, derived from the P. gingivalis HmuY protein. The reactivity of anti-HmuY antibodies with purified proteins or bacteria was determined using Western blotting and ELISA assay. First, we found homologs of P. gingivalis HmuY in P. intermedia (PinO and PinA proteins) and T. forsythia (Tfo protein) and identified corrected nucleotide and amino acid sequences of Tfo. All proteins were overexpressed in E. coli and purified using ion-exchange chromatography, hydrophobic chromatography and gel filtration. We demonstrated that antibodies raised against P. gingivalis HmuY are highly specific to purified HmuY protein and HmuY attached to P. gingivalis cells. No reactivity between P. intermedia and T. forsythia or between purified HmuY homologs from these bacteria and anti-HmuY antibodies was detected. The results obtained in this study demonstrate that P. gingivalis HmuY protein may serve as an antigen for specific determination of serum antibodies raised against this bacterium." @default.
• W2000524912 created "2016-06-24" @default.
• W2000524912 creator A5011897079 @default.
• W2000524912 creator A5044938537 @default.
• W2000524912 creator A5061127730 @default.
• W2000524912 creator A5082389527 @default.
• W2000524912 creator A5086095536 @default.
• W2000524912 date "2015-02-06" @default.
• W2000524912 modified "2023-10-16" @default.
• W2000524912 title "Anti-HmuY Antibodies Specifically Recognize Porphyromonas gingivalis HmuY Protein but Not Homologous Proteins in Other Periodontopathogens" @default.
• W2000524912 cites W1510009214 @default.
• W2000524912 cites W1580533751 @default.
• W2000524912 cites W1855914786 @default.
• W2000524912 cites W1964394911 @default.
• W2000524912 cites W1970283377 @default.
• W2000524912 cites W1971501638 @default.
• W2000524912 cites W1976515086 @default.
• W2000524912 cites W1985573972 @default.
• W2000524912 cites W1990766256 @default.
• W2000524912 cites W1993426964 @default.
• W2000524912 cites W1997725099 @default.
• W2000524912 cites W2000749448 @default.
• W2000524912 cites W2001718113 @default.
• W2000524912 cites W2004053712 @default.
• W2000524912 cites W2009798288 @default.
• W2000524912 cites W2017290170 @default.
• W2000524912 cites W2019344513 @default.
• W2000524912 cites W2027332646 @default.
• W2000524912 cites W2027484984 @default.
• W2000524912 cites W2031866624 @default.
• W2000524912 cites W2038157894 @default.
• W2000524912 cites W2039239878 @default.
• W2000524912 cites W2041089815 @default.
• W2000524912 cites W2048027697 @default.
• W2000524912 cites W2048942027 @default.
• W2000524912 cites W2057540177 @default.
• W2000524912 cites W2058226623 @default.
• W2000524912 cites W2060182219 @default.
• W2000524912 cites W2077124327 @default.
• W2000524912 cites W2086953830 @default.
• W2000524912 cites W2089668353 @default.
• W2000524912 cites W2093207947 @default.
• W2000524912 cites W2096763667 @default.
• W2000524912 cites W2097725719 @default.
• W2000524912 cites W2101703031 @default.
• W2000524912 cites W2103222461 @default.
• W2000524912 cites W2111496861 @default.
• W2000524912 cites W2114224063 @default.
• W2000524912 cites W2115385132 @default.
• W2000524912 cites W2115846884 @default.
• W2000524912 cites W2119978331 @default.
• W2000524912 cites W2121748291 @default.
• W2000524912 cites W2123937142 @default.
• W2000524912 cites W2136216384 @default.
• W2000524912 cites W2143996952 @default.
• W2000524912 cites W2148425951 @default.
• W2000524912 cites W2154964719 @default.
• W2000524912 cites W2155987222 @default.
• W2000524912 cites W2159568235 @default.
• W2000524912 cites W2161526892 @default.
• W2000524912 cites W2167491944 @default.
• W2000524912 cites W2170002207 @default.
• W2000524912 cites W2316646952 @default.
• W2000524912 cites W2327990018 @default.
• W2000524912 cites W4256425245 @default.
• W2000524912 doi "https://doi.org/10.1371/journal.pone.0117508" @default.
• W2000524912 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4320075" @default.
• W2000524912 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25658942" @default.
• W2000524912 hasPublicationYear "2015" @default.
• W2000524912 type Work @default.
• W2000524912 sameAs 2000524912 @default.
• W2000524912 citedByCount "17" @default.
• W2000524912 countsByYear W20005249122016 @default.
• W2000524912 countsByYear W20005249122018 @default.
• W2000524912 countsByYear W20005249122019 @default.
• W2000524912 countsByYear W20005249122020 @default.
• W2000524912 countsByYear W20005249122021 @default.
• W2000524912 countsByYear W20005249122022 @default.
• W2000524912 countsByYear W20005249122023 @default.
• W2000524912 crossrefType "journal-article" @default.
• W2000524912 hasAuthorship W2000524912A5011897079 @default.
• W2000524912 hasAuthorship W2000524912A5044938537 @default.
• W2000524912 hasAuthorship W2000524912A5061127730 @default.
• W2000524912 hasAuthorship W2000524912A5082389527 @default.
• W2000524912 hasAuthorship W2000524912A5086095536 @default.
• W2000524912 hasBestOaLocation W20005249121 @default.
• W2000524912 hasConcept C142724271 @default.
• W2000524912 hasConcept C147483822 @default.
• W2000524912 hasConcept C153911025 @default.
• W2000524912 hasConcept C159654299 @default.
• W2000524912 hasConcept C185592680 @default.
• W2000524912 hasConcept C188947578 @default.
• W2000524912 hasConcept C195616568 @default.
• W2000524912 hasConcept C203014093 @default.
• W2000524912 hasConcept C204787440 @default.
• W2000524912 hasConcept C2776895894 @default.
• W2000524912 hasConcept C2777327803 @default.
• W2000524912 hasConcept C2777349128 @default.

• next