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- W2000530007 abstract "Significance When starved for nutrients, diatoms redirect carbon toward biosynthesis of storage lipids, triacylglycerols (TAGs). We examined how this modification is achieved in the diatom Phaeodactylum tricornutum. Under nitrogen stress, the cells cannibalized their photosynthetic apparatus while recycling intracellular nitrogen and redirecting it to synthesize nitrogen assimilation enzymes. Simultaneously, they allocated newly fixed carbon toward lipids. In contrast, a nitrate reductase knocked-down strain shunted ∼40% more carbon toward TAGs than the wild type without losing photosynthetic capacity. Our results show that diatoms can remodel their intermediate metabolism on environmental cues and reveal that a key signal in this remodeling is associated with nitrogen assimilation. This insight informs a strategy of developing a much more efficient pathway to produce algal-based biofuels." @default.
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- W2000530007 date "2014-12-29" @default.
- W2000530007 modified "2023-10-14" @default.
- W2000530007 title "Remodeling of intermediate metabolism in the diatom <i>Phaeodactylum tricornutum</i> under nitrogen stress" @default.
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- W2000530007 doi "https://doi.org/10.1073/pnas.1419818112" @default.
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