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- W2000531214 abstract "This chapter discusses the aspects of amino acid transport in the central nervous system (CNS). It describes the transport of amino acids from the ventricular cavities to the nervous tissue. The preliminary results point to the presence of a barrier, the fluid-tissue barrier for amino acids in the central nervous system. The technique of ventriculo-cisternal perfusion is applied to study the disappearance of amino acids from the cerebrospinal fluid (CSF) avoiding the unspecific bulk flow through the arachnoid villi. In the experiment described in the chapter, the ventricular system was perfused in anesthetized cats with Merlis artificial CSF at a constant speed using a slow infusion pump at a rate of 66 μl/min—that is, 4 ml/h. For amino acids, the rate of infusion was 0.25 μM/min, which makes 15 μM/h. The effluent fluid was collected at 15-min intervals during a period of 2 hours controlling the inflow and outflow volumes. In the results, the existence of a barrier for amino acids between the CSF and the nervous parenchyma, the fluid-tissue barrier at the level of the ventricular cavities, were confirmed. Such a barrier could explain why the free amino acids of the brain do not pass into the CSF, maintaining a differential concentration gradient between the tissue and the fluid." @default.
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- W2000531214 date "1974-04-01" @default.
- W2000531214 modified "2023-09-27" @default.
- W2000531214 title "Variations in the manufacture of commercial silica gel plates" @default.
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- W2000531214 doi "https://doi.org/10.1016/s0021-9673(00)92548-2" @default.
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