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- W2000535917 abstract "Abstract A high-peformance liquid chromatography technique using UV detection is reported for the assay of anthranilate 2,3-dioxygenase (deaminating) activity. The substrate anthranilate, the main product 2,3-dihydroxybenzoate and the by-product 3-hydroxyanthranilate are determined quantitatively in the presence of biolgical material. After addition of benzoic acid as internal standard, the substances of interest are separated isocratically on a reversed-hase C 18 column with a detection sesitivity of 0.5 nmol and retention times of 3.5–10 min. A thin-layer chromatographic and fluorimetric method is also described." @default.
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- W2000535917 date "1982-08-01" @default.
- W2000535917 modified "2023-09-27" @default.
- W2000535917 title "Determination of anthranilate, 3-hydroxyanthranilate and 2,3-dihydroxybenzoate in the enzyme assay of anthranilate hydroxylase by thin-layer and high-performance liquid chromatography" @default.
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- W2000535917 doi "https://doi.org/10.1016/s0021-9673(00)82477-2" @default.
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