FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000536304> ?p ?o ?g. }

• W2000536304 endingPage "138" @default.
• W2000536304 startingPage "127" @default.
• W2000536304 abstract "The intratesticular concentration of progesterone (P) rises up to the micromolar range during high-dose luteinizing hormone (LH)/hCG stimulation. The aim of this study was to examine whether P is involved in the concomitant down-regulation of the LH receptor (R) function. The effects were tested in a mouse Leydig tumor cell line (mLTC-1) and in Percoll-purified adult mouse Leydig cells. Pre-incubation of the mLTC-1 cells for 48 h with P (1-10 micromol/l) decreased in dose-dependent fashion their specific binding of [125I]iodo-hCG as well as the hCG-induced cAMP production (down to 65 and 40% respectively, of controls, P < 0.01). Similar effect of P on hCG-induced cAMP production was observed in adult mouse Leydig cells following a 24 h incubation in the presence of P (0.3-10 micromol/l). In addition, P treatment significantly inhibited the expression of a transiently transfected murine LHR promoter (715 or 950 bp of the 5' untranslated region)-luciferase fusion constructs in mLTC-1 cells (down to 50% of control, P < 0.01). In accordance, a 6-12 h culture in the presence of 5-10 micromol/l of P showed significant down-regulatory effects on the steady state levels of LHR-mRNA in mLTC-1 cells. These inhibitory effects of P on the LHR expression and function were mimicked by similar concentrations of cortisol, but not by testosterone or estradiol. Blocking the steroid synthesis of mLTC-1 cells with 86 micromol/l of aminoglutethimide (AMG) partially reversed the down-regulating effect of hCG on the LHR-mRNA. Moreover, a 24 h culture in the presence of AMG showed an up-regulating effect on expression of the LHR promoter-luciferase constructs, and including hCG (50 microg/l) in the culture medium enhanced this effect. Hence, in the absence of steroidogenesis, hCG up-regulates the LHR promoter expression. In conclusion, we present here a novel short-loop regulatory mechanism in murine Leydig cells where P exerts a negative effect on LHR expression and function. Since Leydig cell P production is dramatically increased during high-dose stimulation with LH/hCG, due to blockade of C21 steroid side chain cleavage, the present findings offer a function for this steroid in the LHR down-regulation." @default.
• W2000536304 created "2016-06-24" @default.
• W2000536304 creator A5059776672 @default.
• W2000536304 creator A5088392007 @default.
• W2000536304 date "1998-02-01" @default.
• W2000536304 modified "2023-10-17" @default.
• W2000536304 title "Progesterone can participate in down-regulation of the luteinizing hormone receptor gene expression and function in cultured murine Leydig cells" @default.
• W2000536304 cites W1499455100 @default.
• W2000536304 cites W1526692496 @default.
• W2000536304 cites W1766046209 @default.
• W2000536304 cites W1935296246 @default.
• W2000536304 cites W1968790628 @default.
• W2000536304 cites W1981959892 @default.
• W2000536304 cites W1982684652 @default.
• W2000536304 cites W1982977098 @default.
• W2000536304 cites W1990974122 @default.
• W2000536304 cites W2001790486 @default.
• W2000536304 cites W2011130204 @default.
• W2000536304 cites W2017382801 @default.
• W2000536304 cites W2018889720 @default.
• W2000536304 cites W2026807241 @default.
• W2000536304 cites W2031148940 @default.
• W2000536304 cites W2034325295 @default.
• W2000536304 cites W2035749841 @default.
• W2000536304 cites W2038413192 @default.
• W2000536304 cites W2048441754 @default.
• W2000536304 cites W2050045288 @default.
• W2000536304 cites W2057936643 @default.
• W2000536304 cites W2058469225 @default.
• W2000536304 cites W2064135455 @default.
• W2000536304 cites W2064360396 @default.
• W2000536304 cites W2071170593 @default.
• W2000536304 cites W2082559087 @default.
• W2000536304 cites W2083694687 @default.
• W2000536304 cites W2084231330 @default.
• W2000536304 cites W2085109717 @default.
• W2000536304 cites W2091844597 @default.
• W2000536304 cites W2093204677 @default.
• W2000536304 cites W2095397678 @default.
• W2000536304 cites W2102225311 @default.
• W2000536304 cites W2108108269 @default.
• W2000536304 cites W2121317812 @default.
• W2000536304 cites W2140475850 @default.
• W2000536304 cites W2141219399 @default.
• W2000536304 cites W2157681486 @default.
• W2000536304 cites W2164074867 @default.
• W2000536304 cites W2166180405 @default.
• W2000536304 cites W4232253446 @default.
• W2000536304 cites W4232793818 @default.
• W2000536304 cites W4240453155 @default.
• W2000536304 cites W4240609540 @default.
• W2000536304 cites W4241547304 @default.
• W2000536304 cites W4241979030 @default.
• W2000536304 cites W4250789773 @default.
• W2000536304 cites W4252313334 @default.
• W2000536304 cites W4253401835 @default.
• W2000536304 doi "https://doi.org/10.1016/s0303-7207(98)00002-1" @default.
• W2000536304 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9605514" @default.
• W2000536304 hasPublicationYear "1998" @default.
• W2000536304 type Work @default.
• W2000536304 sameAs 2000536304 @default.
• W2000536304 citedByCount "49" @default.
• W2000536304 countsByYear W20005363042012 @default.
• W2000536304 countsByYear W20005363042014 @default.
• W2000536304 countsByYear W20005363042015 @default.
• W2000536304 countsByYear W20005363042018 @default.
• W2000536304 countsByYear W20005363042019 @default.
• W2000536304 countsByYear W20005363042020 @default.
• W2000536304 countsByYear W20005363042021 @default.
• W2000536304 countsByYear W20005363042022 @default.
• W2000536304 countsByYear W20005363042023 @default.
• W2000536304 crossrefType "journal-article" @default.
• W2000536304 hasAuthorship W2000536304A5059776672 @default.
• W2000536304 hasAuthorship W2000536304A5088392007 @default.
• W2000536304 hasConcept C121608353 @default.
• W2000536304 hasConcept C126322002 @default.
• W2000536304 hasConcept C134018914 @default.
• W2000536304 hasConcept C170493617 @default.
• W2000536304 hasConcept C24998067 @default.
• W2000536304 hasConcept C2775976807 @default.
• W2000536304 hasConcept C2776166826 @default.
• W2000536304 hasConcept C2777736315 @default.
• W2000536304 hasConcept C2778575703 @default.
• W2000536304 hasConcept C2779279991 @default.
• W2000536304 hasConcept C530470458 @default.
• W2000536304 hasConcept C54355233 @default.
• W2000536304 hasConcept C71315377 @default.
• W2000536304 hasConcept C71924100 @default.
• W2000536304 hasConcept C81885089 @default.
• W2000536304 hasConcept C86803240 @default.
• W2000536304 hasConceptScore W2000536304C121608353 @default.
• W2000536304 hasConceptScore W2000536304C126322002 @default.
• W2000536304 hasConceptScore W2000536304C134018914 @default.
• W2000536304 hasConceptScore W2000536304C170493617 @default.
• W2000536304 hasConceptScore W2000536304C24998067 @default.
• W2000536304 hasConceptScore W2000536304C2775976807 @default.
• W2000536304 hasConceptScore W2000536304C2776166826 @default.
• W2000536304 hasConceptScore W2000536304C2777736315 @default.

• next