FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000536484> ?p ?o ?g. }

• W2000536484 endingPage "102" @default.
• W2000536484 startingPage "96" @default.
• W2000536484 abstract "The detection, analysis, and understanding of protein complexes/aggregates and their formation process are extremely important for biomolecular research, diagnosis, and biopharmaceutical development. Unfortunately, techniques that can be used conveniently for protein complex/aggregate detection and analysis are very limited. Using gold nanoparticle immunoprobes coupled with dynamic light scattering (DLS), we developed a label-free nanoparticle aggregation immunoassay (NanoDLSay) for protein aggregate detection and study. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH), a protein target used routinely in Western blot as a loading control, is demonstrated here as an example. Through this study, we discovered that GAPDH has a strong tendency to form large aggregates in certain buffer solutions at a concentration range of 10–25 μg/ml. The strong light scattering property of gold nanoparticles immunoprobes greatly enhanced the sensitivity of the dynamic light scattering for protein complex/aggregate detection. In contrast to fluorescence techniques for protein complex and aggregation study, the protein targets do not need to be labeled with fluorescent probe molecules in NanoDLSay. NanoDLSay is a very convenient and sensitive tool for protein complex/aggregate detection and study." @default.
• W2000536484 created "2016-06-24" @default.
• W2000536484 creator A5028709782 @default.
• W2000536484 creator A5070135836 @default.
• W2000536484 creator A5087726580 @default.
• W2000536484 creator A5090463910 @default.
• W2000536484 date "2010-10-01" @default.
• W2000536484 modified "2023-10-16" @default.
• W2000536484 title "A label-free nanoparticle aggregation assay for protein complex/aggregate detection and study" @default.
• W2000536484 cites W1026240615 @default.
• W2000536484 cites W1497273660 @default.
• W2000536484 cites W1758747526 @default.
• W2000536484 cites W1971227936 @default.
• W2000536484 cites W1977186059 @default.
• W2000536484 cites W2003596928 @default.
• W2000536484 cites W2009562525 @default.
• W2000536484 cites W2030626080 @default.
• W2000536484 cites W2031095951 @default.
• W2000536484 cites W2038665562 @default.
• W2000536484 cites W2042823734 @default.
• W2000536484 cites W2047583225 @default.
• W2000536484 cites W2068897525 @default.
• W2000536484 cites W2069610017 @default.
• W2000536484 cites W2092859042 @default.
• W2000536484 cites W2123631149 @default.
• W2000536484 cites W2124112137 @default.
• W2000536484 cites W2132195689 @default.
• W2000536484 cites W2140288745 @default.
• W2000536484 cites W2157561719 @default.
• W2000536484 cites W3038632501 @default.
• W2000536484 cites W4254630790 @default.
• W2000536484 cites W4313375559 @default.
• W2000536484 doi "https://doi.org/10.1016/j.ab.2010.06.008" @default.
• W2000536484 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20553869" @default.
• W2000536484 hasPublicationYear "2010" @default.
• W2000536484 type Work @default.
• W2000536484 sameAs 2000536484 @default.
• W2000536484 citedByCount "30" @default.
• W2000536484 countsByYear W20005364842012 @default.
• W2000536484 countsByYear W20005364842013 @default.
• W2000536484 countsByYear W20005364842014 @default.
• W2000536484 countsByYear W20005364842015 @default.
• W2000536484 countsByYear W20005364842016 @default.
• W2000536484 countsByYear W20005364842017 @default.
• W2000536484 countsByYear W20005364842018 @default.
• W2000536484 countsByYear W20005364842019 @default.
• W2000536484 countsByYear W20005364842020 @default.
• W2000536484 crossrefType "journal-article" @default.
• W2000536484 hasAuthorship W2000536484A5028709782 @default.
• W2000536484 hasAuthorship W2000536484A5070135836 @default.
• W2000536484 hasAuthorship W2000536484A5087726580 @default.
• W2000536484 hasAuthorship W2000536484A5090463910 @default.
• W2000536484 hasConcept C104819515 @default.
• W2000536484 hasConcept C121332964 @default.
• W2000536484 hasConcept C12554922 @default.
• W2000536484 hasConcept C134792803 @default.
• W2000536484 hasConcept C136238340 @default.
• W2000536484 hasConcept C14631669 @default.
• W2000536484 hasConcept C155672457 @default.
• W2000536484 hasConcept C159654299 @default.
• W2000536484 hasConcept C171250308 @default.
• W2000536484 hasConcept C181199279 @default.
• W2000536484 hasConcept C185592680 @default.
• W2000536484 hasConcept C192562407 @default.
• W2000536484 hasConcept C203014093 @default.
• W2000536484 hasConcept C2776317432 @default.
• W2000536484 hasConcept C2780420688 @default.
• W2000536484 hasConcept C2781047461 @default.
• W2000536484 hasConcept C43617362 @default.
• W2000536484 hasConcept C54355233 @default.
• W2000536484 hasConcept C55493867 @default.
• W2000536484 hasConcept C62520636 @default.
• W2000536484 hasConcept C86803240 @default.
• W2000536484 hasConcept C91881484 @default.
• W2000536484 hasConceptScore W2000536484C104819515 @default.
• W2000536484 hasConceptScore W2000536484C121332964 @default.
• W2000536484 hasConceptScore W2000536484C12554922 @default.
• W2000536484 hasConceptScore W2000536484C134792803 @default.
• W2000536484 hasConceptScore W2000536484C136238340 @default.
• W2000536484 hasConceptScore W2000536484C14631669 @default.
• W2000536484 hasConceptScore W2000536484C155672457 @default.
• W2000536484 hasConceptScore W2000536484C159654299 @default.
• W2000536484 hasConceptScore W2000536484C171250308 @default.
• W2000536484 hasConceptScore W2000536484C181199279 @default.
• W2000536484 hasConceptScore W2000536484C185592680 @default.
• W2000536484 hasConceptScore W2000536484C192562407 @default.
• W2000536484 hasConceptScore W2000536484C203014093 @default.
• W2000536484 hasConceptScore W2000536484C2776317432 @default.
• W2000536484 hasConceptScore W2000536484C2780420688 @default.
• W2000536484 hasConceptScore W2000536484C2781047461 @default.
• W2000536484 hasConceptScore W2000536484C43617362 @default.
• W2000536484 hasConceptScore W2000536484C54355233 @default.
• W2000536484 hasConceptScore W2000536484C55493867 @default.
• W2000536484 hasConceptScore W2000536484C62520636 @default.
• W2000536484 hasConceptScore W2000536484C86803240 @default.
• W2000536484 hasConceptScore W2000536484C91881484 @default.
• W2000536484 hasIssue "1" @default.
• W2000536484 hasLocation W20005364841 @default.

• next