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- W2000544071 abstract "Somatic embryogenesis occurs spontaneously in some monocotyledoneous callus and cell suspension cultures maintained in suitable culture conditions. Nevertherless, the processes involved in somatic embryo development, and factors inducing this differentiation, are poorly understood. In order to study the changes in protein composition accompanying embryogenesis in cell suspension cultures of Dactylis glomerata L., embryos of various sizes and “undifferentiated” callus cells were separated and their total cellular protein extracts analyzed by two-dimensional polyacrylamide gel electrophoresis. Several proteins could be identified that are specific for embryos or callus under various culture conditions. Three independent detection methods were employed: silver-staining of proteins, in vivo labeling of proteins with [35S]methionine, and in vitro translation of poly(A)+ RNA. All culture conditions tested, including those that induce embryonic proteins in carrot, fail to induce embryonic proteins in D. glomerata callus cells." @default.
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- W2000544071 date "1988-01-01" @default.
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- W2000544071 title "Embryogenic and callus-specific proteins in somatic embryogenesis of the grass, Dactylis glomerata L." @default.
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- W2000544071 doi "https://doi.org/10.1016/0168-9452(88)90070-2" @default.
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