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- W2000544249 abstract "Abstract The modes of degradation of [14C]starch at different temperatures in Chlorella vulgaris cells were studied by means of gel chromatography and radio-gas liquid chromatography. When [14C]starch produced by photosynthesis at 20°C in the legal cells was degraded in the dark, the distribution on molecular size showed two maxima, M w > 2 × 10 6 (L-starch) and M w ⩾ 10 4 (S-starch), at all temperatures from 10° to 38°C. Although practically no radioactivity was lost from the total starch fraction at 10°C at 20°C, L-starch was converted to S-starch without any remarkable accumulation of the intermediate molecular size of starch. The initial rate of the conversion of L-starch to S-starch increased with increasing temperatures until 38°C. With degradation of L-starch, α-1,6-glycosidic linkage decreased and the non-reducing end of α-glucan increased. The results suggest that a debranching enzyme as well as α-amylase play principal role in the temperature-dependent degradation of starch in Chlorella. At temperatures as high as 38°C, 14C was greatly lost from the total starch fraction. This suggests that at high temperatures starch was markedly degraded by α-glucan phosphorylase together with the transfomation from L-starch to S-starch." @default.
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- W2000544249 date "1983-07-01" @default.
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- W2000544249 title "Change in molecular weight distribution in starch when degraded at different temperatures in Chlorella vulgaris" @default.
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- W2000544249 doi "https://doi.org/10.1016/0304-4211(83)90164-5" @default.
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