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- W2000545888 abstract "Abstract Vitamind D was separated from percalciferol within 1.2 min with a high-efficiency adsorptive liquid chromatographic suppor (Vydac) and a low viscosity solvent. The efficiency of the chromatographic system was studied as a function of solvent viscosity and flow-rate. In agreement with Van Deemter's and Giddings' equations for the theoretical plate height, decreasing flow-rate and solvent viscosity enhanced the efficiency of the column. However, in the presence of low viscosity solvents, the observed relationship between plate height and flow-rate could be interpreted best by Giddings' equation for eddy current and mass transfer “coupling”. The chromatographic system was applied to (1) separation of vitamin A acetate, palmitate, aldehyde, alcohol and vitamin E from vitamin D and (2) a study of cholecalciferol isomerization. The observed isomerization equilibrium constants are in a greement with previously published results." @default.
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- W2000545888 date "1972-12-01" @default.
- W2000545888 modified "2023-09-25" @default.
- W2000545888 title "High-efficiency liquid chromatographic separation of vitamin D from precalciferol" @default.
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- W2000545888 doi "https://doi.org/10.1016/s0021-9673(01)94971-4" @default.
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