Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000546211> ?p ?o ?g. }
Showing items 1 to 88 of
88
with 100 items per page.
- W2000546211 endingPage "578" @default.
- W2000546211 startingPage "570" @default.
- W2000546211 abstract "Site-directed mutagenesis in which individual cleavage site P1 amino acids were changed to Ala was performed to delineate their importance in the processing of pro-CCK in mouse pituitary tumor AtT-20 cells. Individual substitution of cleavage sites on pro-CCK, viz., CCK 58 cleavage site R/A to A/A, CCK 33 cleavage site R/K to A/K, CCK 22 cleavage site K/N to A/N, and CCK 8 cleavage site R/D to A/D, did not inhibit pro-CCK expression or the production of some form of amidated CCK. Wild-type CCK cDNA expression in these cells results in production and secretion of CCK 8 and CCK 22. Substitution of the 58R/A cleavage site with A/A produces only CCK 33; 33A/K and 22A/N produce only CCK 8, whereas 8A/D produces CCK 12 and some CCK 22. Where the GRR residues on the C-terminus of CCK 8 were mutated to GAA, no amidated CCK was produced. Significant amounts of the pro-CCK, C-terminal peptide S9S was found in the medium of cells transfected with GAA mutant cDNA, indicating that this pro-CCK was cleaved at the GAA site probably by a nonprohormone convertase enzyme. Further analysis of the cells expressing the GAA mutant demonstrated that it is not extensively cleaved at other sites to produce CCK 8 GAA or larger peptides. In the mutant where the entire pro-CCK, C-terminal S9S was deleted, CCK 8 is processed and secreted normally. Thus, the cleavage at the C-terminal GRR site is essential for subsequent cleavages, and modification of other cleavage sites (58, 33, 22, and 8) has a major impact on pro-CCK processing. These results suggest that there is a temporal order of cleavages, and the structure of pro-CCK has a strong influence on where and whether pro-CCK is processed." @default.
- W2000546211 created "2016-06-24" @default.
- W2000546211 creator A5051336724 @default.
- W2000546211 creator A5077735430 @default.
- W2000546211 date "2001-12-14" @default.
- W2000546211 modified "2023-09-24" @default.
- W2000546211 title "Biosynthesis and Post-Translational Processing of Site-Directed Endoproteolytic Cleavage Mutants of Pro-CCK in AtT-20 Cells" @default.
- W2000546211 cites W1482927441 @default.
- W2000546211 cites W1518908263 @default.
- W2000546211 cites W1534631025 @default.
- W2000546211 cites W1534644359 @default.
- W2000546211 cites W1570075490 @default.
- W2000546211 cites W1883375123 @default.
- W2000546211 cites W2022527113 @default.
- W2000546211 cites W2034643350 @default.
- W2000546211 cites W2061372978 @default.
- W2000546211 cites W2062808764 @default.
- W2000546211 cites W2070287146 @default.
- W2000546211 cites W2077449996 @default.
- W2000546211 cites W2087328208 @default.
- W2000546211 cites W2334061140 @default.
- W2000546211 cites W4244906151 @default.
- W2000546211 doi "https://doi.org/10.1021/bi015566u" @default.
- W2000546211 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11781096" @default.
- W2000546211 hasPublicationYear "2001" @default.
- W2000546211 type Work @default.
- W2000546211 sameAs 2000546211 @default.
- W2000546211 citedByCount "9" @default.
- W2000546211 countsByYear W20005462112013 @default.
- W2000546211 crossrefType "journal-article" @default.
- W2000546211 hasAuthorship W2000546211A5051336724 @default.
- W2000546211 hasAuthorship W2000546211A5077735430 @default.
- W2000546211 hasConcept C103408237 @default.
- W2000546211 hasConcept C104317684 @default.
- W2000546211 hasConcept C143065580 @default.
- W2000546211 hasConcept C151730666 @default.
- W2000546211 hasConcept C153911025 @default.
- W2000546211 hasConcept C16318435 @default.
- W2000546211 hasConcept C170493617 @default.
- W2000546211 hasConcept C175156509 @default.
- W2000546211 hasConcept C181199279 @default.
- W2000546211 hasConcept C185592680 @default.
- W2000546211 hasConcept C187882448 @default.
- W2000546211 hasConcept C2777265534 @default.
- W2000546211 hasConcept C2777593968 @default.
- W2000546211 hasConcept C43369102 @default.
- W2000546211 hasConcept C54009773 @default.
- W2000546211 hasConcept C55493867 @default.
- W2000546211 hasConcept C86803240 @default.
- W2000546211 hasConceptScore W2000546211C103408237 @default.
- W2000546211 hasConceptScore W2000546211C104317684 @default.
- W2000546211 hasConceptScore W2000546211C143065580 @default.
- W2000546211 hasConceptScore W2000546211C151730666 @default.
- W2000546211 hasConceptScore W2000546211C153911025 @default.
- W2000546211 hasConceptScore W2000546211C16318435 @default.
- W2000546211 hasConceptScore W2000546211C170493617 @default.
- W2000546211 hasConceptScore W2000546211C175156509 @default.
- W2000546211 hasConceptScore W2000546211C181199279 @default.
- W2000546211 hasConceptScore W2000546211C185592680 @default.
- W2000546211 hasConceptScore W2000546211C187882448 @default.
- W2000546211 hasConceptScore W2000546211C2777265534 @default.
- W2000546211 hasConceptScore W2000546211C2777593968 @default.
- W2000546211 hasConceptScore W2000546211C43369102 @default.
- W2000546211 hasConceptScore W2000546211C54009773 @default.
- W2000546211 hasConceptScore W2000546211C55493867 @default.
- W2000546211 hasConceptScore W2000546211C86803240 @default.
- W2000546211 hasIssue "2" @default.
- W2000546211 hasLocation W20005462111 @default.
- W2000546211 hasLocation W20005462112 @default.
- W2000546211 hasOpenAccess W2000546211 @default.
- W2000546211 hasPrimaryLocation W20005462111 @default.
- W2000546211 hasRelatedWork W1964489770 @default.
- W2000546211 hasRelatedWork W1975766203 @default.
- W2000546211 hasRelatedWork W2011097917 @default.
- W2000546211 hasRelatedWork W2013630470 @default.
- W2000546211 hasRelatedWork W2053583806 @default.
- W2000546211 hasRelatedWork W2078490046 @default.
- W2000546211 hasRelatedWork W2124597856 @default.
- W2000546211 hasRelatedWork W2279156773 @default.
- W2000546211 hasRelatedWork W258580054 @default.
- W2000546211 hasRelatedWork W4233434341 @default.
- W2000546211 hasVolume "41" @default.
- W2000546211 isParatext "false" @default.
- W2000546211 isRetracted "false" @default.
- W2000546211 magId "2000546211" @default.
- W2000546211 workType "article" @default.